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Literature summary for 1.1.5.9 extracted from
- High performance enzyme fuel cells using a genetically expressed FAD-dependent glucose dehydrogenase alpha-subunit of Burkholderia cepacia immobilized in a carbon nanotube electrode for low glucose conditions (2013), Phys. Chem. Chem. Phys., 15, 9508-9512.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expression in Escherichia coli strain BL21RIL, no GDH activity when cells expressing the alpha-subunit alone | Burkholderia cepacia |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| additional information | the purified recombinnat enzyme is used as an anode catalyst for enzyme fuel cells, method, overview. The recombinant FAD-GDH is able to maintain its native glucose affinity during immobilization in the carbon nanotube and operation of enzyme fuel cells. Heterogeneous electron transfer coefficient of FAD-GDHmenadione on a glassy carbon electrode was 10.73/s | Burkholderia cepacia |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.4638 | - |
D-glucose | pH and temperature not specified in the publication | Burkholderia cepacia |  |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 18000 | - |
1 * 59800 + 1 * 18000, SDS-PAGE | Burkholderia cepacia |
| 59800 | - |
1 * 59800 + 1 * 18000, SDS-PAGE | Burkholderia cepacia |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| D-glucose + a quinone | Burkholderia cepacia | - |
D-glucono-1,5-lactone + a quinol | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Burkholderia cepacia | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| recombinant enzyme from Escherichia coli strain BL21RIL | Burkholderia cepacia |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| D-glucose + a quinone | - |
Burkholderia cepacia | D-glucono-1,5-lactone + a quinol | - |
? | |
| D-glucose + menadione | - |
Burkholderia cepacia | D-glucono-1,5-lactone + menadiol | - |
? | |
| D-glucose + oxidized 2,6-dichlorophenol indophenol | - |
Burkholderia cepacia | D-glucono-1,5-lactone + reduced 2,6-dichlorophenol indophenol | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| dimer | 1 * 59800 + 1 * 18000, SDS-PAGE | Burkholderia cepacia |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| FAD-dependent glucose dehydrogenase | - |
Burkholderia cepacia |
| FAD-GDH | - |
Burkholderia cepacia |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 69.54 | - |
D-glucose | pH and temperature not specified in the publication | Burkholderia cepacia | |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| FAD | dependent on, bound in the catalytic site | Burkholderia cepacia | |
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Release 2023.1 (January 2023)
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