Cloned (Comment) | Organism |
---|---|
genes adhA and adhB, encoding subunits I and II, DNA and amino acid sequence determination and analysis,phylogenetic tree | Frateuria aurantia |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
membrane | bound | Frateuria aurantia | 16020 | - |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
45000 | - |
1 * 72000 + 1 * 45000, SDS-PAGE | Frateuria aurantia |
72000 | - |
1 * 72000 + 1 * 45000, SDS-PAGE | Frateuria aurantia |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ethanol + ubiquinone | Frateuria aurantia | - |
acetaldehyde + ubiquinol | - |
? | |
ethanol + ubiquinone | Frateuria aurantia LMG 1558 | - |
acetaldehyde + ubiquinol | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Frateuria aurantia | CCU55317 | genes adhA and adhB encoding subunits I and II | - |
Frateuria aurantia LMG 1558 | CCU55317 | genes adhA and adhB encoding subunits I and II | - |
Purification (Comment) | Organism |
---|---|
native enzyme from membranes by anion exchange and cation exchange chromatography, followed by hydroxyapatite chromatography, to near homogeneity | Frateuria aurantia |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
3 | - |
enzyme in cell membranes, pH 4.5, 20°C | Frateuria aurantia |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ethanol + ubiquinone | - |
Frateuria aurantia | acetaldehyde + ubiquinol | - |
? | |
ethanol + ubiquinone | - |
Frateuria aurantia LMG 1558 | acetaldehyde + ubiquinol | - |
? | |
additional information | substrate specificity, overview | Frateuria aurantia | ? | - |
? | |
additional information | substrate specificity, overview | Frateuria aurantia LMG 1558 | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
heterodimer | 1 * 72000 + 1 * 45000, SDS-PAGE | Frateuria aurantia |
Synonyms | Comment | Organism |
---|---|---|
PQQ-ADH | - |
Frateuria aurantia |
PQQ-dependent alcohol dehydrogenase | - |
Frateuria aurantia |
pyrroloquinoline quinone-dependent alcohol dehydrogenase | - |
Frateuria aurantia |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
20 | - |
- |
Frateuria aurantia |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
10 | 50 | activity range, profile overview. No activity above 50°C, maximum activity at 20°C | Frateuria aurantia |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
4.5 | - |
- |
Frateuria aurantia |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
3 | 6 | strong decrease of activity at pH levels below pH 4 and above pH 5.5, and no activity at pH 2.0 and pH 7.0, activity range, profile overview | Frateuria aurantia |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
pyrroloquinoline quinone | dependent on | Frateuria aurantia |
General Information | Comment | Organism |
---|---|---|
evolution | high similarity between genes encoding subunits I and II of PQQ-ADH | Frateuria aurantia |