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Literature summary for 1.1.5.4 extracted from
- Malate dehydrogenase (FAD-linked) from Pseudomonas ovalis Chester (1969), Methods Enzymol., 13, 135-140.
No PubMed abstract available
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| Phospholipid | activates | Pseudomonas putida |  |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| Sodium amytal | 1 mM, competitive with respect to phosphatidylethanolamine, noncompetitive with respect to FAD | Pseudomonas putida | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.0024 | - |
ubiquinone-9 | - |
Pseudomonas putida | |
| 0.45 | - |
(S)-malate | - |
Pseudomonas putida | |
| 3 | - |
vitamin K3 | - |
Pseudomonas putida | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| membrane | firmly bound to | Pseudomonas putida | 16020 | - |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Pseudomonas putida | - |
Chester | - |
| Pseudomonas putida Chester | - |
Chester | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Pseudomonas putida |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 9.3 | - |
- |
Pseudomonas putida |
Storage Stability
| Storage Stability | Organism |
|---|---|
| -15°C, 24 h, 70% loss of activity | Pseudomonas putida |
| 0°C, 24 h, 50% loss of activity | Pseudomonas putida |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| (S)-malate + oxidized 2,6-dichlorophenol indophenol | - |
Pseudomonas putida | oxaloacetate + reduced 2,6-dichlorophenol indophenol | - |
? | |
| (S)-malate + oxidized 2,6-dichlorophenol indophenol | - |
Pseudomonas putida Chester | oxaloacetate + reduced 2,6-dichlorophenol indophenol | - |
? | |
| (S)-malate + ubiquinone-0 | - |
Pseudomonas putida | oxaloacetate + ubiquinol-0 | - |
? | |
| (S)-malate + ubiquinone-0 | - |
Pseudomonas putida Chester | oxaloacetate + ubiquinol-0 | - |
? | |
| (S)-malate + ubiquinone-6 | - |
Pseudomonas putida | oxaloacetate + ubiquinol-6 | - |
? | |
| (S)-malate + ubiquinone-6 | - |
Pseudomonas putida Chester | oxaloacetate + ubiquinol-6 | - |
? | |
| (S)-malate + ubiquinone-9 | - |
Pseudomonas putida | oxaloacetate + ubiquinol-9 | - |
? | |
| (S)-malate + ubiquinone-9 | - |
Pseudomonas putida Chester | oxaloacetate + ubiquinol-9 | - |
? | |
| (S)-malate + vitamin K1 | - |
Pseudomonas putida | oxaloacetate + reduced vitamin K1 | - |
? | |
| (S)-malate + vitamin K1 | - |
Pseudomonas putida Chester | oxaloacetate + reduced vitamin K1 | - |
? | |
| (S)-malate + vitamin K3 | - |
Pseudomonas putida | oxaloacetate + reduced vitamin K3 | - |
? | |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 20 | - |
24 h, 85% loss of activity | Pseudomonas putida |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| FAD | Km: 0.0004 mM | Pseudomonas putida | |
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