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Literature summary for 1.1.98.2 extracted from
- Expression, purification and crystallization of native and selenomethionine labeled Mycobacterium tuberculosis FGD1 (Rv0407) using a Mycobacterium smegmatis expression system (2007), Protein Expr. Purif., 54, 38-44.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| initial attempts to produce recombinant FGD1 using Escherichia coli as a host is unsuccessful, but when the alternative host Mycobacterium smegmatis is used, soluble protein yields of 7 mg/l of culture are achieved | Mycobacterium tuberculosis |
| recombinant expression of His-tagged wild-type and selenomethionine-labeled FGD1 in Mycobacterium smegmatis strain mc24517 | Mycobacterium tuberculosis |
Crystallization (Commentary)
| Crystallization (Comment) | Organism |
|---|---|
| recombinant wild-type and selenomethionine-labeled FGD1, sitting drop vapour diffusion, mixing of protein solution in a 1:1 ratio with precipitant solution containing 1.6 M tri-sodium citrate, pH 6.5, microseeding, X-ray diffraction structure determination and analysis at 2.1 A resolution, multiwavelength anomalous diffraction | Mycobacterium tuberculosis |
| sitting drop vapour diffusion method, native and selenomethionine-labeled FGD1 are successfully crystallized by vapor diffusion, with the crystals diffracting to 2.1 A resolution | Mycobacterium tuberculosis |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| D-glucose 6-phosphate + oxidized coenzyme F420 | Mycobacterium tuberculosis | - |
D-glucono-1,5-lactone 6-phosphate + reduced coenzyme F420 | - |
? |  |
| D-glucose 6-phosphate + oxidized coenzyme F420 | Mycobacterium tuberculosis H37Rv | - |
D-glucono-1,5-lactone 6-phosphate + reduced coenzyme F420 | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Mycobacterium tuberculosis | - |
- |
- |
| Mycobacterium tuberculosis | - |
gene Rv0407 | - |
| Mycobacterium tuberculosis H37Rv | - |
gene Rv0407 | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Mycobacterium tuberculosis |
| recombinant His-tagged wild-type and selenomethionine-labeled FGD1 from Mycobacterium smegmatis strain mc24517 by nickel affinity chromatography and gel filtration | Mycobacterium tuberculosis |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| D-glucose 6-phosphate + oxidized coenzyme F420 | - |
Mycobacterium tuberculosis | D-glucono-1,5-lactone 6-phosphate + reduced coenzyme F420 | - |
? | |
| D-glucose 6-phosphate + oxidized coenzyme F420 | - |
Mycobacterium tuberculosis H37Rv | D-glucono-1,5-lactone 6-phosphate + reduced coenzyme F420 | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| F420-dependent glucose-6-phosphate dehydrogenase | - |
Mycobacterium tuberculosis |
| FGD1 | - |
Mycobacterium tuberculosis |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| coenzyme F420 | - |
Mycobacterium tuberculosis | |
General Information
| General Information | Comment | Organism |
|---|---|---|
| physiological function | FGD1 is an F420-dependent glucose-6-phosphate dehydrogenase essential for activation of the anti-TB compound PA-824 | Mycobacterium tuberculosis |
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