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Literature summary for 1.1.98.2 extracted from
- Purification of a novel coenzyme F420-dependent glucose-6-phosphate dehydrogenase from Mycobacterium smegmatis (1996), J. Bacteriol., 178, 2861-2866.
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| additional information | no inhibition: D-fructose-1-phosphate, D-galactose-1-phosphate, D-galactose-6-phosphate, L-fucose-1-phosphate, D-glucose-1-phosphate, D-ribose-5-phosphate, D-ribose-1-phosphate, D-arabinose-5-phosphate, glyceraldehyde-3-phosphate, and glycerol-3-phosphate | Mycolicibacterium smegmatis |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.004 | - |
oxidized coenzyme F420 | pH 7.0, 40°C | Mycolicibacterium smegmatis |  |
| 1.6 | - |
D-glucose 6-phosphate | pH 7, 40°C | Mycolicibacterium smegmatis | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 40000 | - |
2 * 40000, SDS-PAGE | Mycolicibacterium smegmatis |
| 78000 | - |
gel filtration | Mycolicibacterium smegmatis |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| D-glucose 6-phosphate + oxidized coenzyme F420 | Mycolicibacterium smegmatis | there are to separate glucose-6-phosphate dehydrogenase activities in Mycobacterium smegmatis: coenzyme F420-dependent glucose-6-phosphate dehydrogenase and NADP+-dependent glucose-6-phosphate dehydrogenase (EC 1.1.1.49) | D-glucono-1,5-lactone 6-phosphate + reduced coenzyme F420 | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Mycolicibacterium smegmatis | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| ammonium sulfate fractionation, phenyl-Sepharose column chromatography, F420ether-linked aminohexylSepharose 4B affinity chromatography, and quaternary aminoethyl-Sephadex column chromatography | Mycolicibacterium smegmatis |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 50.9 | - |
- |
Mycolicibacterium smegmatis |
Storage Stability
| Storage Stability | Organism |
|---|---|
| 4°C, 16 days, highly purified enzyme, retention of 60% and 20% of the original activity with and without glycerol, respectively | Mycolicibacterium smegmatis |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| D-fructose-6-phosphate + oxidized coenzyme F420 | 9% of the activity with 2 mM D-glucose 6-phosphate | Mycolicibacterium smegmatis | ? | - |
? | |
| D-glucose 6-phosphate + oxidized coenzyme F420 | there are to separate glucose-6-phosphate dehydrogenase activities in Mycobacterium smegmatis: coenzyme F420-dependent glucose-6-phosphate dehydrogenase and NADP+-dependent glucose-6-phosphate dehydrogenase (EC 1.1.1.49) | Mycolicibacterium smegmatis | D-glucono-1,5-lactone 6-phosphate + reduced coenzyme F420 | - |
? | |
| D-glucose 6-phosphate + oxidized coenzyme F420 | the purified enzyme used F420 as its obligate electron acceptor when D-glucose 6-phosphate is the substrate. NAD, NADP, flavin adenine dinucleotide, and flavin mononucleotide show no reactivity. The enzyme is very specific for D-glucose 6-phosphate. At pH 7.0 and 22°C, 10 mM D-mannose-6-phosphate and D-fructose-6-phosphate show 5% and 9%, respectively, of the activity with 2 mM D-glucose 6-phosphate. No activity with: D-fructose-1-phosphate, D-galactose-1-phosphate, D-galactose-6-phosphate, L-fucose-1-phosphate, D-glucose-1-phosphate, D-ribose-5-phosphate, D-ribose-1-phosphate, D-arabinose-5-phosphate, glyceraldehyde-3-phosphate, and glycerol-3-phosphate | Mycolicibacterium smegmatis | D-glucono-1,5-lactone 6-phosphate + reduced coenzyme F420 | - |
? | |
| D-mannose-6-phosphate + oxidized coenzyme F420 | 5% of the activity with 2 mM D-glucose 6-phosphate | Mycolicibacterium smegmatis | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| dimer | 2 * 40000, SDS-PAGE | Mycolicibacterium smegmatis |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| coenzyme F420-dependent glucose-6-phosphate dehydrogenase | - |
Mycolicibacterium smegmatis |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 60 | - |
- |
Mycolicibacterium smegmatis |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 50 | - |
denaturation above | Mycolicibacterium smegmatis |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 5.5 | - |
maxima at pH 5.5 and pH 8.0 | Mycolicibacterium smegmatis |
| 8 | - |
maxima at pH 5.5 and pH 8.0 | Mycolicibacterium smegmatis |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 5 | 8.5 | pH 5: about 60% of maximal activity, pH 5.5: maximum, pH 7: about 50% of maximal activity, pH 8.0: maximal activity, pH 8.5: about 60% of maximal activity | Mycolicibacterium smegmatis |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| coenzyme F420 | - |
Mycolicibacterium smegmatis | |
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