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Literature summary for 1.1.99.1 extracted from

  • Russell, R.; Scopes, R.K.
    Use of hydrophobic chromatography for purification of the membrane-located choline dehydrogenase from a Pseudomonas strain (1994), Bioseparation, 4, 279-284.
    View publication on PubMed
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KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2
-
 choline in the presence of 0.05 mM phenazine methosulfate Pseudomonas aeruginosa

Localization

Localization Comment Organism GeneOntology No. Textmining
membrane
-
 Pseudomonas aeruginosa 16020
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
60000 70000 gel filtration Pseudomonas aeruginosa
66000
-
 1 * 66000, SDS-PAGE Pseudomonas aeruginosa

Organism

Organism UniProt Comment Textmining
Pseudomonas aeruginosa
-
 C10 strain
-
Pseudomonas aeruginosa C10
-
 C10 strain
-

Purification (Commentary)

Purification (Comment) Organism
using column chromatography on thio-hexyl and on an anion exchanger Pseudomonas aeruginosa

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
67
-
-
 Pseudomonas aeruginosa

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
choline + acceptor electron acceptor: phenazine methosulfate Pseudomonas aeruginosa betaine aldehyde + reduced acceptor
-
 ?
choline + acceptor electron acceptor: phenazine methosulfate Pseudomonas aeruginosa C10 betaine aldehyde + reduced acceptor
-
 ?

Subunits

Subunits Comment Organism
monomer 1 * 66000, SDS-PAGE Pseudomonas aeruginosa

Cofactor

Cofactor Comment Organism Structure
pyrroloquinoline quinone
-
 Pseudomonas aeruginosa