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Literature summary for 1.1.5.5 extracted from
- Cloning and functional analysis of adhS gene encoding quinoprotein alcohol dehydrogenase subunit III from Acetobacter pasteurianus SKU1108 (2010), Int. J. Food Microbiol., 138, 39-49.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| ethanol | ethanol does not affect the adhS gene expression but induces PQQ-ADH activity | Acetobacter pasteurianus |  |
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| gene adhS, sequence determination and analysis, encoding quinoprotein alcohol dehydrogenase subunit III | Acetobacter pasteurianus |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| A26V | random mutagenesis, the mutation has no effect on PQQ-ADH activity and ethanol oxidizing ability | Acetobacter pasteurianus |
| G55D | random mutagenesis, the mutation has no effect on PQQ-ADH activity and ethanol oxidizing ability | Acetobacter pasteurianus |
| L18Q | random mutagenesis, the mutation has no effect on PQQ-ADH activity and ethanol oxidizing ability | Acetobacter pasteurianus |
| additional information | random mutagenesis of adhS gene, complete loss of PQQ-ADH activity and ethanol oxidizing ability are observed in the mutants lacking of the 140 and 73 amino acid residues at the C-terminal, whereas the lack of 22 amino acid residues at the C-terminal affected neither the PQQ-ADH activity nor ethanol oxidizing ability | Acetobacter pasteurianus |
| T104K | random mutagenesis, the mutation leads to complpete loss of ethanol oxidizing ability | Acetobacter pasteurianus |
| V107A | random mutagenesis, the mutation has no effect on PQQ-ADH activity and ethanol oxidizing ability | Acetobacter pasteurianus |
| V36I | random mutagenesis, the mutation has no effect on PQQ-ADH activity and ethanol oxidizing ability | Acetobacter pasteurianus |
| V54I | random mutagenesis, the mutation has no effect on PQQ-ADH activity and ethanol oxidizing ability | Acetobacter pasteurianus |
| V70A | random mutagenesis, the mutation has no effect on PQQ-ADH activity and ethanol oxidizing ability | Acetobacter pasteurianus |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| membrane | - |
Acetobacter pasteurianus | 16020 | - |
| additional information | the nucleotide sequence of adhS indicates that the 22 kDa protein is synthesized as a preprotein with NH2-terminal 28 amino acids probably acting as a signal sequence for secretion from cytoplasm to periplasm | Acetobacter pasteurianus | - |
- |
| soluble | - |
Acetobacter pasteurianus | - |
- |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ethanol + ubiquinone | Acetobacter pasteurianus | - |
acetaldehyde + ubiquinol | - |
? | |
| ethanol + ubiquinone | Acetobacter pasteurianus SKU1108 | - |
acetaldehyde + ubiquinol | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Acetobacter pasteurianus | - |
genes adhA, adhB, and adhS, encding subunits I , II, and III, respectively | - |
| Acetobacter pasteurianus SKU1108 | - |
genes adhA, adhB, and adhS, encding subunits I , II, and III, respectively | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ethanol + ubiquinone | - |
Acetobacter pasteurianus | acetaldehyde + ubiquinol | - |
? | |
| ethanol + ubiquinone | - |
Acetobacter pasteurianus SKU1108 | acetaldehyde + ubiquinol | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| PQQ-ADH | - |
Acetobacter pasteurianus |
| quinoprotein alcohol dehydrogenase | - |
Acetobacter pasteurianus |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 25 | - |
assay at | Acetobacter pasteurianus |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 6 | - |
assay at | Acetobacter pasteurianus |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| heme | - |
Acetobacter pasteurianus | |
| pyrroloquinoline quinone | - |
Acetobacter pasteurianus | |
Expression
| Organism | Comment | Expression |
|---|---|---|
| Acetobacter pasteurianus | ethanol does not affect the adhS gene expression but induces PQQ-ADH activity | additional information |
General Information
| General Information | Comment | Organism |
|---|---|---|
| additional information | Thr104 might be involved in molecular coupling with subunit I in order to construct active ADH complex, whereas 22 amino acid residues at C-terminal may be not necessary for PQQ-ADH activity | Acetobacter pasteurianus |
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