Activating Compound | Comment | Organism | Structure |
---|---|---|---|
additional information | the enzyme expression is induced by a temperature shift and by growth on L-lactate | Corynebacterium glutamicum |
Cloned (Comment) | Organism |
---|---|
gene ldhD, DNA microarray expression analysis, gene ldhD acts in an operon with the permease gene NCgl2816 | Corynebacterium glutamicum |
Protein Variants | Comment | Organism |
---|---|---|
additional information | disruption of the ldhD-NCgl2816 operon results in loss of the ability to utilize L-lactate as sole carbon source, the ability can be restored by expression of gene ldhD, overview | Corynebacterium glutamicum |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.51 | - |
L-lactate | pH 7.5, 30°C | Corynebacterium glutamicum |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Corynebacterium glutamicum | - |
strain ATCC 13032, gene lldD | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.003 | - |
wild-type strain ATCC 13032, without L-lactate | Corynebacterium glutamicum |
0.23 | - |
wild-type strain ATCC 13032, with L-lactate | Corynebacterium glutamicum |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-lactate + oxidized 2,6-dichlorophenol indophenol | stereospecific for L-lactate | Corynebacterium glutamicum | pyruvate + reduced 2,6-dichlorophenol indophenol | - |
? |
Synonyms | Comment | Organism |
---|---|---|
LldD | - |
Corynebacterium glutamicum |
quinone-dependent L-lactate dehydrogenase | - |
Corynebacterium glutamicum |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
assay at | Corynebacterium glutamicum |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
assay at | Corynebacterium glutamicum |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
2,6-dichlorophenol indophenol | - |
Corynebacterium glutamicum | |
quinone | dependent on | Corynebacterium glutamicum |