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Literature summary for 1.1.5.8 extracted from

  • Vangnai, A.S.; Promden, W.; De-Eknamkul, W.; Matsushita, K.; Toyama, H.
    Molecular characterization and heterologous expression of quinate dehydrogenase gene from Gluconobacter oxydans IFO3244 (2010), Biochemistry (Moscow), 75, 452-459.
    View publication on PubMed
Search for more literature for 1.1.5.8

Cloned(Commentary)

Cloned (Comment) Organism
gene qdh, DNA and amino acid sequence determination and analysis, sequence comparisons and phylogenetic analysis, expression in pyrroloquinoline quinone synthesizing Pseudomonas putida strain HK5 Gluconobacter oxydans

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1
-
 quinate pH 8.0, temperature not specified in the publication, recombinant enzyme Gluconobacter oxydans

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
86000
-
 x * 86000, recombinant, unprocessed enzyme, SDS-PAGE Gluconobacter oxydans

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
additional information Gluconobacter oxydans QDH shows higher affinity to quinate, Km 1.0 mM, than to shikimate, Km 9.5 mM ?
-
 ?
additional information Gluconobacter oxydans IFO3244 QDH shows higher affinity to quinate, Km 1.0 mM, than to shikimate, Km 9.5 mM ?
-
 ?
quinate + pyrroloquinoline-quinone Gluconobacter oxydans QDH shows higher affinity to quinate than to shikimate 3-dehydroquinate + reduced pyrroloquinoline-quinone
-
 ?
quinate + pyrroloquinoline-quinone Gluconobacter oxydans IFO3244 QDH shows higher affinity to quinate than to shikimate 3-dehydroquinate + reduced pyrroloquinoline-quinone
-
 ?

Organism

Organism UniProt Comment Textmining
Gluconobacter oxydans B9TTF1 gene qdh
-
Gluconobacter oxydans IFO3244 B9TTF1 gene qdh
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information QDH shows higher affinity to quinate, Km 1.0 mM, than to shikimate, Km 9.5 mM Gluconobacter oxydans ?
-
 ?
additional information QDH shows higher affinity to quinate, Km 1.0 mM, than to shikimate, Km 9.5 mM Gluconobacter oxydans IFO3244 ?
-
 ?
quinate + 2,6-dichlorophenol-indophenol
-
 Gluconobacter oxydans 3-dehydroquinate + reduced 2,6-dichlorophenol-indophenol
-
 ?
quinate + 2,6-dichlorophenol-indophenol
-
 Gluconobacter oxydans IFO3244 3-dehydroquinate + reduced 2,6-dichlorophenol-indophenol
-
 ?
quinate + phenazine methosulfate
-
 Gluconobacter oxydans 3-dehydroquinate + reduced phenazine methosulfate
-
 ?
quinate + phenazine methosulfate
-
 Gluconobacter oxydans IFO3244 3-dehydroquinate + reduced phenazine methosulfate
-
 ?
quinate + pyrroloquinoline-quinone
-
 Gluconobacter oxydans 3-dehydroquinate + reduced pyrroloquinoline-quinone
-
 ?
quinate + pyrroloquinoline-quinone QDH shows higher affinity to quinate than to shikimate Gluconobacter oxydans 3-dehydroquinate + reduced pyrroloquinoline-quinone
-
 ?
quinate + pyrroloquinoline-quinone
-
 Gluconobacter oxydans IFO3244 3-dehydroquinate + reduced pyrroloquinoline-quinone
-
 ?
quinate + pyrroloquinoline-quinone QDH shows higher affinity to quinate than to shikimate Gluconobacter oxydans IFO3244 3-dehydroquinate + reduced pyrroloquinoline-quinone
-
 ?

Subunits

Subunits Comment Organism
? x * 86000, recombinant, unprocessed enzyme, SDS-PAGE Gluconobacter oxydans

Synonyms

Synonyms Comment Organism
PQQ-QDH
-
 Gluconobacter oxydans
QDH
-
 Gluconobacter oxydans

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
 assay at Gluconobacter oxydans

Cofactor

Cofactor Comment Organism Structure
pyrroloquinoline-quinone
-
 Gluconobacter oxydans