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Literature summary for 1.1.99.12 extracted from

  • Asakura, A.; Hoshino, T.
    Isolation and Characterization of a New Quinoprotein Dehydrogenase, L-Sorbose/L-Sorbosone Dehydrogenase. (1999), Biosci. Biotechnol. Biochem., 63, 46-53.
    View publication on PubMed
Search for more literature for 1.1.99.12

Inhibitors

Inhibitors Comment Organism Structure
CoCl2 strain DSM 4025, 5 mM, 84% inhibition Gluconobacter oxydans
EDTA strain DSM 4025, 5 mM, 87% inhibition Gluconobacter oxydans
EGTA strain DSM 4025, 5 mM, 89% inhibition Gluconobacter oxydans
FeCl2 strain DSM 4025, 5 mM, 89% inhibition Gluconobacter oxydans
FeCl3 strain DSM 4025, 5 mM, 97% inhibition Gluconobacter oxydans
NiSO4 strain DSM 4025, 5 mM, 77% inhibition Gluconobacter oxydans
ZnCl2 strain DSM 4025, 5 mM, 90% inhibition Gluconobacter oxydans

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2
-
 1-propanol strain DSM 4025 Gluconobacter oxydans
230
-
 L-sorbose strain DSM 4025 Gluconobacter oxydans

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
62500
-
 strain DSM 4025, 1 * 62500 + 1 * 64500, SDS-PAGE Gluconobacter oxydans
64500
-
 strain DSM 4025, 1 * 62500 + 1 * 64500, SDS-PAGE Gluconobacter oxydans
135000
-
 strain DSM 4025, gel filtration Gluconobacter oxydans

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
L-sorbose + acceptor Gluconobacter oxydans strain DSM 4025, enzyme has both L-sorbose dehydrogenase and L-sorbosone dehydrogenase activity, 10.9% of activity with 1-propanol L-sorbosone + reduced acceptor
-
 ?

Organism

Organism UniProt Comment Textmining
Gluconobacter oxydans
-
 strain DSM 4025
-

Purification (Commentary)

Purification (Comment) Organism
strain DSM 4025, PEG 600, DEAE-Toyopearl, Q-Sepharose Gluconobacter oxydans

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
6.73
-
 strain DSM 4025 Gluconobacter oxydans

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1-butanol + acceptor strain DSM 4025, 69.4% of activity with 1-propanol Gluconobacter oxydans n-butanal + reduced acceptor
-
 ?
1-pentanol + acceptor strain DSM 4025, 49.8% of activity with 1-propanol Gluconobacter oxydans n-pentanal + reduced acceptor
-
 ?
1-propanol + acceptor
-
 Gluconobacter oxydans n-propanal + reduced acceptor
-
 ?
2-butanol + acceptor strain DSM 4025, 87.4% of activity with 1-propanol Gluconobacter oxydans 2-butanone + reduced acceptor
-
 ?
2-propanol + acceptor strain DSM 4025, 78.2% of activity with 1-propanol Gluconobacter oxydans acetone + reduced acceptor
-
 ?
D-fructose + acceptor strain DSM 4025, 6.6% of activity with 1-propanol Gluconobacter oxydans 2-keto-D-gulonate + reduced acceptor
-
 ?
D-glucose + acceptor strain DSM 4025, 25.3% of activity with 1-propanol Gluconobacter oxydans D-gluconic acid + reduced acceptor
-
 ?
D-mannitol + acceptor strain DSM 4025, 16.2% of activity with 1-propanol Gluconobacter oxydans ? + reduced acceptor
-
 ?
D-sorbitol + acceptor strain DSM 4025, 7.1% of activity with 1-propanol Gluconobacter oxydans D-glucose + D-gluconic acid + L-sorbose + reduced acceptor
-
 ?
DL-glyceraldehyde + acceptor strain DSM 4025, 80.2% of activity with 1-propanol Gluconobacter oxydans ? + reduced acceptor
-
 ?
ethanol + acceptor strain DSM 4025, 88.6% of activity with 1-propanol Gluconobacter oxydans ethanal + reduced acceptor
-
 ?
L-sorbose + acceptor strain DSM 4025, enzyme has both L-sorbose dehydrogenase and L-sorbosone dehydrogenase activity, 10.9% of activity with 1-propanol Gluconobacter oxydans L-sorbosone + reduced acceptor
-
 ?
L-sorbosone + acceptor strain DSM 4025, 16.2% of activity with 1-propanol Gluconobacter oxydans 2-keto-L-gulonate + reduced acceptor
-
 ?
n-heptanol + acceptor strain DSM 4025, 47.7% of activity with 1-propanol Gluconobacter oxydans n-heptanal + reduced acceptor
-
 ?
n-hexanol + acceptor strain DSM 4025, 49.1% of activity with 1-propanol Gluconobacter oxydans n-hexanal + reduced acceptor
-
 ?
polyethylene glycol 100 + acceptor strain DSM 4025, 68.9% of activity with 1-propanol Gluconobacter oxydans ?
-
 ?
polyvinyl alcohol + acceptor strain DSM 4025, 38.1% of activity with 1-propanol Gluconobacter oxydans oxidized polyvinyl alcohol + reduced acceptor
-
 ?
propionaldehyde + acceptor strain DSM 4025, 83.3% of activity with 1-propanol Gluconobacter oxydans propanoate + reduced acceptor
-
 ?

Subunits

Subunits Comment Organism
dimer strain DSM 4025, 1 * 62500 + 1 * 64500, SDS-PAGE Gluconobacter oxydans

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30 40 strain DSM 4025 Gluconobacter oxydans

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
10 50 strain DSM 4025 Gluconobacter oxydans

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
additional information
-
 strain DSM 4025, enzyme is stable at and below 30°C, but unstable above 40°C Gluconobacter oxydans

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7 9 strain DSM 4025 Gluconobacter oxydans

pH Stability

pH Stability pH Stability Maximum Comment Organism
additional information
-
 strain DSM 4025, enzyme is stable in alkaline pH and unstable with increasing acidity Gluconobacter oxydans

Cofactor

Cofactor Comment Organism Structure
cytochrome c strain DSM 4025, physiological electron acceptor Gluconobacter oxydans
pyrroloquinoline quinone strain DSM 4025, non-covalently bound, 2 mol PQQ/mol enzyme Gluconobacter oxydans