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Literature summary for 1.1.99.1 extracted from

  • Tsuge, H.; Nakano, Y.; Onishi, H.; Futamura, Y.; Ohashi, K.
    A novel purification and some properties of rat liver mitochondrial choline dehydrogenase (1980), Biochim. Biophys. Acta, 614, 274-284.
    View publication on PubMed

Activating Compound

Activating Compound Comment Organism Structure
coenzyme Q primary electron acceptor in vivo Rattus norvegicus

General Stability

General Stability Organism
repeated freezing and thawing: rapid loss of activity Rattus norvegicus

Inhibitors

Inhibitors Comment Organism Structure
2-Dimethylaminoethanol
-
Rattus norvegicus
Betaine aldehyde choline dehydrogenase activity Rattus norvegicus
DL-3-hydroxybutyrate at 16.7 mM acts as weak inhibitor Rattus norvegicus
Monoethanolamine
-
Rattus norvegicus
Semicarbazide
-
Rattus norvegicus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.1
-
phenazine methosulfate with choline Rattus norvegicus
3.1
-
Betaine aldehyde at 1 mM phenazine methosulfate Rattus norvegicus
7
-
choline electron acceptor: phenazine methosulfate Rattus norvegicus

Localization

Localization Comment Organism GeneOntology No. Textmining
mitochondrion
-
Rattus norvegicus 5739
-

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
choline + coenzyme Q1 Rattus norvegicus mitochondrial oxidation of choline betaine aldehyde + reduced coenzyme Q1
-
?

Organism

Organism UniProt Comment Textmining
Rattus norvegicus
-
-
-

Purification (Commentary)

Purification (Comment) Organism
using column chromatography of the chemically modified enzyme with 5,5'-dithiobis(2-nitrobenzoic acid) on DEAE-Sepharose CL-6B and choline-Sepharose 4B with C3-spacer, subsequent release of the thionitrobenzoate with dithiothreitol, and a second column chromatography on DEAE-Sepharose CL-6B in the presence of 0.1% Triton X-100 Rattus norvegicus

Source Tissue

Source Tissue Comment Organism Textmining
liver
-
Rattus norvegicus
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
6.3
-
-
Rattus norvegicus

Storage Stability

Storage Stability Organism
-15°C, 50% loss of activity, 1 month Rattus norvegicus
-20°C, stable, partially purified enzyme Rattus norvegicus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
choline + acceptor the enzyme can use only choline and betaine aldehyde Rattus norvegicus betaine aldehyde + reduced acceptor
-
r
choline + acceptor absolute requirement for an electron acceptor other than O2 Rattus norvegicus betaine aldehyde + reduced acceptor
-
r
choline + acceptor reverse reaction at 5.2% of forward reaction Rattus norvegicus betaine aldehyde + reduced acceptor
-
r
choline + acceptor coenzyme Q, primary electron acceptor in vivo Rattus norvegicus betaine aldehyde + reduced acceptor
-
r
choline + acceptor electron acceptor: phenazine methosulfate Rattus norvegicus betaine aldehyde + reduced acceptor
-
r
choline + acceptor not: 2-dimethylaminoethanol, monoethanolamine Rattus norvegicus betaine aldehyde + reduced acceptor
-
r
choline + coenzyme Q1 mitochondrial oxidation of choline Rattus norvegicus betaine aldehyde + reduced coenzyme Q1
-
?
choline + phenazine methosulfate
-
Rattus norvegicus betaine aldehyde + reduced phenazine methosulfate
-
r

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
45
-
-
Rattus norvegicus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
9
-
-
Rattus norvegicus

Cofactor

Cofactor Comment Organism Structure
coenzyme Q primary electron acceptor in vivo Rattus norvegicus

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
1
-
2-Dimethylaminoethanol inhibits the enzyme competitively Rattus norvegicus
3.3
-
Betaine aldehyde inhibits the oxidation of choline competitively Rattus norvegicus