BRENDA - Enzyme Database
show all sequences of 1.1.5.9

Crystallographic analysis of FAD-dependent glucose dehydrogenase

Komori, H.; Inaka, K.; Furubayashi, N.; Honda, M.; Higuchi, Y.; Acta Crystallogr. Sect. F 71, 1017-1019 (2015)

Data extracted from this reference:

Cloned(Commentary)
Commentary
Organism
expressed in Aspergillus oryzae strain NS4
Aspergillus terreus
Crystallization (Commentary)
Crystallization
Organism
sitting drop vapor diffusion method, using 30% (w/v) PEG 8000, 0.2 M ammonium sulfate, 0.1 M sodium acetate pH 4.5
Aspergillus terreus
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
D-glucose + a quinone
Aspergillus terreus
-
D-glucono-1,5-lactone + a quinol
-
-
?
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Aspergillus terreus
-
-
-
Purification (Commentary)
Commentary
Organism
Toyopearl Butyl-650C column chromatography, DEAE Cellufine A-500m column chromatography, and Q-Sepharose column chromatography
Aspergillus terreus
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
D-glucose + a quinone
-
739817
Aspergillus terreus
D-glucono-1,5-lactone + a quinol
-
-
-
?
Cofactor
Cofactor
Commentary
Organism
Structure
FAD
-
Aspergillus terreus
Cloned(Commentary) (protein specific)
Commentary
Organism
expressed in Aspergillus oryzae strain NS4
Aspergillus terreus
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
FAD
-
Aspergillus terreus
Crystallization (Commentary) (protein specific)
Crystallization
Organism
sitting drop vapor diffusion method, using 30% (w/v) PEG 8000, 0.2 M ammonium sulfate, 0.1 M sodium acetate pH 4.5
Aspergillus terreus
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
D-glucose + a quinone
Aspergillus terreus
-
D-glucono-1,5-lactone + a quinol
-
-
?
Purification (Commentary) (protein specific)
Commentary
Organism
Toyopearl Butyl-650C column chromatography, DEAE Cellufine A-500m column chromatography, and Q-Sepharose column chromatography
Aspergillus terreus
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
D-glucose + a quinone
-
739817
Aspergillus terreus
D-glucono-1,5-lactone + a quinol
-
-
-
?
Other publictions for EC 1.1.5.9
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
739924
Ozawa
Identification and characteriz ...
Rasamsonia emersonii, Rasamsonia emersonii NBRC 31232, Thermoascus crustaceus, Thermoascus crustaceus NBRC 9129
Appl. Microbiol. Biotechnol.
101
173-183
2017
-
-
2
-
-
-
-
-
-
-
2
-
-
11
-
2
2
-
-
-
-
-
20
2
2
-
2
-
2
-
2
2
-
-
-
-
-
2
2
-
-
-
-
-
-
-
-
-
2
-
-
-
2
2
-
-
-
-
20
2
2
-
2
-
2
-
2
-
-
-
-
-
-
-
740195
Sode
Novel fungal FAD glucose dehyd ...
Aspergillus niger
Biosens. Bioelectron.
87
305-311
2017
-
-
1
-
-
-
-
2
-
-
1
-
-
4
-
-
-
-
1
-
-
-
3
1
-
-
-
-
-
-
-
1
-
-
-
-
-
1
1
-
-
-
-
-
-
2
-
-
1
-
-
-
-
-
1
-
-
-
3
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
741496
Iwasa
-
Thermophilic Talaromyces emer ...
Rasamsonia emersonii
ACS Omega
2
1660-1665
2017
-
1
1
-
-
-
-
-
-
-
-
-
-
1
-
1
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
1
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
741724
Ozawa
Identification and characteri ...
Rasamsonia emersonii, Thermoascus crustaceus, Thermoascus crustaceus NBRC 9129
Appl. Microbiol. Biotechnol.
101
173-183
2017
-
-
2
-
-
-
-
4
2
-
-
-
-
10
-
2
-
-
-
-
2
-
6
2
4
4
8
-
3
-
4
2
-
-
-
-
-
2
2
-
-
-
-
-
-
4
2
-
-
-
-
-
2
-
-
-
2
-
6
2
4
4
8
-
3
-
4
-
-
-
-
-
-
-
740120
Shiota
An Fe-S cluster in the conserv ...
Burkholderia cepacia
Bioelectrochemistry
112
178-183
2016
-
-
-
-
4
-
-
2
-
-
-
-
-
2
-
-
1
-
-
-
-
-
1
1
-
-
-
-
-
-
-
2
-
-
-
-
-
-
2
-
4
-
-
-
-
2
-
-
-
-
-
-
-
1
-
-
-
-
1
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
740363
Iwasa
-
Thermostable FAD-dependent glu ...
Thermoascus aurantiacus, Thermoascus aurantiacus NBRC 6766, Thermoascus aurantiacus NBRC 9748
Electrochemistry
84
342-348
2016
-
-
1
-
-
-
-
-
-
-
-
-
-
3
-
1
-
-
-
-
-
-
20
-
1
-
1
-
2
-
1
1
-
-
-
-
-
1
1
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
20
-
1
-
1
-
2
-
1
-
-
-
-
-
-
-
739817
Komori
Crystallographic analysis of F ...
Aspergillus terreus
Acta Crystallogr. Sect. F
71
1017-1019
2015
-
-
1
1
-
-
-
-
-
-
-
1
-
2
-
-
1
-
-
-
-
-
1
-
-
-
-
-
-
-
-
1
-
-
-
-
-
1
1
1
-
-
-
-
-
-
-
-
-
1
-
-
-
1
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
740224
Sakai
Stabilization of fungi-derived ...
Aspergillus flavus
Biotechnol. Lett.
37
1091-1099
2015
-
-
1
-
1
-
-
1
-
-
2
-
-
1
-
-
1
-
-
-
-
-
3
1
-
-
1
2
-
-
-
1
-
-
-
-
-
1
1
-
1
-
-
-
-
1
-
-
2
-
-
-
-
1
-
-
-
-
3
1
-
-
1
2
-
-
-
-
-
-
-
-
1
1
740778
Satake
Novel glucose dehydrogenase fr ...
Mucor circinelloides, Mucor circinelloides NISL0103
J. Biosci. Bioeng.
120
498-503
2015
-
-
1
-
-
-
-
1
-
-
-
-
-
4
-
1
1
-
-
1
-
-
14
1
1
1
1
1
1
-
1
1
-
-
-
-
-
1
1
-
-
-
-
-
-
1
-
-
-
-
-
-
1
1
-
1
-
-
14
1
1
1
1
1
1
-
1
-
-
-
-
-
-
-
741412
Yoshida
Structural analysis of fungus- ...
Aspergillus flavus, Aspergillus flavus ATCC 200026
Sci. Rep.
5
13498
2015
-
-
1
1
2
-
-
-
-
-
-
-
-
4
-
-
1
-
-
-
-
-
2
-
-
-
-
-
-
-
-
1
-
-
-
-
-
1
1
1
2
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
740853
Yang
Efficient expression, purifica ...
Aspergillus terreus, Aspergillus terreus NIH2624
J. Microbiol. Biotechnol.
24
1516-1524
2014
-
-
1
-
-
-
-
1
-
-
-
-
-
7
-
-
1
-
-
-
-
-
13
1
1
1
1
-
1
1
1
1
-
-
-
-
-
1
1
-
-
-
-
-
-
1
-
-
-
-
-
-
-
1
-
-
-
-
13
1
1
1
1
-
1
1
1
-
-
-
-
-
-
-
726109
Fapyane
High performance enzyme fuel c ...
Burkholderia cepacia
Phys. Chem. Chem. Phys.
15
9508-9512
2013
-
-
1
-
1
-
-
1
-
-
2
1
-
4
-
-
1
-
-
-
-
-
3
1
-
-
-
1
-
-
-
1
-
-
-
-
-
1
1
-
1
-
-
-
-
1
-
-
2
1
-
-
-
1
-
-
-
-
3
1
-
-
-
1
-
-
-
-
-
-
-
-
-
-
740387
Yamashita
Direct electron transfer type ...
Burkholderia cepacia
Enzyme Microb. Technol.
52
123-128
2013
-
-
1
-
38
-
-
-
-
-
-
-
-
3
-
-
1
-
-
-
-
-
2
-
-
-
-
-
-
-
-
1
-
-
-
-
-
1
1
-
38
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
723928
Zafar
Characterization of different ...
Aspergillus sp., Colletotrichum gloeosporioides, Komagataella pastoris
Anal. Bioanal. Chem.
402
2069-2077
2012
-
3
1
-
3
-
-
-
-
-
4
3
-
6
-
3
-
-
-
1
3
1
8
1
-
-
-
-
-
-
-
3
-
-
-
-
3
1
3
-
3
-
-
-
-
-
-
-
4
3
-
-
3
-
-
1
3
1
8
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
723944
Zafar
Electron-transfer studies with ...
Colletotrichum gloeosporioides
Anal. Chem.
84
334-341
2012
-
-
1
-
1
-
-
-
1
-
-
1
-
3
-
-
1
-
-
-
-
-
1
-
1
-
-
-
1
-
-
1
-
-
-
-
-
1
1
-
1
-
-
-
-
-
1
-
-
1
-
-
-
1
-
-
-
-
1
-
1
-
-
-
1
-
-
-
-
-
-
-
-
-
724876
Monosik
Amperometric glucose biosensor ...
Aspergillus oryzae
Enzyme Microb. Technol.
50
227-232
2012
-
2
-
-
1
-
-
1
-
-
-
1
-
4
-
-
-
-
-
-
-
-
1
-
1
-
-
-
1
1
-
1
-
-
-
-
2
-
1
-
1
-
-
-
-
1
-
-
-
1
-
-
-
-
-
-
-
-
1
-
1
-
-
-
1
1
-
-
-
1
1
-
-
-
724639
Mori
Screening of Aspergillus-deriv ...
Aspergillus oryzae, Aspergillus terreus
Biotechnol. Lett.
33
2255-2263
2011
-
-
2
-
-
-
-
-
-
-
-
2
-
2
-
-
-
-
-
-
-
-
8
-
-
-
-
-
-
-
-
2
-
-
-
-
-
2
2
-
-
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
8
-
-
-
-
-
-
-
-
-
-
2
2
-
-
-
725879
Sygmund
Heterologous overexpression of ...
Colletotrichum gloeosporioides
Microb. Cell Fact.
10
106
2011
-
-
1
-
-
-
-
8
-
-
1
1
-
6
-
1
1
-
-
-
1
-
3
1
1
-
1
8
1
-
1
1
-
-
-
-
-
1
1
-
-
-
-
-
-
8
-
-
1
1
-
-
1
1
-
-
1
-
3
1
1
-
1
8
1
-
1
-
-
-
-
-
8
8
725906
Sygmund
Reduction of quinones and phen ...
Colletotrichum gloeosporioides, Colletotrichum gloeosporioides DSM 62728
Microbiology
157
3203-3212
2011
-
-
1
-
-
-
-
-
1
-
2
2
-
7
-
1
1
-
-
-
2
-
11
1
1
-
-
-
2
-
-
1
-
1
-
-
-
1
1
-
-
-
-
-
-
-
1
-
2
2
-
-
1
1
-
-
2
-
11
1
1
-
-
-
2
-
-
1
-
2
2
-
-
-
685757
Yamaoka
Site directed mutagenesis stud ...
Burkholderia cepacia
Biotechnol. Lett.
30
1967-1972
2008
-
1
1
-
38
-
-
4
-
-
-
-
-
3
-
-
1
-
-
-
9
-
4
-
-
-
-
-
-
-
-
1
-
-
-
-
1
1
1
-
38
-
-
-
-
4
-
-
-
-
-
-
-
1
-
-
9
-
4
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
696897
Okuda-Shimazaki
Biofuel cell system employing ...
Burkholderia cepacia, Burkholderia cepacia SM4
Biotechnol. Lett.
30
1753-1758
2008
-
1
1
-
-
-
-
-
1
-
-
-
-
4
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
1
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
668023
Tsujimura
Novel FAD-dependent glucose de ...
Aspergillus terreus
Biosci. Biotechnol. Biochem.
70
654-659
2006
-
-
-
-
-
-
1
1
-
2
-
-
-
2
-
-
-
-
-
-
-
-
1
-
1
1
-
1
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
1
-
2
-
-
-
-
-
-
-
-
-
-
1
-
1
1
-
1
-
1
-
-
-
-
-
-
-
-
696891
Yamaoka
Essential role of the small su ...
Burkholderia cepacia, Burkholderia cepacia SM4
Biotechnol. Lett.
26
1757-1761
2004
-
-
1
-
-
-
-
-
-
-
4
-
-
5
-
-
1
-
-
-
-
-
4
1
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
4
-
-
-
-
1
-
-
-
-
4
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
348279
Lovallo
Alteration in FAD-glucose dehy ...
Manduca sexta
J. Insect Physiol.
45
1037-1048
1999
-
-
-
-
-
-
-
-
-
-
-
1
-
3
-
-
-
-
-
2
-
-
2
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
2
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
348280
Yamazaki
-
Increased thermal stability of ...
Bacteria
Biotechnol. Lett.
21
199-202
1999
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
1
-
2
2
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
2
2
1
-
-
-
-
-
-
-
-
-
-
-
695715
Yamazaki
-
Subunit Analyses of a Novel Th ...
Burkholderia cepacia
Appl. Biochem. Biotechnol.
77-79
325-335
1999
-
-
-
-
-
-
1
4
-
-
2
-
-
1
-
-
1
-
-
-
1
-
2
1
1
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
4
-
-
2
-
-
-
-
1
-
-
1
-
2
1
1
1
-
-
-
-
-
-
-
-
-
-
-
-
697670
Sode
-
A novel thermostable glucose d ...
Burkholderia cepacia
Enzyme Microb. Technol.
19
82-85
1996
-
-
-
-
-
-
-
-
1
-
5
-
-
1
-
-
1
-
-
-
3
-
1
2
2
2
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
5
-
-
-
-
1
-
-
3
-
1
2
2
2
2
-
-
-
-
-
-
-
-
-
-
-
348278
Cox-Foster
-
Induction of localization of F ...
Manduca sexta
J. Insect Physiol.
40
235-249
1994
-
1
-
-
-
-
-
-
-
-
-
1
-
1
-
-
-
-
-
1
-
-
1
-
-
-
-
-
-
-
-
1
-
-
-
-
1
-
1
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
1
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
348276
Matsushita
-
Gluconsäure bildende Enzyme be ...
Pseudomonas sp.
Agric. Biol. Chem.
44
1505-1512
1980
-
-
-
-
-
-
1
7
1
-
2
-
-
1
-
-
1
-
-
-
1
-
11
2
-
-
-
-
4
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
7
1
-
2
-
-
-
-
1
-
-
1
-
11
2
-
-
-
-
4
-
-
-
-
-
-
-
-
-
348275
Müller
Gluconic acid forming enzymes ...
Aspergillus niger
Zentralbl. Bakteriol. Parasitenkd. Infektionskr. Hyg.
132
14-24
1977
-
-
-
-
-
-
-
1
-
-
-
-
-
1
-
-
-
-
-
1
-
-
2
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
1
-
-
2
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
348274
Bak
Studies on glucose dehydrogena ...
Aspergillus oryzae
Biochim. Biophys. Acta
139
277-293
1967
-
-
-
-
-
1
-
-
-
-
2
-
-
1
-
1
1
-
-
2
1
1
1
-
-
-
3
-
-
-
2
1
-
-
-
-
-
-
1
-
-
1
-
-
-
-
-
-
2
-
-
-
1
1
-
2
1
1
1
-
-
-
3
-
-
-
2
-
-
-
-
-
-
-
348277
Hauge
Glucose dehydrogenase of Bacte ...
Acinetobacter baumannii
J. Biol. Chem.
239
3630-3639
1964
-
-
-
-
-
-
-
-
-
-
1
-
-
1
-
-
1
-
-
-
1
-
-
-
-
-
-
1
-
-
-
1
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
1
-
-
-
-
1
-
-
1
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-