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Literature summary for 1.1.5.4 extracted from
- Purification and characterization of a malate quinone oxidoreductase from Pseudomonas taetrolens capable of producing valuable lactobionic acid (2020), J. Agric. Food Chem., 68, 13770-13778 .
Application
| Application | Comment | Organism |
|---|---|---|
| industry | the lactose-oxidizing activity of malate:quinone oxidoreductase can be used for producing lactobionic acid (LBA), a valuable organic acid used in the cosmetic, food, and pharmaceutical industries | Pseudomonas taetrolens |
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expression in Escherichia coli | Pseudomonas taetrolens |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| AlCl3 | 1 mM, 30.1% of the activity without metal ion | Pseudomonas taetrolens |  |
| CaCl2 | 1 mM, 72.8% of the activity without metal ion | Pseudomonas taetrolens | |
| CoCl2 | 1 mM, 63.7% of the activity without metal ion | Pseudomonas taetrolens | |
| CuCl2 | 1 mM, 12.4% of the activity without metal ion | Pseudomonas taetrolens | |
| KCl | 1 mM, 97.5% of the activity without metal ion | Pseudomonas taetrolens | |
| MgCl2 | 1 mM, 67.3% of the activity without metal ion | Pseudomonas taetrolens | |
| MnCl2 | 1 mM, 75.2% of the activity without metal ion | Pseudomonas taetrolens | |
| NaCl | 1 mM, 95.3% of the activity without metal ion | Pseudomonas taetrolens | |
| NiCl2 | 1 mM, 65.1% of the activity without metal ion | Pseudomonas taetrolens | |
| ZnCl2 | 1 mM, 29.7% of the activity without metal ion | Pseudomonas taetrolens | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.08 | - |
maltose | pH 5.5, 30°C | Pseudomonas taetrolens | |
| 0.27 | - |
(S)-malate | pH 5.5, 30°C | Pseudomonas taetrolens | |
| 0.59 | - |
cellobiose | pH 5.5, 30°C | Pseudomonas taetrolens | |
| 0.71 | - |
lactose | pH 5.5, 30°C | Pseudomonas taetrolens | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| FeCl2 | 1 mM, 9.4% increase in activity | Pseudomonas taetrolens | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 54000 | - |
gel filtration | Pseudomonas taetrolens |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Pseudomonas taetrolens | A0A0J6JSQ8 | - |
- |
| Pseudomonas taetrolens ATCC 4683 | A0A0J6JSQ8 | - |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Pseudomonas taetrolens |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 9.3 | - |
pH 5.5, 30°C, substrate: lactose | Pseudomonas taetrolens |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| (S)-malate + 2,6-dichlorophenol indophenol | 49.3% of the activity with lactose | Pseudomonas taetrolens | oxaloacetate + reduced 2,6-dichlorophenol indophenol | - |
? | |
| (S)-malate + 2,6-dichlorophenol indophenol | 49.3% of the activity with lactose | Pseudomonas taetrolens ATCC 4683 | oxaloacetate + reduced 2,6-dichlorophenol indophenol | - |
? | |
| cellobiose + 2,6-dichlorophenolindophenol | 64.3% of the activity with lactose | Pseudomonas taetrolens | ? + reduced 2,6-dichlorophenolindophenol | - |
? | |
| cellobiose + 2,6-dichlorophenolindophenol | 64.3% of the activity with lactose | Pseudomonas taetrolens ATCC 4683 | ? + reduced 2,6-dichlorophenolindophenol | - |
? | |
| lactose + 2,6-dichlorophenolindophenol | - |
Pseudomonas taetrolens | lactobionic acid + reduced 2,6-dichlorophenolindophenol | - |
? | |
| lactose + 2,6-dichlorophenolindophenol | - |
Pseudomonas taetrolens ATCC 4683 | lactobionic acid + reduced 2,6-dichlorophenolindophenol | - |
? | |
| maltose + 2,6-dichlorophenolindophenol | - |
Pseudomonas taetrolens | ? + reduced 2,6-dichlorophenolindophenol | - |
? | |
| maltose + 2,6-dichlorophenolindophenol | - |
Pseudomonas taetrolens ATCC 4683 | ? + reduced 2,6-dichlorophenolindophenol | - |
? | |
| additional information | the enzyme only oxidized disaccharides with reducing-end glucosyl residues, such as lactose, but not monosaccharides | Pseudomonas taetrolens | ? | - |
- |
|
| additional information | the enzyme only oxidized disaccharides with reducing-end glucosyl residues, such as lactose, but not monosaccharides | Pseudomonas taetrolens ATCC 4683 | ? | - |
- |
|
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| malate:quinone oxidoreductase | - |
Pseudomonas taetrolens |
| Mqo | - |
Pseudomonas taetrolens |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 30 | - |
- |
Pseudomonas taetrolens |
Temperature Range [°C]
| Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 15 | 50 | 15°C: about 80% of maximal activity, 50°C: about 65% of maximal activity | Pseudomonas taetrolens |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 20 | - |
1 h, no loss of activity | Pseudomonas taetrolens |
| 35 | - |
1 h, 10% loss of activity | Pseudomonas taetrolens |
| 40 | - |
1 h, 30% loss of activity | Pseudomonas taetrolens |
| 45 | - |
1 h, 80% loss of activity | Pseudomonas taetrolens |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 7.22 | - |
maltose | pH 5.5, 30°C | Pseudomonas taetrolens | |
| 7.35 | - |
(S)-malate | pH 5.5, 30°C | Pseudomonas taetrolens | |
| 10.55 | - |
cellobiose | pH 5.5, 30°C | Pseudomonas taetrolens | |
| 14.67 | - |
lactose | pH 5.5, 30°C | Pseudomonas taetrolens | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7 | - |
- |
Pseudomonas taetrolens |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 6 | 12 | pH 6.0: about 60% of maximal activity, pH 12.0: about 50% of maximal activity | Pseudomonas taetrolens |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 1 | 2 | 1 h, about 50% loss of activity | Pseudomonas taetrolens |
| 5 | - |
1 h, about 70% loss of activity | Pseudomonas taetrolens |
| 6 | - |
1 h, about 60% loss of activity | Pseudomonas taetrolens |
| 7 | - |
1 h, no loss of activity | Pseudomonas taetrolens |
| 8 | - |
1 h, about 15% loss of activity | Pseudomonas taetrolens |
| 9 | - |
1 h, about 35% loss of activity | Pseudomonas taetrolens |
| 10 | - |
1 h, about 40% loss of activity | Pseudomonas taetrolens |
kcat/KM [mM/s]
| kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 8.61 | - |
maltose | pH 5.5, 30°C | Pseudomonas taetrolens | |
| 17.86 | - |
cellobiose | pH 5.5, 30°C | Pseudomonas taetrolens | |
| 20.76 | - |
lactose | pH 5.5, 30°C | Pseudomonas taetrolens | |
| 27.66 | - |
(S)-malate | pH 5.5, 30°C | Pseudomonas taetrolens | |
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