BRENDA - Enzyme Database
show all sequences of 1.1.98.2

Purification of a novel coenzyme F420-dependent glucose-6-phosphate dehydrogenase from Mycobacterium smegmatis

Purwantini, E.; Daniels, L.; J. Bacteriol. 178, 2861-2866 (1996)

Data extracted from this reference:

Inhibitors
Inhibitors
Commentary
Organism
Structure
additional information
no inhibition: D-fructose-1-phosphate, D-galactose-1-phosphate, D-galactose-6-phosphate, L-fucose-1-phosphate, D-glucose-1-phosphate, D-ribose-5-phosphate, D-ribose-1-phosphate, D-arabinose-5-phosphate, glyceraldehyde-3-phosphate, and glycerol-3-phosphate
Mycolicibacterium smegmatis
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.004
-
oxidized coenzyme F420
pH 7.0, 40°C
Mycolicibacterium smegmatis
1.6
-
D-glucose 6-phosphate
pH 7, 40°C
Mycolicibacterium smegmatis
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
40000
-
2 * 40000, SDS-PAGE
Mycolicibacterium smegmatis
78000
-
gel filtration
Mycolicibacterium smegmatis
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
D-glucose 6-phosphate + oxidized coenzyme F420
Mycolicibacterium smegmatis
there are to separate glucose-6-phosphate dehydrogenase activities in Mycobacterium smegmatis: coenzyme F420-dependent glucose-6-phosphate dehydrogenase and NADP+-dependent glucose-6-phosphate dehydrogenase (EC 1.1.1.49)
D-glucono-1,5-lactone 6-phosphate + reduced coenzyme F420
-
-
?
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Mycolicibacterium smegmatis
-
-
-
Purification (Commentary)
Commentary
Organism
ammonium sulfate fractionation, phenyl-Sepharose column chromatography, F420–ether-linked aminohexyl–Sepharose 4B affinity chromatography, and quaternary aminoethyl-Sephadex column chromatography
Mycolicibacterium smegmatis
Specific Activity [micromol/min/mg]
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
50.9
-
-
Mycolicibacterium smegmatis
Storage Stability
Storage Stability
Organism
4°C, 16 days, highly purified enzyme, retention of 60% and 20% of the original activity with and without glycerol, respectively
Mycolicibacterium smegmatis
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
D-fructose-6-phosphate + oxidized coenzyme F420
9% of the activity with 2 mM D-glucose 6-phosphate
698547
Mycolicibacterium smegmatis
?
-
-
-
?
D-glucose 6-phosphate + oxidized coenzyme F420
there are to separate glucose-6-phosphate dehydrogenase activities in Mycobacterium smegmatis: coenzyme F420-dependent glucose-6-phosphate dehydrogenase and NADP+-dependent glucose-6-phosphate dehydrogenase (EC 1.1.1.49)
698547
Mycolicibacterium smegmatis
D-glucono-1,5-lactone 6-phosphate + reduced coenzyme F420
-
-
-
?
D-glucose 6-phosphate + oxidized coenzyme F420
the purified enzyme used F420 as its obligate electron acceptor when D-glucose 6-phosphate is the substrate. NAD, NADP, flavin adenine dinucleotide, and flavin mononucleotide show no reactivity. The enzyme is very specific for D-glucose 6-phosphate. At pH 7.0 and 22°C, 10 mM D-mannose-6-phosphate and D-fructose-6-phosphate show 5% and 9%, respectively, of the activity with 2 mM D-glucose 6-phosphate. No activity with: D-fructose-1-phosphate, D-galactose-1-phosphate, D-galactose-6-phosphate, L-fucose-1-phosphate, D-glucose-1-phosphate, D-ribose-5-phosphate, D-ribose-1-phosphate, D-arabinose-5-phosphate, glyceraldehyde-3-phosphate, and glycerol-3-phosphate
698547
Mycolicibacterium smegmatis
D-glucono-1,5-lactone 6-phosphate + reduced coenzyme F420
-
-
-
?
D-mannose-6-phosphate + oxidized coenzyme F420
5% of the activity with 2 mM D-glucose 6-phosphate
698547
Mycolicibacterium smegmatis
?
-
-
-
?
Subunits
Subunits
Commentary
Organism
dimer
2 * 40000, SDS-PAGE
Mycolicibacterium smegmatis
Temperature Optimum [°C]
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
60
-
-
Mycolicibacterium smegmatis
Temperature Stability [°C]
Temperature Stability Minimum [°C]
Temperature Stability Maximum [°C]
Commentary
Organism
50
-
denaturation above
Mycolicibacterium smegmatis
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
5.5
-
maxima at pH 5.5 and pH 8.0
Mycolicibacterium smegmatis
8
-
maxima at pH 5.5 and pH 8.0
Mycolicibacterium smegmatis
pH Range
pH Minimum
pH Maximum
Commentary
Organism
5
8.5
pH 5: about 60% of maximal activity, pH 5.5: maximum, pH 7: about 50% of maximal activity, pH 8.0: maximal activity, pH 8.5: about 60% of maximal activity
Mycolicibacterium smegmatis
Cofactor
Cofactor
Commentary
Organism
Structure
coenzyme F420
-
Mycolicibacterium smegmatis
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
coenzyme F420
-
Mycolicibacterium smegmatis
Inhibitors (protein specific)
Inhibitors
Commentary
Organism
Structure
additional information
no inhibition: D-fructose-1-phosphate, D-galactose-1-phosphate, D-galactose-6-phosphate, L-fucose-1-phosphate, D-glucose-1-phosphate, D-ribose-5-phosphate, D-ribose-1-phosphate, D-arabinose-5-phosphate, glyceraldehyde-3-phosphate, and glycerol-3-phosphate
Mycolicibacterium smegmatis
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.004
-
oxidized coenzyme F420
pH 7.0, 40°C
Mycolicibacterium smegmatis
1.6
-
D-glucose 6-phosphate
pH 7, 40°C
Mycolicibacterium smegmatis
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
40000
-
2 * 40000, SDS-PAGE
Mycolicibacterium smegmatis
78000
-
gel filtration
Mycolicibacterium smegmatis
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
D-glucose 6-phosphate + oxidized coenzyme F420
Mycolicibacterium smegmatis
there are to separate glucose-6-phosphate dehydrogenase activities in Mycobacterium smegmatis: coenzyme F420-dependent glucose-6-phosphate dehydrogenase and NADP+-dependent glucose-6-phosphate dehydrogenase (EC 1.1.1.49)
D-glucono-1,5-lactone 6-phosphate + reduced coenzyme F420
-
-
?
Purification (Commentary) (protein specific)
Commentary
Organism
ammonium sulfate fractionation, phenyl-Sepharose column chromatography, F420–ether-linked aminohexyl–Sepharose 4B affinity chromatography, and quaternary aminoethyl-Sephadex column chromatography
Mycolicibacterium smegmatis
Specific Activity [micromol/min/mg] (protein specific)
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
50.9
-
-
Mycolicibacterium smegmatis
Storage Stability (protein specific)
Storage Stability
Organism
4°C, 16 days, highly purified enzyme, retention of 60% and 20% of the original activity with and without glycerol, respectively
Mycolicibacterium smegmatis
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
D-fructose-6-phosphate + oxidized coenzyme F420
9% of the activity with 2 mM D-glucose 6-phosphate
698547
Mycolicibacterium smegmatis
?
-
-
-
?
D-glucose 6-phosphate + oxidized coenzyme F420
there are to separate glucose-6-phosphate dehydrogenase activities in Mycobacterium smegmatis: coenzyme F420-dependent glucose-6-phosphate dehydrogenase and NADP+-dependent glucose-6-phosphate dehydrogenase (EC 1.1.1.49)
698547
Mycolicibacterium smegmatis
D-glucono-1,5-lactone 6-phosphate + reduced coenzyme F420
-
-
-
?
D-glucose 6-phosphate + oxidized coenzyme F420
the purified enzyme used F420 as its obligate electron acceptor when D-glucose 6-phosphate is the substrate. NAD, NADP, flavin adenine dinucleotide, and flavin mononucleotide show no reactivity. The enzyme is very specific for D-glucose 6-phosphate. At pH 7.0 and 22°C, 10 mM D-mannose-6-phosphate and D-fructose-6-phosphate show 5% and 9%, respectively, of the activity with 2 mM D-glucose 6-phosphate. No activity with: D-fructose-1-phosphate, D-galactose-1-phosphate, D-galactose-6-phosphate, L-fucose-1-phosphate, D-glucose-1-phosphate, D-ribose-5-phosphate, D-ribose-1-phosphate, D-arabinose-5-phosphate, glyceraldehyde-3-phosphate, and glycerol-3-phosphate
698547
Mycolicibacterium smegmatis
D-glucono-1,5-lactone 6-phosphate + reduced coenzyme F420
-
-
-
?
D-mannose-6-phosphate + oxidized coenzyme F420
5% of the activity with 2 mM D-glucose 6-phosphate
698547
Mycolicibacterium smegmatis
?
-
-
-
?
Subunits (protein specific)
Subunits
Commentary
Organism
dimer
2 * 40000, SDS-PAGE
Mycolicibacterium smegmatis
Temperature Optimum [°C] (protein specific)
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
60
-
-
Mycolicibacterium smegmatis
Temperature Stability [°C] (protein specific)
Temperature Stability Minimum [°C]
Temperature Stability Maximum [°C]
Commentary
Organism
50
-
denaturation above
Mycolicibacterium smegmatis
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
5.5
-
maxima at pH 5.5 and pH 8.0
Mycolicibacterium smegmatis
8
-
maxima at pH 5.5 and pH 8.0
Mycolicibacterium smegmatis
pH Range (protein specific)
pH Minimum
pH Maximum
Commentary
Organism
5
8.5
pH 5: about 60% of maximal activity, pH 5.5: maximum, pH 7: about 50% of maximal activity, pH 8.0: maximal activity, pH 8.5: about 60% of maximal activity
Mycolicibacterium smegmatis
Other publictions for EC 1.1.98.2
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
739925
Nguyen
Discovery and characterization ...
Rhodococcus jostii
Appl. Microbiol. Biotechnol.
101
2831-2842
2016
-
-
1
-
3
-
-
4
-
-
-
1
-
1
-
-
1
-
-
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1
6
-
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1
4
1
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2
-
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1
2
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3
-
-
-
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4
-
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-
1
-
-
-
1
-
-
-
1
6
-
-
-
1
4
1
-
-
-
-
-
-
-
4
4
740083
Oyugi
Investigating the reaction mec ...
Mycobacterium tuberculosis
Biochemistry
55
5566-5577
2016
-
-
1
-
5
-
-
12
-
-
-
1
-
3
-
-
1
-
-
-
-
-
1
-
-
-
-
12
1
-
-
1
-
-
-
-
-
1
1
-
5
-
-
-
-
12
-
-
-
1
-
-
-
1
-
-
-
-
1
-
-
-
-
12
1
-
-
-
-
-
-
-
11
11
723589
Bashiri
Tat-dependent translocation of ...
Mycobacterium tuberculosis, Mycobacterium tuberculosis H37Rv
PLoS ONE
7
e45003
2012
-
-
1
-
-
-
-
-
-
-
2
2
-
160
-
-
-
-
-
-
-
-
2
1
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-
1
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1
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1
1
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-
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-
2
2
-
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-
-
-
-
-
-
2
1
-
-
-
-
1
-
-
-
-
1
1
-
-
-
699008
Hasan
Glucose-6-phosphate accumulati ...
Mycolicibacterium smegmatis
J. Biol. Chem.
285
19135-19144
2010
-
-
-
-
-
-
-
-
-
-
-
1
-
6
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
698764
Bashiri
Crystal structures of F420-dep ...
Mycobacterium tuberculosis, Mycobacterium tuberculosis H37Rv
J. Biol. Chem.
283
17531-17541
2008
-
-
1
1
-
-
1
-
-
-
-
-
-
5
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
1
-
1
-
-
1
1
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
701052
Bashiri
Expression, purification and c ...
Mycobacterium tuberculosis, Mycobacterium tuberculosis H37Rv
Protein Expr. Purif.
54
38-44
2007
-
-
1
1
-
-
-
-
-
-
-
2
-
160
-
-
1
-
-
-
-
-
2
-
-
-
-
-
-
-
-
1
-
-
-
-
-
1
1
1
-
-
-
-
-
-
-
-
-
2
-
-
-
1
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
1
1
-
-
-
700931
Manjunatha
Identification of a nitroimida ...
Mycobacterium tuberculosis
Proc. Natl. Acad. Sci. USA
103
431-436
2006
-
1
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
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-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
698551
Purwantini
Molecular analysis of the gene ...
Mycolicibacterium smegmatis
J. Bacteriol.
180
2212-2219
1998
-
-
1
-
-
-
-
-
-
-
-
-
-
6
-
-
-
-
-
-
-
-
-
-
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-
-
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-
-
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1
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-
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-
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-
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-
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-
697967
Purwantini
Presence of F420-dependent glu ...
Gordonia amarae, Mycobacterium avium, Mycobacterium gordonae, Mycobacterium kansasii, Mycobacterium leprae, Mycobacterium scrofulaceum, Mycobacterium tuberculosis, Mycobacterium tuberculosis H37Ra / ATCC 25177, Mycobacterium tuberculosis H37Rv, Mycolicibacterium fortuitum, Mycolicibacterium smegmatis, no activity in Actinomadura kijaniata, no activity in Corynebacterium ammoniagenes, no activity in Corynebacterium glutamicum, no activity in Corynebacterium matruchotii, no activity in Methanobacterium thermoautotrophicum, no activity in Methanobacterium thermoautotrophicum Marburg / DSM 2133, no activity in Methanosarcina barkeri, no activity in Methanosarcina barkeri Fusaro, no activity in Methanospirillum hungateii, no activity in Streptomyces achromogenes, no activity in Streptomyces aureofaciens, no activity in Streptomyces avermitilis, no activity in Streptomyces lincolnensis, no activity in Streptomyces lividans, no activity in Streptomyces rimosus, Nocardia asteroides, Nocardia brasiliensis, Nocardia otitidiscaviarum, Nocardia tartaricans
FEMS Microbiol. Lett.
146
129-134
1997
-
-
-
-
-
-
-
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-
1
-
33
-
203
-
-
-
-
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-
14
-
33
-
13
-
-
-
13
-
-
13
-
-
-
-
-
-
13
-
-
-
-
-
-
-
-
1
-
33
-
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-
-
-
14
-
33
-
13
-
-
-
13
-
-
-
-
-
-
-
-
-
698547
Purwantini
Purification of a novel coenzy ...
Mycolicibacterium smegmatis
J. Bacteriol.
178
2861-2866
1996
-
-
-
-
-
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1
2
-
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2
1
-
4
-
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1
-
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-
1
1
4
1
1
-
1
-
2
1
-
1
-
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-
1
-
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1
-
2
-
-
2
1
-
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-
1
-
-
1
1
4
1
1
-
1
-
2
1
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-
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