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Literature summary for 1.1.99.13 extracted from
- Purification und properties of 3-ketosucrose-forming enzyme from the cells of Agrobacterium tumefaciens (1967), J. Biol. Chem., 242, 3665-3672.
No PubMed abstract available
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| Atabrine | activation at acidic pH-values | Agrobacterium tumefaciens |  |
General Stability
| General Stability | Organism |
|---|---|
| sucrose protects against inactivation in acidic media | Agrobacterium tumefaciens |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| Ca2+ | complete | Agrobacterium tumefaciens | |
| Cu2+ | - |
Agrobacterium tumefaciens | |
| L-arabinose | slight | Agrobacterium tumefaciens | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.016 | - |
D-glucose | - |
Agrobacterium tumefaciens | |
| 0.038 | - |
sucrose | - |
Agrobacterium tumefaciens | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| cytoplasm | - |
Agrobacterium tumefaciens | 5737 | - |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| aldohexosides + acceptor | Agrobacterium tumefaciens | reaction in sugar metabolism | ? | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Agrobacterium tumefaciens | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| partially | Agrobacterium tumefaciens |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 2-deoxy-D-glucose + acceptor | - |
Agrobacterium tumefaciens | 2-deoxy-3-dehydro-D-glucose + reduced acceptor | - |
? | |
| aldohexosides + acceptor | reaction in sugar metabolism | Agrobacterium tumefaciens | ? | - |
? | |
| alpha-methyl-D-glucoside + acceptor | - |
Agrobacterium tumefaciens | 3-keto-alpha-methyl-glucoside + reduced acceptor | - |
? | |
| beta-melibiose + acceptor | - |
Agrobacterium tumefaciens | ? | - |
? | |
| cellobiose + acceptor | - |
Agrobacterium tumefaciens | ? | - |
? | |
| D-galactose + acceptor | - |
Agrobacterium tumefaciens | 3-dehydro-D-galactose + reduced acceptor | - |
? | |
| D-galactosides + acceptor | - |
Agrobacterium tumefaciens | 3-ketogalactosides + reduced acceptor | - |
? | |
| D-glucosamine + acceptor | - |
Agrobacterium tumefaciens | 3-dehydro-D-glucosamine + reduced acceptor | - |
? | |
| D-glucose + acceptor | - |
Agrobacterium tumefaciens | 3-ketoglucose + reduced acceptor | D-ribohexos-3-ulose | ? | |
| D-glucose 1-phosphate + acceptor | not D-glucose 6-phosphate | Agrobacterium tumefaciens | 3-keto-D-glucose 1-phosphate + reduced acceptor | - |
? | |
| D-glucosides + acceptor | react more rapidly than galactosides, aldopentoses and methylpentoses are no substrates | Agrobacterium tumefaciens | 3-ketoglucosides + reduced acceptor | - |
? | |
| D-sorbitol + acceptor | - |
Agrobacterium tumefaciens | ? | - |
? | |
| lactose + acceptor | - |
Agrobacterium tumefaciens | ? | - |
? | |
| maltose + acceptor | - |
Agrobacterium tumefaciens | ? | - |
? | |
| raffinose + acceptor | - |
Agrobacterium tumefaciens | ? | - |
? | |
| salicin + acceptor | - |
Agrobacterium tumefaciens | ? | - |
? | |
| sucrose + acceptor | - |
Agrobacterium tumefaciens | 3-dehydro-alpha-D-glucosyl-beta-D-fructofuranoside + reduced acceptor | 3-ketosucrose, alpha-D-ribo-hexopyranosyl-3-ulose-beta-fructofuranoside | ? | |
| trehalose + acceptor | - |
Agrobacterium tumefaciens | ? | - |
? | |
| UDP-glucose + acceptor | - |
Agrobacterium tumefaciens | UDP-3-dehydro-D-glucose + reduced acceptor | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| D-glucoside 3-dehydrogenase | - |
Agrobacterium tumefaciens |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 20 | - |
enzyme assay | Agrobacterium tumefaciens |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 6 | 6.7 | - |
Agrobacterium tumefaciens |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 5.2 | 7.2 | 85% of maximal activity at pH 5.2 and 7.2 | Agrobacterium tumefaciens |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 6 | - |
and below, labile, sucrose stabilizes | Agrobacterium tumefaciens |
| 7 | 9 | stable | Agrobacterium tumefaciens |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| FAD | flavoprotein | Agrobacterium tumefaciens | |
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