Cloned (Comment) | Organism |
---|---|
heterologously expressed in Escherichia coli C43(DE3) as a His-tagged protein | Pseudomonas putida |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
antimycin A | inhibits electron transfer from quinone to cytochrome c | Pseudomonas putida | |
Ba2+ | 5 mM, partial inhibition of activity | Pseudomonas putida | |
Ca2+ | 5 mM, partial inhibition of activity | Pseudomonas putida | |
Cd2+ | 5 mM, complete inhibition of activity | Pseudomonas putida | |
Co2+ | 5 mM, complete inhibition of activity | Pseudomonas putida | |
Cu2+ | 5 mM, complete inhibition of activity | Pseudomonas putida | |
EDTA | 5 mM, partial inhibition of activity | Pseudomonas putida | |
Fe2+ | 5 mM, complete inhibition of activity | Pseudomonas putida | |
Mg2+ | 5 mM, partial inhibition of activity | Pseudomonas putida | |
Mn2+ | 5 mM, complete inhibition of activity | Pseudomonas putida | |
NaN3 | inhibits electron transfer from cytochrome c to molecular oxygen | Pseudomonas putida | |
Ni2+ | 5 mM, partial inhibition of activity | Pseudomonas putida | |
Zn2+ | 5 mM, complete inhibition of activity | Pseudomonas putida |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0041 | - |
Ubiquinone-10 | pH 7.4, 30°C | Pseudomonas putida | |
0.048 | - |
3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide | pH 7.4, 30°C | Pseudomonas putida | |
0.057 | - |
D-lactate | pH 7.4, 30°C | Pseudomonas putida | |
0.1 | - |
D-2-hydroxybutyrate | pH 7.4, 30°C | Pseudomonas putida | |
1.04 | - |
L-lactate | pH 7.4, 30°C | Pseudomonas putida | |
1.38 | - |
D-glycerate | pH 7.4, 30°C | Pseudomonas putida |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
membrane | membrane-associated | Pseudomonas putida | 16020 | - |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
K+ | 5 mM, slightly positive effect | Pseudomonas putida |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
118000 | - |
gel filtration | Pseudomonas putida |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pseudomonas putida | Q88DT2 | - |
- |
Purification (Comment) | Organism |
---|---|
- |
Pseudomonas putida |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
D-2-hydroxybutyrate + ubiquinone-10 | - |
Pseudomonas putida | 2-oxobutyrate + ubiquinol-10 | - |
? | |
D-glycerate + ubiquinone-10 | - |
Pseudomonas putida | ? + ubiquinol-10 | - |
? | |
D-lactate + 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide | - |
Pseudomonas putida | pyruvate + reduced 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide | - |
? | |
D-lactate + ubiquinone-10 | - |
Pseudomonas putida | pyruvate + ubiquinol-10 | - |
? | |
L-lactate + 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide | - |
Pseudomonas putida | pyruvate + reduced 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide | - |
? | |
L-lactate + ubiquinone-10 | - |
Pseudomonas putida | pyruvate + ubiquinol-10 | - |
? | |
additional information | the enzyme exhibits narrow substrate specificity. No activity with D-2-hydroxybutyrate, L-2-hydroxybutyrate, D-mandelate, L-mandelate, D-3-phenyllactate, D-glycerate, L-glycerate, DL-2-hydroxyisocaproate, and DL-2-hydroxyoctanoate, with 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide as an electron acceptor | Pseudomonas putida | ? | - |
- |
Subunits | Comment | Organism |
---|---|---|
monomer | 1 * 100000, SDS-PAGE | Pseudomonas putida |
Synonyms | Comment | Organism |
---|---|---|
Fe-S D-iLDH | - |
Pseudomonas putida |
lldE | - |
Pseudomonas putida |
NAD-independent D-lactate dehydrogenase | - |
Pseudomonas putida |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
2.4 | - |
Ubiquinone-10 | pH 7.4, 30°C | Pseudomonas putida | |
8 | - |
D-glycerate | pH 7.4, 30°C | Pseudomonas putida | |
9.5 | - |
L-lactate | pH 7.4, 30°C | Pseudomonas putida | |
66 | - |
D-2-hydroxybutyrate | pH 7.4, 30°C | Pseudomonas putida | |
120 | - |
D-lactate | pH 7.4, 30°C | Pseudomonas putida | |
128 | - |
3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide | pH 7.4, 30°C | Pseudomonas putida |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
FAD | the enzyme contains a flavin adenine dinucleotide (FAD)-containing dehydrogenase domain. Compared to the intact enzyme, the FAD-containing dehydrogenase domain shows increased catalytic efficiency with cytochrome c as the electron acceptor, but it completely loses the ability to use coenzyme Q10. The FAD containing dehydrogenase domain is no longer associated with the cell membrane, and it can not support the utilization of D-lactate as a carbon source | Pseudomonas putida | |
Fe-S center | the enzyme contains an Fe-S oxidoreductase domain. The Fe-S oxidoreductase domain functions as an electron transfer component to facilitate the utilization of quinone as an electron acceptor by the enzyme (Fe-S D-iLDH), and it helps the enzyme associate with the cell membrane | Pseudomonas putida |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.59 | - |
Ubiquinone-10 | pH 7.4, 30°C | Pseudomonas putida | |
6 | - |
D-glycerate | pH 7.4, 30°C | Pseudomonas putida | |
9 | - |
L-lactate | pH 7.4, 30°C | Pseudomonas putida | |
660 | - |
D-2-hydroxybutyrate | pH 7.4, 30°C | Pseudomonas putida | |
2100 | - |
D-lactate | pH 7.4, 30°C | Pseudomonas putida | |
2700 | - |
3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide | pH 7.4, 30°C | Pseudomonas putida |