Activating Compound | Comment | Organism | Structure |
---|---|---|---|
Phospholipid | in absence of either cardiolipin or vitamin K-3 the enzyme shows about 3% of maximal activity | Mycobacterium sp. | |
vitamin K3 | in absence of either cardiolipin or vitamin K-3 the enzyme shows about 3% of maximal activity | Mycobacterium sp. |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
51000 | 55000 | gel filtration, sucrose density gradient centrifugation | Mycobacterium sp. |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Mycobacterium sp. | - |
- |
- |
Mycobacterium sp. Takeo | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Mycobacterium sp. |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
310 | - |
- |
Mycobacterium sp. |
Storage Stability | Organism |
---|---|
-20°C, 0.3 M potassium phosphate buffer, pH 6.6, about 20% loss of activity after 2 weeks | Mycobacterium sp. |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
(S)-malate + 2,6-dichlorophenol indophenol | - |
Mycobacterium sp. | oxaloacetate + reduced 2,6-dichlorophenol indophenol | - |
? | |
(S)-malate + 2,6-dichlorophenol indophenol | - |
Mycobacterium sp. Takeo | oxaloacetate + reduced 2,6-dichlorophenol indophenol | - |
? |
Synonyms | Comment | Organism |
---|---|---|
FAD-dependent malate dehydrogenase | - |
Mycobacterium sp. |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
FAD | in absence of FAD no reduction of 2,6-dichlorophenol indophenol is observed | Mycobacterium sp. |
Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|
Mycobacterium sp. | isoelectric focusing, pH-range 6-8 | - |
6.9 |