BRENDA - Enzyme Database
show all sequences of 1.1.5.3

Chemical and functional properties of the native and reconstituted forms of the membrane-bound, aerobic glycerol-3-phosphate dehydrogenase of Escherichia coli

Schryvers, A.; Lohmeier, E.; Weiner, J.H.; J. Biol. Chem. 253, 783-788 (1978)

Data extracted from this reference:

Activating Compound
Activating Compound
Commentary
Organism
Structure
detergent
detergent-depleted enzyme requires exogenous phospholipid or nondenaturing detergent for activity
Escherichia coli
Phospholipid
detergent-depleted enzyme requires exogenous phospholipid or nondenaturing detergent for activity
Escherichia coli
Inhibitors
Inhibitors
Commentary
Organism
Structure
1,10-phenanthroline
-
Escherichia coli
D-2-Phosphoglyceric acid
-
Escherichia coli
D-3-phosphoglyceric acid
-
Escherichia coli
dihydroxyacetone phosphate
-
Escherichia coli
NEM
-
Escherichia coli
PCMB
-
Escherichia coli
phosphoenolpyruvate
-
Escherichia coli
Phosphoglycolic acid
-
Escherichia coli
Localization
Localization
Commentary
Organism
GeneOntology No.
Textmining
membrane
-
Escherichia coli
16020
-
Metals/Ions
Metals/Ions
Commentary
Organism
Structure
Ca2+
-
Escherichia coli
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
58000
-
2 * 58000, SDS-PAGE
Escherichia coli
130000
-
gel filtration
Escherichia coli
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Escherichia coli
-
-
-
Purification (Commentary)
Commentary
Organism
-
Escherichia coli
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
sn-glycerol 3-phosphate + 2,6-dichlorophenolindophenol
-
287818
Escherichia coli
glycerone phosphate + reduced 2,6-dichlorophenylindophenol
-
-
-
?
sn-glycerol 3-phosphate + ferricyanide
-
287818
Escherichia coli
glycerone phosphate + ferrocyanide
-
-
-
?
sn-glycerol 3-phosphate + menadione
-
287818
Escherichia coli
glycerone phosphate + reduced menadione
-
-
-
?
sn-glycerol 3-phosphate + methylene blue
-
287818
Escherichia coli
glycerone phosphate + reduced methylene blue
-
-
-
?
sn-glycerol 3-phosphate + phenazine methosulfate
-
287818
Escherichia coli
glycerone phosphate + reduced phenazine methosulfate
-
-
-
?
Subunits
Subunits
Commentary
Organism
dimer
2 * 58000, SDS-PAGE
Escherichia coli
Temperature Optimum [°C]
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
44
-
-
Escherichia coli
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
7
9.5
-
Escherichia coli
Cofactor
Cofactor
Commentary
Organism
Structure
FAD
contains 0.4 mol of FAD per protein monomer of 58000 Da
Escherichia coli
Activating Compound (protein specific)
Activating Compound
Commentary
Organism
Structure
detergent
detergent-depleted enzyme requires exogenous phospholipid or nondenaturing detergent for activity
Escherichia coli
Phospholipid
detergent-depleted enzyme requires exogenous phospholipid or nondenaturing detergent for activity
Escherichia coli
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
FAD
contains 0.4 mol of FAD per protein monomer of 58000 Da
Escherichia coli
Inhibitors (protein specific)
Inhibitors
Commentary
Organism
Structure
1,10-phenanthroline
-
Escherichia coli
D-2-Phosphoglyceric acid
-
Escherichia coli
D-3-phosphoglyceric acid
-
Escherichia coli
dihydroxyacetone phosphate
-
Escherichia coli
NEM
-
Escherichia coli
PCMB
-
Escherichia coli
phosphoenolpyruvate
-
Escherichia coli
Phosphoglycolic acid
-
Escherichia coli
Localization (protein specific)
Localization
Commentary
Organism
GeneOntology No.
Textmining
membrane
-
Escherichia coli
16020
-
Metals/Ions (protein specific)
Metals/Ions
Commentary
Organism
Structure
Ca2+
-
Escherichia coli
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
58000
-
2 * 58000, SDS-PAGE
Escherichia coli
130000
-
gel filtration
Escherichia coli
Purification (Commentary) (protein specific)
Commentary
Organism
-
Escherichia coli
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
sn-glycerol 3-phosphate + 2,6-dichlorophenolindophenol
-
287818
Escherichia coli
glycerone phosphate + reduced 2,6-dichlorophenylindophenol
-
-
-
?
sn-glycerol 3-phosphate + ferricyanide
-
287818
Escherichia coli
glycerone phosphate + ferrocyanide
-
-
-
?
sn-glycerol 3-phosphate + menadione
-
287818
Escherichia coli
glycerone phosphate + reduced menadione
-
-
-
?
sn-glycerol 3-phosphate + methylene blue
-
287818
Escherichia coli
glycerone phosphate + reduced methylene blue
-
-
-
?
sn-glycerol 3-phosphate + phenazine methosulfate
-
287818
Escherichia coli
glycerone phosphate + reduced phenazine methosulfate
-
-
-
?
Subunits (protein specific)
Subunits
Commentary
Organism
dimer
2 * 58000, SDS-PAGE
Escherichia coli
Temperature Optimum [°C] (protein specific)
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
44
-
-
Escherichia coli
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
7
9.5
-
Escherichia coli
Other publictions for EC 1.1.5.3
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
740482
Lalle
The FAD-dependent glycerol-3-p ...
Giardia intestinalis, Giardia intestinalis WB-C6
Front. Microbiol.
6
544
2015
-
-
1
-
-
-
2
-
1
-
2
2
-
4
-
-
1
-
-
-
-
-
4
1
-
-
-
-
-
-
-
1
-
-
2
-
-
1
1
-
-
-
2
2
-
-
1
-
2
2
-
-
-
1
-
-
-
-
4
1
-
-
-
-
-
-
-
-
-
1
1
-
-
-
739987
Sato
The role of glycerol-3-phospha ...
Mus musculus
Biochem. Biophys. Res. Commun.
444
525-530
2014
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
1
-
-
740537
Rauchova
Inhibition of mitochondrial gl ...
Mesocricetus auratus, Rattus norvegicus
Int. J. Biochem. Cell Biol.
53
409-413
2014
-
-
-
-
-
-
2
-
2
-
-
2
-
4
-
-
-
-
-
2
-
-
2
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
2
-
-
-
-
2
-
-
2
-
-
2
-
-
-
-
-
2
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
741101
Singh
Mitochondrial FAD-linked glyce ...
Homo sapiens
Pharmaceuticals
7
192-206
2014
-
-
-
-
-
-
7
-
1
-
-
1
-
2
-
-
-
-
-
1
-
-
1
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
1
-
-
-
-
7
-
-
1
-
-
1
-
-
-
-
-
1
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
741302
Orr
Novel inhibitors of mitochondr ...
Mus musculus, Mus musculus C57BL/6, Rattus norvegicus, Rattus norvegicus Wistar
PLoS ONE
9
e89938
2014
-
-
-
-
-
-
42
2
5
-
-
4
-
6
-
2
-
-
-
4
-
-
16
-
2
-
-
-
-
-
-
2
4
-
8
-
-
-
2
-
-
-
8
42
4
2
5
-
-
4
-
-
2
-
-
4
-
-
16
-
2
-
-
-
-
-
-
-
-
3
3
-
-
-
740095
Mracek
The function and the role of t ...
Rattus norvegicus
Biochim. Biophys. Acta
1827
401-410
2013
-
-
-
-
-
-
5
-
1
4
1
2
-
1
-
-
-
-
-
1
-
-
2
1
-
-
-
-
-
-
-
1
-
-
-
-
-
-
1
-
-
-
-
5
-
-
1
4
1
2
-
-
-
-
-
1
-
-
2
1
-
-
-
-
-
-
-
-
-
1
1
-
-
-
740404
Skodova
Characterization of two mitoch ...
Trypanosoma brucei, Trypanosoma brucei 29-13
Eukaryot. Cell
12
1664-1673
2013
-
-
-
-
-
-
-
-
1
-
-
2
-
6
-
-
-
-
-
-
-
-
4
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
1
-
-
-
-
-
-
-
1
-
-
2
-
-
-
-
-
-
-
-
4
-
-
-
-
-
-
-
-
-
-
1
1
-
-
-
723350
Rauchova
Idebenone-induced recovery of ...
Rattus norvegicus, Rattus norvegicus Wistar
Physiol. Res.
61
259-265
2012
1
-
-
-
-
-
1
-
1
-
-
2
-
166
-
-
-
-
-
2
-
-
2
-
1
-
-
-
1
-
-
1
-
-
-
1
-
-
1
-
-
-
-
1
-
-
1
-
-
2
-
-
-
-
-
2
-
-
2
-
1
-
-
-
1
-
-
-
-
-
-
-
-
-
713077
Kota
Role of glycerol-3-phosphate d ...
Mus musculus
Mol. Reprod. Dev.
77
773-783
2010
-
-
-
-
-
-
-
-
1
-
1
-
-
3
-
1
-
-
-
2
-
-
1
-
-
-
-
-
-
-
-
1
-
1
-
-
-
-
1
-
-
-
-
-
-
-
1
-
1
-
-
-
1
-
-
2
-
-
1
-
-
-
-
-
-
-
-
1
-
2
2
-
-
-
696426
Denton
Regulation of mitochondrial de ...
Rattus norvegicus
Biochim. Biophys. Acta
1787
1309-1316
2009
-
-
-
-
-
-
-
-
-
1
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
698171
Daoud
Haploinsufficiency of the GPD2 ...
Homo sapiens
Hum. Genet.
124
649-658
2009
-
-
-
-
-
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
685288
Colussi
Structure of alpha-glycerophos ...
Streptococcus sp.
Biochemistry
47
965-977
2008
-
-
1
1
-
-
-
-
-
-
-
-
-
1
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
1
1
1
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
689630
Quettier
SUGAR-DEPENDENT6 encodes a mit ...
Arabidopsis thaliana
Plant Physiol.
148
519-528
2008
-
-
-
-
-
-
-
-
1
-
-
1
-
2
-
-
-
-
-
1
-
-
1
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
1
-
-
-
-
-
-
-
1
-
-
1
-
-
-
-
-
1
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
685325
Vrbacky
Respiratory chain components i ...
Mesocricetus auratus
Biochim. Biophys. Acta
1767
989-997
2007
-
-
-
-
-
-
-
-
1
-
-
1
-
2
-
-
-
-
-
1
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
1
-
-
-
-
-
1
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
687435
Yang
Isolation of a FAD-GPDH gene e ...
Dunaliella salina
J. Basic Microbiol.
47
266-274
2007
-
-
1
-
-
-
-
-
4
-
-
1
-
4
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
1
-
-
-
-
-
1
1
-
-
-
-
-
-
-
4
-
-
1
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
655567
Shen
Identification of a mitochondr ...
Arabidopsis thaliana
FEBS Lett.
536
92-96
2003
-
-
1
-
-
-
-
-
2
-
1
1
-
5
-
-
-
-
-
-
-
-
1
1
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
2
-
1
1
-
-
-
-
-
-
-
-
1
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
695272
Larsson
The importance of the glycerol ...
Saccharomyces cerevisiae
Yeast
14
347-357
1998
-
-
-
-
-
-
-
-
1
1
-
-
-
1
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
1
-
-
-
-
-
-
-
1
1
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
690607
Rauchova
Steady-state kinetics of reduc ...
Mesocricetus auratus, Periplaneta americana
Arch. Biochem. Biophys.
344
235-241
1997
-
-
-
-
-
-
-
-
2
-
-
2
-
3
-
-
-
-
-
2
-
-
10
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
2
-
-
-
-
-
-
-
2
-
-
2
-
-
-
-
-
2
-
-
10
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
287831
MacDonald
Calcium activation of mitochon ...
Rattus norvegicus
Arch. Biochem. Biophys.
326
79-84
1996
-
-
1
-
-
-
-
3
1
1
-
1
-
1
-
-
-
-
-
2
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
3
1
1
-
1
-
-
-
-
-
2
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
690606
Koza
Sequence and tissue-dependent ...
Mus musculus
Arch. Biochem. Biophys.
336
97-104
1996
-
-
-
-
-
-
-
-
2
1
-
-
-
6
-
-
-
-
-
5
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
1
-
-
-
-
-
-
-
2
1
-
-
-
-
-
-
-
5
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
287829
Beleznai
Incorporation of mitochondrial ...
Rattus norvegicus
Biochim. Biophys. Acta
1018
72-76
1990
1
-
-
-
-
-
-
1
1
1
-
-
-
1
-
-
1
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
1
1
1
-
-
-
-
-
1
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
287828
Beleznai
Role of cardiolipin in the fun ...
Rattus norvegicus
Biochem. Biophys. Res. Commun.
159
132-139
1989
1
-
-
-
-
-
1
1
1
-
-
-
-
1
-
-
-
-
-
1
-
-
3
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
1
-
1
1
-
-
-
-
-
-
-
-
1
-
-
3
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
287821
Beleznai
Ca2+ and Mg2+ as modulators of ...
Rattus norvegicus
Eur. J. Biochem.
170
631-636
1988
-
-
-
-
-
-
-
-
1
2
-
-
-
1
-
-
-
-
-
1
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
2
-
-
-
-
-
-
-
1
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
287805
Halsey
Flavin-linked mitochondrial al ...
Cyberlindnera jadinii, Saccharomyces cerevisiae
Biochim. Biophys. Acta
682
387-394
1982
2
-
-
-
-
-
14
1
1
-
1
-
-
2
-
-
1
-
-
-
-
-
3
-
-
-
-
-
1
-
-
1
-
-
-
2
-
-
1
-
-
-
-
14
-
1
1
-
1
-
-
-
-
1
-
-
-
-
3
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
287807
Unemoto
Partial purification and prope ...
Vibrio alginolyticus
J. Biochem.
90
619-628
1981
1
-
-
-
-
-
8
4
1
2
1
1
-
1
-
-
1
1
-
-
1
-
5
-
-
-
-
-
1
-
-
1
-
-
-
1
-
-
1
-
-
-
-
8
-
4
1
2
1
1
-
-
-
1
-
-
1
-
5
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
287817
Schryvers
The anaerobic sn-glycerol-3-ph ...
Escherichia coli
J. Biol. Chem.
256
9959-9965
1981
2
-
-
-
-
-
4
2
1
1
3
-
-
2
-
-
1
-
-
-
-
2
2
1
-
-
-
1
-
-
-
1
-
-
-
2
-
-
1
-
-
-
-
4
-
2
1
1
3
-
-
-
-
1
-
-
-
2
2
1
-
-
-
1
-
-
-
-
-
-
-
-
-
-
287806
Cottingham
Purification and properties of ...
Sus scrofa
Biochem. J.
192
9-18
1980
-
-
-
-
-
-
1
2
1
1
1
-
-
1
-
1
1
-
-
1
1
-
4
1
-
-
-
-
-
-
1
1
-
-
-
-
-
-
1
-
-
-
-
1
-
2
1
1
1
-
-
-
1
1
-
1
1
-
4
1
-
-
-
-
-
-
1
-
-
-
-
-
-
-
287820
Robinson
The effect of amphipaths on th ...
Escherichia coli
Can. J. Biochem.
58
1172-1178
1980
-
-
-
-
-
-
2
2
-
-
-
-
-
4
-
-
1
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
2
-
2
-
-
-
-
-
-
-
1
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
287802
Dawson
-
L-3-Glycerophosphate dehydroge ...
Sus scrofa
Methods Enzymol.
41B
254-259
1978
-
-
-
-
-
1
6
8
1
1
-
-
-
1
-
-
1
-
-
1
1
-
8
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
1
-
-
1
-
6
-
8
1
1
-
-
-
-
-
1
-
1
1
-
8
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
287809
Cottingham
Purification and properties of ...
Sus scrofa
Biochem. Soc. Trans.
6
1307-1310
1978
-
-
-
-
-
-
3
-
1
1
-
-
-
1
-
-
1
-
-
1
-
-
4
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
2
-
-
-
-
3
-
-
1
1
-
-
-
-
-
1
-
1
-
-
4
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
287818
Schryvers
Chemical and functional proper ...
Escherichia coli
J. Biol. Chem.
253
783-788
1978
2
-
-
-
-
-
8
-
1
1
2
-
-
3
-
-
1
-
-
-
-
-
5
1
1
-
-
-
1
-
-
1
-
-
-
2
-
-
1
-
-
-
-
8
-
-
1
1
2
-
-
-
-
1
-
-
-
-
5
1
1
-
-
-
1
-
-
-
-
-
-
-
-
-
287813
Weiner
Purification of the membrane-b ...
Escherichia coli
Biochem. Biophys. Res. Commun.
47
1360-1365
1972
-
-
-
-
-
-
1
1
1
-
2
-
-
1
-
-
1
-
-
-
1
2
1
1
-
-
-
-
-
-
-
1
-
-
-
-
-
-
1
-
-
-
-
1
-
1
1
-
2
-
-
-
-
1
-
-
1
2
1
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
287812
Dawson
Preparation and some propertie ...
Sus scrofa
Biochem. J.
111
27-34
1969
-
-
-
-
-
1
1
-
1
1
-
-
-
1
-
-
1
-
-
1
1
-
1
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
1
-
-
1
-
1
-
-
1
1
-
-
-
-
-
1
-
1
1
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-