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Literature summary for 1.1.98.6 extracted from
- Characterization of components of the anaerobic ribonucleotide reductase system from Escherichia coli (1992), J. Biol. Chem., 267, 25541-25547.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| ATP | strong stimulation | Escherichia coli |  |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| bathophenanthroline | 0.02 mM, 97% inhibition | Escherichia coli | |
| Co2+ | 0.02 mM, 75% inhibition | Escherichia coli | |
| desferal | 0.02 mM, 96% inhibition | Escherichia coli | |
| EDTA | 0.1 mM, 96% inhibition | Escherichia coli | |
| ferrozin | 0.1 mM, 72% inhibition | Escherichia coli | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 77000 | - |
2 * 77000, SDS-PAGE | Escherichia coli |
| 145000 | - |
gel filtration | Escherichia coli |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Escherichia coli | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Escherichia coli |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 260 | - |
pH 8.5, 22°C | Escherichia coli |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ribonucleoside 5'-triphosphate + formate | - |
Escherichia coli | 2'-deoxyribonucleoside 5'-triphosphate + CO2 + H2O | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| dimer | 2 * 77000, SDS-PAGE | Escherichia coli |
| More | occurence of a degradation product named beta2 of 74000 Da | Escherichia coli |
General Information
| General Information | Comment | Organism |
|---|---|---|
| physiological function | the enzyme is the actual ribonucleoside triphosphate reductase in the oxygen-sensitive ribonucleoside triphosphate reductase system. The reaction requires an auxiliary protein dA1 of 29000 Da, S-adenosylmethionine, NADPH (with NADH as a less active substitute), dithinthreitol, and magnesium ions, and is strongly stimulated by ATP | Escherichia coli |
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