Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli strain Origami2 (DE3) | Aspergillus flavus |
Crystallization (Comment) | Organism |
---|---|
in complex with FAD and/or D-glucono-1,5-lactone, sitting-drop vapor diffusion method, using 0.1 M of BisTris, pH 6.5, and 2225% (w/v) PEG3350 | Aspergillus flavus |
Protein Variants | Comment | Organism |
---|---|---|
H505A | the mutant enzyme shows drastic decrease in the enzymatic activity | Aspergillus flavus |
H548A | the mutant enzyme shows drastic decrease in the enzymatic activity | Aspergillus flavus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Aspergillus flavus | B8MX95 | - |
- |
Aspergillus flavus ATCC 200026 | B8MX95 | - |
- |
Purification (Comment) | Organism |
---|---|
resource Q column chromatography | Aspergillus flavus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
D-glucose + 2,6-dichlorophenolindophenol | - |
Aspergillus flavus | D-glucono-1,5-lactone + reduced 2,6-dichlorophenolindophenol | - |
? | |
D-glucose + 2,6-dichlorophenolindophenol | - |
Aspergillus flavus ATCC 200026 | D-glucono-1,5-lactone + reduced 2,6-dichlorophenolindophenol | - |
? |
Synonyms | Comment | Organism |
---|---|---|
FAD glucose dehydrogenase | - |
Aspergillus flavus |
FAD-dependent glucose dehydrogenase | - |
Aspergillus flavus |
FADGDH | - |
Aspergillus flavus |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
FAD | - |
Aspergillus flavus |