BRENDA - Enzyme Database
show all sequences of 1.1.5.5

The membrane-bound quinohemoprotein alcohol dehydrogenase from Gluconacetobacter diazotrophicus PAL5 carries a [2Fe-2S] cluster

Gomez-Manzo, S.; Solano-Peralta, A.; Saucedo-Vazquez, J.P.; Escamilla-Marvan, J.E.; Kroneck, P.M.; Sosa-Torres, M.E.; Biochemistry 49, 2409-2415 (2010)

Data extracted from this reference:

Localization
Localization
Commentary
Organism
GeneOntology No.
Textmining
membrane
-
Gluconacetobacter diazotrophicus
16020
-
Metals/Ions
Metals/Ions
Commentary
Organism
Structure
Ca2+
the enzyme contains one calcium ion which is required for cofactor binding and stabilization of the pyrroloquinoline quinone semiquinone radical
Gluconacetobacter diazotrophicus
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
44000
-
1 * 71000 + 1 * 44000, SDS-PAGE
Gluconacetobacter diazotrophicus
71000
-
1 * 71000 + 1 * 44000, SDS-PAGE
Gluconacetobacter diazotrophicus
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Gluconacetobacter diazotrophicus
-
-
-
Gluconacetobacter diazotrophicus PAL5 (ATCC 49037)
-
-
-
Purification (Commentary)
Commentary
Organism
-
Gluconacetobacter diazotrophicus
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
ethanol + ferricyanide
-
711227
Gluconacetobacter diazotrophicus
acetaldehyde + ferrocyanide
-
-
-
?
ethanol + ferricyanide
-
711227
Gluconacetobacter diazotrophicus PAL5 (ATCC 49037)
acetaldehyde + ferrocyanide
-
-
-
?
ethanol + phenazine methosulfate + 2,6-dichlorophenolindophenol
-
711227
Gluconacetobacter diazotrophicus
?
-
-
-
?
ethanol + phenazine methosulfate + 2,6-dichlorophenolindophenol
-
711227
Gluconacetobacter diazotrophicus PAL5 (ATCC 49037)
?
-
-
-
?
Subunits
Subunits
Commentary
Organism
heterodimer
1 * 71000 + 1 * 44000, SDS-PAGE
Gluconacetobacter diazotrophicus
Cofactor
Cofactor
Commentary
Organism
Structure
cytochrome c
ADH contains 4 c-type cytochromes
Gluconacetobacter diazotrophicus
heme
ADH is a typical quinohemoprotein
Gluconacetobacter diazotrophicus
pyrroloquinoline quinone
ADH is a typical quinohemoprotein with one pyrroloquinoline quinone
Gluconacetobacter diazotrophicus
[2Fe-2S]-center
ADH contains 5.9 Fe2+ and 2.06 acid-labile sulfurs per heterodimer
Gluconacetobacter diazotrophicus
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
cytochrome c
ADH contains 4 c-type cytochromes
Gluconacetobacter diazotrophicus
heme
ADH is a typical quinohemoprotein
Gluconacetobacter diazotrophicus
pyrroloquinoline quinone
ADH is a typical quinohemoprotein with one pyrroloquinoline quinone
Gluconacetobacter diazotrophicus
[2Fe-2S]-center
ADH contains 5.9 Fe2+ and 2.06 acid-labile sulfurs per heterodimer
Gluconacetobacter diazotrophicus
Localization (protein specific)
Localization
Commentary
Organism
GeneOntology No.
Textmining
membrane
-
Gluconacetobacter diazotrophicus
16020
-
Metals/Ions (protein specific)
Metals/Ions
Commentary
Organism
Structure
Ca2+
the enzyme contains one calcium ion which is required for cofactor binding and stabilization of the pyrroloquinoline quinone semiquinone radical
Gluconacetobacter diazotrophicus
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
44000
-
1 * 71000 + 1 * 44000, SDS-PAGE
Gluconacetobacter diazotrophicus
71000
-
1 * 71000 + 1 * 44000, SDS-PAGE
Gluconacetobacter diazotrophicus
Purification (Commentary) (protein specific)
Commentary
Organism
-
Gluconacetobacter diazotrophicus
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
ethanol + ferricyanide
-
711227
Gluconacetobacter diazotrophicus
acetaldehyde + ferrocyanide
-
-
-
?
ethanol + ferricyanide
-
711227
Gluconacetobacter diazotrophicus PAL5 (ATCC 49037)
acetaldehyde + ferrocyanide
-
-
-
?
ethanol + phenazine methosulfate + 2,6-dichlorophenolindophenol
-
711227
Gluconacetobacter diazotrophicus
?
-
-
-
?
ethanol + phenazine methosulfate + 2,6-dichlorophenolindophenol
-
711227
Gluconacetobacter diazotrophicus PAL5 (ATCC 49037)
?
-
-
-
?
Subunits (protein specific)
Subunits
Commentary
Organism
heterodimer
1 * 71000 + 1 * 44000, SDS-PAGE
Gluconacetobacter diazotrophicus
Other publictions for EC 1.1.5.5
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
731507
Aquino Neto
High current density PQQ-depen ...
Gluconobacter sp., Gluconobacter sp. DSM 3504
Biosens. Bioelectron.
72
247-254
2015
-
-
-
-
-
-
-
-
-
-
-
2
-
3
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
741374
Rozeboom
Crystal structure of quinone-d ...
Pseudogluconobacter saccharoketogenes, Pseudogluconobacter saccharoketogenes IFO 14464
Protein Sci.
24
2044-2054
2015
-
-
1
1
-
-
1
-
-
2
-
-
-
7
-
-
1
-
-
-
-
-
24
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
1
-
-
-
1
-
-
-
2
-
-
-
-
-
1
-
-
-
-
24
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
740180
Takeda
Effect of amines as activators ...
Pseudomonas putida, Pseudomonas putida KT 2240
Biosci. Biotechnol. Biochem.
78
1195-1198
2014
16
-
-
-
-
-
-
4
-
-
-
-
-
44
-
-
-
-
-
-
-
-
4
-
-
-
-
4
-
-
-
-
-
-
-
16
-
-
-
-
-
-
-
-
-
4
-
-
-
-
-
-
-
-
-
-
-
-
4
-
-
-
-
4
-
-
-
-
-
-
-
-
4
4
724087
Treek
A unique enzyme of acetic acid ...
Frateuria aurantia, Frateuria aurantia LMG 1558
Appl. Microbiol. Biotechnol.
97
7369-7376
2013
-
-
1
-
-
-
-
-
1
-
2
2
-
6
-
-
1
-
-
-
1
-
4
1
1
1
-
-
1
1
-
1
-
-
-
-
-
1
1
-
-
-
-
-
-
-
1
-
2
2
-
-
-
1
-
-
1
-
4
1
1
1
-
-
1
1
-
-
-
1
1
-
-
-
724837
Aquino Neto
-
Direct electron transfer-based ...
Gluconobacter sp., Gluconobacter sp. 33, Gluconobacter sp. DSM 3504 / ATCC 15163
Electrochim. Acta
87
323-329
2013
-
1
-
-
-
-
-
2
-
-
4
6
-
7
-
-
-
-
-
-
-
-
6
1
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
2
-
-
4
6
-
-
-
-
-
-
-
-
6
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
738312
Elliott
A gene cluster responsible for ...
Leptosphaeria maculans, Leptosphaeria maculans JN3
Fungal Genet. Biol.
53
50-58
2013
-
-
-
-
-
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
1
-
-
-
740121
Takeda
The two-step electrochemical o ...
Pseudomonas putida, Pseudomonas putida KT 2240
Bioelectrochemistry
94
75-78
2013
-
-
1
-
-
-
-
4
-
1
-
-
-
42
-
-
1
-
-
-
-
-
27
-
-
-
-
4
1
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
4
-
1
-
-
-
-
-
1
-
-
-
-
27
-
-
-
-
4
1
-
-
-
-
-
-
-
4
4
724402
Gvozdev
Quinone-dependent alcohol dehy ...
Pseudomonas sp.
Biochemistry
77
843-856
2012
-
-
-
-
-
-
-
-
-
1
-
1
-
1
-
-
-
-
-
-
-
-
1
2
-
-
-
-
-
-
-
1
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
1
-
1
-
-
-
-
-
-
-
-
1
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
726014
Krause
Exploring the function of alco ...
Azoarcus sp.
Mol. Plant Microbe Interact.
24
1325-1332
2011
-
-
1
-
1
-
-
-
-
-
-
1
-
5
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
1
-
-
-
-
-
1
1
-
1
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
2
2
-
-
-
711032
Chavez-Pacheco
The quinohaemoprotein alcohol ...
Komagataeibacter xylinus, Komagataeibacter xylinus IFO 13693
Arch. Microbiol.
192
703-713
2010
-
-
-
-
-
-
2
5
1
-
3
-
-
4
-
-
1
-
-
-
-
1
23
1
-
-
-
5
2
-
-
3
-
1
-
-
-
-
3
-
-
-
-
2
-
5
1
-
3
-
-
-
-
1
-
-
-
1
23
1
-
-
-
5
2
-
-
1
-
-
-
-
5
5
711227
Gomez-Manzo
The membrane-bound quinohemopr ...
Gluconacetobacter diazotrophicus, Gluconacetobacter diazotrophicus PAL5 (ATCC 49037)
Biochemistry
49
2409-2415
2010
-
-
-
-
-
-
-
-
1
1
2
-
-
6
-
-
1
-
-
-
-
-
4
1
-
-
-
-
-
-
-
4
-
-
-
-
-
-
4
-
-
-
-
-
-
-
1
1
2
-
-
-
-
1
-
-
-
-
4
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
724042
Kanchanarach
Characterization of thermotole ...
Acetobacter pasteurianus, Acetobacter pasteurianus IFO3191, Acetobacter pasteurianus IFO3284, Acetobacter pasteurianus MSU10, Acetobacter pasteurianus SKU1108
Appl. Microbiol. Biotechnol.
85
741-751
2010
-
-
-
-
-
-
-
-
-
-
-
5
1
13
-
-
-
-
-
2
-
-
5
-
3
-
2
-
1
-
-
1
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
5
1
-
-
-
-
2
-
-
5
-
3
-
2
-
1
-
-
-
-
-
-
-
-
-
724044
Yakushi
Alcohol dehydrogenase of aceti ...
Acetobacter lovaniensis, Acetobacter lovaniensis IFO3284, Acetobacter pasteurianus, Acetobacter pasteurianus IFO3191, Acetobacter pasteurianus KKP584, Acetobacter pasteurianus MSU10, Acetobacter pasteurianus NCI1452, Acetobacter pasteurianus SKU1108, Acidomonas methanolica, Acidomonas methanolica JCM 6891, Gluconacetobacter diazotrophicus, Gluconacetobacter diazotrophicus PAL5, Gluconacetobacter polyoxogenes, Gluconacetobacter polyoxogenes NBI1028, Gluconobacter oxydans, Gluconobacter oxydans IFO12528, Komagataeibacter europaeus, Komagataeibacter europaeus V3 / LMG 18494, Komagataeibacter intermedius, Komagataeibacter intermedius JK3, Komagataeibacter xylinus
Appl. Microbiol. Biotechnol.
86
1257-1265
2010
-
9
-
-
-
-
-
-
6
-
28
44
-
32
-
-
-
-
-
-
-
-
52
9
-
-
-
-
-
2
-
12
-
-
-
-
9
-
12
-
-
-
-
-
-
-
6
-
28
44
-
-
-
-
-
-
-
-
52
9
-
-
-
-
-
2
-
-
-
19
19
-
-
-
725154
Masud
Cloning and functional analysi ...
Acetobacter pasteurianus, Acetobacter pasteurianus SKU1108
Int. J. Food Microbiol.
138
39-49
2010
1
-
1
-
9
-
-
-
3
-
-
2
-
7
-
-
-
-
-
-
-
-
2
-
1
-
-
-
1
-
-
2
-
-
-
1
-
1
2
-
9
-
-
-
-
-
3
-
-
2
-
-
-
-
-
-
-
-
2
-
1
-
-
-
1
-
-
-
1
1
1
1
-
-
725298
Chattopadhyay
PQQ-dependent alcohol dehydrog ...
Pseudomonas aeruginosa
J. Basic Microbiol.
50
119-124
2010
-
-
1
-
-
-
-
5
1
1
-
1
-
4
-
-
1
-
-
-
16
-
17
-
1
-
-
-
1
-
-
1
-
-
-
-
-
1
1
-
-
-
-
-
-
5
1
1
-
1
-
-
-
1
-
-
16
-
17
-
1
-
-
-
1
-
-
-
-
2
2
-
-
-
692812
Quintero
Quantification of the expressi ...
Acetobacter aceti, Acetobacter pasteurianus
J. Appl. Microbiol.
106
666-674
2009
-
2
-
-
-
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
685685
Matsushita
A tightly bound quinone functi ...
Gluconobacter oxydans, Gluconobacter oxydans IFO 12528
Biosci. Biotechnol. Biochem.
72
2723-2731
2008
-
-
-
-
-
-
3
4
1
-
-
2
-
7
-
-
1
-
-
-
-
-
4
-
1
-
-
-
1
1
-
3
1
-
-
-
-
-
3
-
-
-
-
3
1
4
1
-
-
2
-
-
-
1
-
-
-
-
4
-
1
-
-
-
1
1
-
-
-
-
-
-
-
-
687166
Gomez-Manzo
The PQQ-alcohol dehydrogenase ...
Gluconacetobacter diazotrophicus
Int. J. Food Microbiol.
125
71-78
2008
-
-
-
-
-
-
-
-
1
-
3
-
-
3
-
-
1
-
-
-
1
-
7
1
-
-
1
-
1
1
-
2
-
1
-
-
-
-
2
-
-
-
-
-
-
-
1
-
3
-
-
-
-
1
-
-
1
-
7
1
-
-
1
-
1
1
-
1
-
-
-
-
-
-
686622
Trcek
The highly tolerant acetic aci ...
Komagataeibacter europaeus, Komagataeibacter europaeus V3 / LMG 18494
Extremophiles
11
627-635
2007
1
-
-
-
-
-
-
-
1
-
-
2
-
7
-
-
-
-
-
1
-
-
2
-
-
-
-
-
1
-
-
1
-
-
-
1
-
-
1
-
-
-
-
-
-
-
1
-
-
2
-
-
-
-
-
1
-
-
2
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
684589
Trcek
Correlation between acetic aci ...
Acetobacter pasteurianus, Komagataeibacter europaeus, Komagataeibacter intermedius, Komagataeibacter intermedius JK3
Appl. Microbiol. Biotechnol.
70
366-373
2006
-
-
3
-
-
-
-
-
3
-
7
4
-
6
-
-
3
-
-
1
3
-
8
3
-
-
-
-
3
-
-
3
-
-
-
-
-
3
3
-
-
-
-
-
-
-
3
-
7
4
-
-
-
3
-
1
3
-
8
3
-
-
-
-
3
-
-
-
-
-
-
-
-
-
685650
Shinagawa
A novel type of formaldehyde-o ...
Acetobacter sp., Acetobacter sp. SKU 14
Biosci. Biotechnol. Biochem.
70
850-857
2006
-
-
-
-
-
-
-
-
1
-
3
-
-
3
-
-
1
-
-
1
1
-
5
1
1
1
3
-
1
-
3
2
-
-
-
-
-
-
2
-
-
-
-
-
-
-
1
-
3
-
-
-
-
1
-
1
1
-
5
1
1
1
3
-
1
-
3
-
-
-
-
-
-
-
684665
Toyama
Quinohemoprotein alcohol dehyd ...
Acetobacter aceti, Acetobacter pasteurianus, Acetobacter pasteurianus SKU1108, Acidomonas methanolica, Gluconacetobacter polyoxogenes, Gluconobacter oxydans
Arch. Biochem. Biophys.
428
10-21
2004
-
-
5
-
-
-
-
-
5
-
-
2
-
10
-
-
-
-
-
-
-
-
8
5
-
-
-
-
-
-
-
10
-
-
-
-
-
5
10
-
-
-
-
-
-
-
5
-
-
2
-
-
-
-
-
-
-
-
8
5
-
-
-
-
-
-
-
-
-
-
-
-
-
-
685695
Laurinavicius
Wiring of PQQ-dehydrogenases ...
Gluconobacter sp., Gluconobacter sp. DSM 3504 / ATCC 15163
Biosens. Bioelectron.
20
1217-1222
2004
-
1
-
-
-
-
-
1
-
1
-
-
-
6
-
-
-
-
-
-
1
-
-
1
-
-
-
-
-
-
-
1
-
-
-
-
1
-
1
-
-
-
-
-
-
1
-
1
-
-
-
-
-
-
-
-
1
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
686376
Razumiene
-
4-Ferrocenylphenol as an elect ...
Gluconobacter sp., Gluconobacter sp. DSM 3504 / ATCC 15163
Electrochem. Commun.
2
307-311
2000
-
-
-
-
1
-
-
-
-
-
-
-
-
6
-
-
-
-
-
-
1
-
-
-
-
-
-
-
1
-
-
1
-
-
-
-
-
-
1
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
677303
Chen
Crystallization and preliminar ...
Gluconobacter oxydans
Acta Crystallogr. Sect. D
55
1933-1936
1999
-
-
-
1
-
-
-
-
1
1
-
-
-
4
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
3
-
-
-
-
-
-
3
1
-
-
-
-
-
-
1
1
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
684415
Ramanavicius
Polypyrrole-entrapped quinohem ...
Gluconobacter sp., Gluconobacter sp. DSM 3504 / ATCC 15163
Anal. Chem.
71
3581-3586
1999
-
1
-
-
-
1
-
-
-
1
-
-
-
8
-
-
1
-
-
-
1
-
2
1
1
-
-
-
1
-
-
2
-
-
-
-
1
-
2
-
-
1
-
-
-
-
-
1
-
-
-
-
-
1
-
-
1
-
2
1
1
-
-
-
1
-
-
-
-
-
-
-
-
-
685310
Frebortova
Intramolecular electron transp ...
Acidomonas methanolica, Acidomonas methanolica JCM 6891
Biochim. Biophys. Acta
1363
24-34
1998
-
-
-
-
-
-
-
1
2
-
-
2
-
7
-
-
1
-
-
-
-
-
6
-
1
-
-
-
1
1
-
3
-
-
-
-
-
-
3
-
-
-
-
-
-
1
2
-
-
2
-
-
-
1
-
-
-
-
6
-
1
-
-
-
1
1
-
-
-
-
-
-
-
-
690794
Cozier
The structure of the quinoprot ...
Acetobacter aceti
Biochem. J.
308
375-379
1995
-
-
-
-
-
-
-
-
1
1
-
-
-
3
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
3
-
-
-
-
-
-
3
-
-
-
-
-
-
-
1
1
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-