Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 1.1.5.14 extracted from

  • Tominaga, M.; Nomura, S.; Taniguchi, I.
    D-fructose detection based on the direct heterogeneous electron transfer reaction of fructose dehydrogenase adsorbed onto multi-walled carbon nanotubes synthesized on platinum electrode (2009), Biosens. Bioelectron., 24, 1184-1188.
    View publication on PubMed
Search for more literature for 1.1.5.14

Application

Application Comment Organism
biotechnology multi-walled carbon nanotubes synthesized on platinum plate (MWCNTs/Pt) electrode are immediately immersed into solutions of FDH to immobilize the enzyme onto electrode surfaces. Thereafter, a well-defined catalytic oxidation current based on FDH is observed from ca. -0.15V, which is close to the redox potential of heme c as a prosthetic group of FDH. From an analysis of a plot of the catalytic current versus substrate, the calibration range for the fructose concentration is up to ca. 40 mmol/dm3, and the apparent Michaelis-Menten constant is evaluated to be 11 mmol/l. The obtained results are useful in applications to prepare the third-generation biosensors and other future bioelectrochemical devices Gluconobacter sp.

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
11
-
 D-fructose Km is determined using FDH adsorbed onto multi-walled carbon nanotubes synthesized on platinum electrode Gluconobacter sp.

Organism

Organism UniProt Comment Textmining
Gluconobacter sp.
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
D-fructose + acceptor
-
 Gluconobacter sp. 5-dehydro-D-fructose + reduced acceptor
-
 ?

Synonyms

Synonyms Comment Organism
FDH
-
 Gluconobacter sp.
fructose dehydrogenase
-
 Gluconobacter sp.

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
5
-
 assay at Gluconobacter sp.

Cofactor

Cofactor Comment Organism Structure
heme c
-
 Gluconobacter sp.