Activating Compound | Comment | Organism | Structure |
---|---|---|---|
coenzyme Q | primary electron acceptor in vivo | Rattus norvegicus |
General Stability | Organism |
---|---|
repeated freezing and thawing: rapid loss of activity | Rattus norvegicus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
2-Dimethylaminoethanol | - |
Rattus norvegicus | |
Betaine aldehyde | choline dehydrogenase activity | Rattus norvegicus | |
DL-3-hydroxybutyrate | at 16.7 mM acts as weak inhibitor | Rattus norvegicus | |
Monoethanolamine | - |
Rattus norvegicus | |
Semicarbazide | - |
Rattus norvegicus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
1.1 | - |
phenazine methosulfate | with choline | Rattus norvegicus | |
3.1 | - |
Betaine aldehyde | at 1 mM phenazine methosulfate | Rattus norvegicus | |
7 | - |
choline | electron acceptor: phenazine methosulfate | Rattus norvegicus |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
mitochondrion | - |
Rattus norvegicus | 5739 | - |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
choline + coenzyme Q1 | Rattus norvegicus | mitochondrial oxidation of choline | betaine aldehyde + reduced coenzyme Q1 | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Rattus norvegicus | - |
- |
- |
Purification (Comment) | Organism |
---|---|
using column chromatography of the chemically modified enzyme with 5,5'-dithiobis(2-nitrobenzoic acid) on DEAE-Sepharose CL-6B and choline-Sepharose 4B with C3-spacer, subsequent release of the thionitrobenzoate with dithiothreitol, and a second column chromatography on DEAE-Sepharose CL-6B in the presence of 0.1% Triton X-100 | Rattus norvegicus |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
liver | - |
Rattus norvegicus | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
6.3 | - |
- |
Rattus norvegicus |
Storage Stability | Organism |
---|---|
-15°C, 50% loss of activity, 1 month | Rattus norvegicus |
-20°C, stable, partially purified enzyme | Rattus norvegicus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
choline + acceptor | the enzyme can use only choline and betaine aldehyde | Rattus norvegicus | betaine aldehyde + reduced acceptor | - |
r | |
choline + acceptor | absolute requirement for an electron acceptor other than O2 | Rattus norvegicus | betaine aldehyde + reduced acceptor | - |
r | |
choline + acceptor | reverse reaction at 5.2% of forward reaction | Rattus norvegicus | betaine aldehyde + reduced acceptor | - |
r | |
choline + acceptor | coenzyme Q, primary electron acceptor in vivo | Rattus norvegicus | betaine aldehyde + reduced acceptor | - |
r | |
choline + acceptor | electron acceptor: phenazine methosulfate | Rattus norvegicus | betaine aldehyde + reduced acceptor | - |
r | |
choline + acceptor | not: 2-dimethylaminoethanol, monoethanolamine | Rattus norvegicus | betaine aldehyde + reduced acceptor | - |
r | |
choline + coenzyme Q1 | mitochondrial oxidation of choline | Rattus norvegicus | betaine aldehyde + reduced coenzyme Q1 | - |
? | |
choline + phenazine methosulfate | - |
Rattus norvegicus | betaine aldehyde + reduced phenazine methosulfate | - |
r |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
45 | - |
- |
Rattus norvegicus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
9 | - |
- |
Rattus norvegicus |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
coenzyme Q | primary electron acceptor in vivo | Rattus norvegicus |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
1 | - |
2-Dimethylaminoethanol | inhibits the enzyme competitively | Rattus norvegicus | |
3.3 | - |
Betaine aldehyde | inhibits the oxidation of choline competitively | Rattus norvegicus |