Application | Comment | Organism |
---|---|---|
biotechnology | the PQQ-dependent lactate dehydrogenase from Gluconobacter is capable of direct electron transfer at carbon and gold electrodes, which makes it useful for application in biosensors or biofuel cells, overview | Gluconobacter oxydans |
biotechnology | the PQQ-dependent lactate dehydrogenase from Gluconobacter is capable of direct electron transfer at carbon and gold electrodes, which makes it useful for application in biosensors or biofuel cells, overview | Gluconobacter sp. |
Protein Variants | Comment | Organism |
---|---|---|
additional information | bioanode fabrication using the partially purified enzyme immobilized on a membrane, usage of carbon fiber paper, overview | Gluconobacter sp. |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Gluconobacter oxydans | - |
ATCC 621 | - |
Gluconobacter sp. | - |
strain 33 | - |
Gluconobacter sp. DSM 3504 / ATCC 15163 | - |
strain 33 | - |
Purification (Comment) | Organism |
---|---|
native enzyme from membranes by solubilization with Triton X-100 and anion exchange chromatography | Gluconobacter sp. |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-lactate + 2,6-dichlorophenol indophenol | reduction of the acceptor 2,6-dichlorophenol indophenol is mediated by phenazine methosulfate | Gluconobacter oxydans | pyruvate + reduced 2,6-dichlorophenol indophenol | - |
? | |
L-lactate + 2,6-dichlorophenol indophenol | reduction of the acceptor 2,6-dichlorophenol indophenol is mediated by phenazine methosulfate | Gluconobacter sp. | pyruvate + reduced 2,6-dichlorophenol indophenol | - |
? | |
L-lactate + 2,6-dichlorophenol indophenol | reduction of the acceptor 2,6-dichlorophenol indophenol is mediated by phenazine methosulfate | Gluconobacter sp. DSM 3504 / ATCC 15163 | pyruvate + reduced 2,6-dichlorophenol indophenol | - |
? |
Synonyms | Comment | Organism |
---|---|---|
PQQ-dependent lactate dehydrogenase | - |
Gluconobacter oxydans |
PQQ-dependent lactate dehydrogenase | - |
Gluconobacter sp. |
PQQ-LDH | - |
Gluconobacter oxydans |
PQQ-LDH | - |
Gluconobacter sp. |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Gluconobacter oxydans |
37 | - |
assay at | Gluconobacter sp. |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.3 | - |
assay at | Gluconobacter oxydans |
7.3 | - |
assay at | Gluconobacter sp. |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
2,6-dichlorophenol indophenol | - |
Gluconobacter oxydans | |
2,6-dichlorophenol indophenol | - |
Gluconobacter sp. | |
phenazine methosulfate | mediates reduction of 2,6-dichlorophenol-indophenol | Gluconobacter oxydans | |
phenazine methosulfate | mediates reduction of 2,6-dichlorophenol-indophenol | Gluconobacter sp. | |
pyrroloquinoline quinone | dependent on | Gluconobacter oxydans | |
pyrroloquinoline quinone | dependent on | Gluconobacter sp. |