BRENDA - Enzyme Database
show all sequences of 1.1.5.9

High performance enzyme fuel cells using a genetically expressed FAD-dependent glucose dehydrogenase alpha-subunit of Burkholderia cepacia immobilized in a carbon nanotube electrode for low glucose conditions

Fapyane, D.; Lee, S.J.; Kang, S.H.; Lim, D.H.; Cho, K.K.; Nam, T.H.; Ahn, J.P.; Ahn, J.H.; Kim, S.W.; Chang, I.S.; Phys. Chem. Chem. Phys. 15, 9508-9512 (2013)

Data extracted from this reference:

Cloned(Commentary)
Commentary
Organism
expression in Escherichia coli strain BL21RIL, no GDH activity when cells expressing the alpha-subunit alone
Burkholderia cepacia
Engineering
Amino acid exchange
Commentary
Organism
additional information
the purified recombinnat enzyme is used as an anode catalyst for enzyme fuel cells, method, overview. The recombinant FAD-GDH is able to maintain its native glucose affinity during immobilization in the carbon nanotube and operation of enzyme fuel cells. Heterogeneous electron transfer coefficient of FAD-GDH–menadione on a glassy carbon electrode was 10.73/s
Burkholderia cepacia
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.4638
-
D-glucose
pH and temperature not specified in the publication
Burkholderia cepacia
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
18000
-
1 * 59800 + 1 * 18000, SDS-PAGE
Burkholderia cepacia
59800
-
1 * 59800 + 1 * 18000, SDS-PAGE
Burkholderia cepacia
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
D-glucose + a quinone
Burkholderia cepacia
-
D-glucono-1,5-lactone + a quinol
-
-
?
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Burkholderia cepacia
-
-
-
Purification (Commentary)
Commentary
Organism
recombinant enzyme from Escherichia coli strain BL21RIL
Burkholderia cepacia
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
D-glucose + a quinone
-
726109
Burkholderia cepacia
D-glucono-1,5-lactone + a quinol
-
-
-
?
D-glucose + menadione
-
726109
Burkholderia cepacia
D-glucono-1,5-lactone + menadiol
-
-
-
?
D-glucose + oxidized 2,6-dichlorophenol indophenol
-
726109
Burkholderia cepacia
D-glucono-1,5-lactone + reduced 2,6-dichlorophenol indophenol
-
-
-
?
Subunits
Subunits
Commentary
Organism
dimer
1 * 59800 + 1 * 18000, SDS-PAGE
Burkholderia cepacia
Turnover Number [1/s]
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
69.54
-
D-glucose
pH and temperature not specified in the publication
Burkholderia cepacia
Cofactor
Cofactor
Commentary
Organism
Structure
FAD
dependent on, bound in the catalytic site
Burkholderia cepacia
Cloned(Commentary) (protein specific)
Commentary
Organism
expression in Escherichia coli strain BL21RIL, no GDH activity when cells expressing the alpha-subunit alone
Burkholderia cepacia
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
FAD
dependent on, bound in the catalytic site
Burkholderia cepacia
Engineering (protein specific)
Amino acid exchange
Commentary
Organism
additional information
the purified recombinnat enzyme is used as an anode catalyst for enzyme fuel cells, method, overview. The recombinant FAD-GDH is able to maintain its native glucose affinity during immobilization in the carbon nanotube and operation of enzyme fuel cells. Heterogeneous electron transfer coefficient of FAD-GDH–menadione on a glassy carbon electrode was 10.73/s
Burkholderia cepacia
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.4638
-
D-glucose
pH and temperature not specified in the publication
Burkholderia cepacia
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
18000
-
1 * 59800 + 1 * 18000, SDS-PAGE
Burkholderia cepacia
59800
-
1 * 59800 + 1 * 18000, SDS-PAGE
Burkholderia cepacia
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
D-glucose + a quinone
Burkholderia cepacia
-
D-glucono-1,5-lactone + a quinol
-
-
?
Purification (Commentary) (protein specific)
Commentary
Organism
recombinant enzyme from Escherichia coli strain BL21RIL
Burkholderia cepacia
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
D-glucose + a quinone
-
726109
Burkholderia cepacia
D-glucono-1,5-lactone + a quinol
-
-
-
?
D-glucose + menadione
-
726109
Burkholderia cepacia
D-glucono-1,5-lactone + menadiol
-
-
-
?
D-glucose + oxidized 2,6-dichlorophenol indophenol
-
726109
Burkholderia cepacia
D-glucono-1,5-lactone + reduced 2,6-dichlorophenol indophenol
-
-
-
?
Subunits (protein specific)
Subunits
Commentary
Organism
dimer
1 * 59800 + 1 * 18000, SDS-PAGE
Burkholderia cepacia
Turnover Number [1/s] (protein specific)
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
69.54
-
D-glucose
pH and temperature not specified in the publication
Burkholderia cepacia
Other publictions for EC 1.1.5.9
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
739924
Ozawa
Identification and characteriz ...
Rasamsonia emersonii, Rasamsonia emersonii NBRC 31232, Thermoascus crustaceus, Thermoascus crustaceus NBRC 9129
Appl. Microbiol. Biotechnol.
101
173-183
2017
-
-
2
-
-
-
-
-
-
-
2
-
-
11
-
2
2
-
-
-
-
-
20
2
2
-
2
-
2
-
2
2
-
-
-
-
-
2
2
-
-
-
-
-
-
-
-
-
2
-
-
-
2
2
-
-
-
-
20
2
2
-
2
-
2
-
2
-
-
-
-
-
-
-
740195
Sode
Novel fungal FAD glucose dehyd ...
Aspergillus niger
Biosens. Bioelectron.
87
305-311
2017
-
-
1
-
-
-
-
2
-
-
1
-
-
4
-
-
-
-
1
-
-
-
3
1
-
-
-
-
-
-
-
1
-
-
-
-
-
1
1
-
-
-
-
-
-
2
-
-
1
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-
-
-
-
1
-
-
-
3
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
741496
Iwasa
-
Thermophilic Talaromyces emer ...
Rasamsonia emersonii
ACS Omega
2
1660-1665
2017
-
1
1
-
-
-
-
-
-
-
-
-
-
1
-
1
-
-
-
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1
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1
1
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1
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-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
741724
Ozawa
Identification and characteri ...
Rasamsonia emersonii, Thermoascus crustaceus, Thermoascus crustaceus NBRC 9129
Appl. Microbiol. Biotechnol.
101
173-183
2017
-
-
2
-
-
-
-
4
2
-
-
-
-
10
-
2
-
-
-
-
2
-
6
2
4
4
8
-
3
-
4
2
-
-
-
-
-
2
2
-
-
-
-
-
-
4
2
-
-
-
-
-
2
-
-
-
2
-
6
2
4
4
8
-
3
-
4
-
-
-
-
-
-
-
740120
Shiota
An Fe-S cluster in the conserv ...
Burkholderia cepacia
Bioelectrochemistry
112
178-183
2016
-
-
-
-
4
-
-
2
-
-
-
-
-
2
-
-
1
-
-
-
-
-
1
1
-
-
-
-
-
-
-
2
-
-
-
-
-
-
2
-
4
-
-
-
-
2
-
-
-
-
-
-
-
1
-
-
-
-
1
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
740363
Iwasa
-
Thermostable FAD-dependent glu ...
Thermoascus aurantiacus, Thermoascus aurantiacus NBRC 6766, Thermoascus aurantiacus NBRC 9748
Electrochemistry
84
342-348
2016
-
-
1
-
-
-
-
-
-
-
-
-
-
3
-
1
-
-
-
-
-
-
20
-
1
-
1
-
2
-
1
1
-
-
-
-
-
1
1
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
20
-
1
-
1
-
2
-
1
-
-
-
-
-
-
-
739817
Komori
Crystallographic analysis of F ...
Aspergillus terreus
Acta Crystallogr. Sect. F
71
1017-1019
2015
-
-
1
1
-
-
-
-
-
-
-
1
-
2
-
-
1
-
-
-
-
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1
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1
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1
1
1
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1
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1
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-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
740224
Sakai
Stabilization of fungi-derived ...
Aspergillus flavus
Biotechnol. Lett.
37
1091-1099
2015
-
-
1
-
1
-
-
1
-
-
2
-
-
1
-
-
1
-
-
-
-
-
3
1
-
-
1
2
-
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-
1
-
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1
1
-
1
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-
-
1
-
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2
-
-
-
-
1
-
-
-
-
3
1
-
-
1
2
-
-
-
-
-
-
-
-
1
1
740778
Satake
Novel glucose dehydrogenase fr ...
Mucor circinelloides, Mucor circinelloides NISL0103
J. Biosci. Bioeng.
120
498-503
2015
-
-
1
-
-
-
-
1
-
-
-
-
-
4
-
1
1
-
-
1
-
-
14
1
1
1
1
1
1
-
1
1
-
-
-
-
-
1
1
-
-
-
-
-
-
1
-
-
-
-
-
-
1
1
-
1
-
-
14
1
1
1
1
1
1
-
1
-
-
-
-
-
-
-
741412
Yoshida
Structural analysis of fungus- ...
Aspergillus flavus, Aspergillus flavus ATCC 200026
Sci. Rep.
5
13498
2015
-
-
1
1
2
-
-
-
-
-
-
-
-
4
-
-
1
-
-
-
-
-
2
-
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1
-
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1
1
1
2
-
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1
-
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-
2
-
-
-
-
-
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-
-
-
-
-
-
-
-
-
740853
Yang
Efficient expression, purifica ...
Aspergillus terreus, Aspergillus terreus NIH2624
J. Microbiol. Biotechnol.
24
1516-1524
2014
-
-
1
-
-
-
-
1
-
-
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-
7
-
-
1
-
-
-
-
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13
1
1
1
1
-
1
1
1
1
-
-
-
-
-
1
1
-
-
-
-
-
-
1
-
-
-
-
-
-
-
1
-
-
-
-
13
1
1
1
1
-
1
1
1
-
-
-
-
-
-
-
726109
Fapyane
High performance enzyme fuel c ...
Burkholderia cepacia
Phys. Chem. Chem. Phys.
15
9508-9512
2013
-
-
1
-
1
-
-
1
-
-
2
1
-
4
-
-
1
-
-
-
-
-
3
1
-
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1
-
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1
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1
1
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1
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1
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2
1
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1
-
-
-
-
3
1
-
-
-
1
-
-
-
-
-
-
-
-
-
-
740387
Yamashita
Direct electron transfer type ...
Burkholderia cepacia
Enzyme Microb. Technol.
52
123-128
2013
-
-
1
-
38
-
-
-
-
-
-
-
-
3
-
-
1
-
-
-
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2
-
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1
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1
1
-
38
-
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1
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-
-
2
-
-
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-
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-
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-
723928
Zafar
Characterization of different ...
Aspergillus sp., Colletotrichum gloeosporioides, Komagataella pastoris
Anal. Bioanal. Chem.
402
2069-2077
2012
-
3
1
-
3
-
-
-
-
-
4
3
-
6
-
3
-
-
-
1
3
1
8
1
-
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3
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3
1
3
-
3
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4
3
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3
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1
3
1
8
1
-
-
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-
-
-
-
-
-
-
-
-
-
723944
Zafar
Electron-transfer studies with ...
Colletotrichum gloeosporioides
Anal. Chem.
84
334-341
2012
-
-
1
-
1
-
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-
1
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1
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3
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1
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1
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1
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-
1
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1
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724876
Monosik
Amperometric glucose biosensor ...
Aspergillus oryzae
Enzyme Microb. Technol.
50
227-232
2012
-
2
-
-
1
-
-
1
-
-
-
1
-
4
-
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1
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1
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1
1
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1
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2
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1
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1
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1
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1
-
1
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1
1
-
-
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1
1
-
-
-
724639
Mori
Screening of Aspergillus-deriv ...
Aspergillus oryzae, Aspergillus terreus
Biotechnol. Lett.
33
2255-2263
2011
-
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2
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2
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8
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2
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2
2
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2
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8
-
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2
2
-
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725879
Sygmund
Heterologous overexpression of ...
Colletotrichum gloeosporioides
Microb. Cell Fact.
10
106
2011
-
-
1
-
-
-
-
8
-
-
1
1
-
6
-
1
1
-
-
-
1
-
3
1
1
-
1
8
1
-
1
1
-
-
-
-
-
1
1
-
-
-
-
-
-
8
-
-
1
1
-
-
1
1
-
-
1
-
3
1
1
-
1
8
1
-
1
-
-
-
-
-
8
8
725906
Sygmund
Reduction of quinones and phen ...
Colletotrichum gloeosporioides, Colletotrichum gloeosporioides DSM 62728
Microbiology
157
3203-3212
2011
-
-
1
-
-
-
-
-
1
-
2
2
-
7
-
1
1
-
-
-
2
-
11
1
1
-
-
-
2
-
-
1
-
1
-
-
-
1
1
-
-
-
-
-
-
-
1
-
2
2
-
-
1
1
-
-
2
-
11
1
1
-
-
-
2
-
-
1
-
2
2
-
-
-
685757
Yamaoka
Site directed mutagenesis stud ...
Burkholderia cepacia
Biotechnol. Lett.
30
1967-1972
2008
-
1
1
-
38
-
-
4
-
-
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-
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3
-
-
1
-
-
-
9
-
4
-
-
-
-
-
-
-
-
1
-
-
-
-
1
1
1
-
38
-
-
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4
-
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