BRENDA - Enzyme Database
show all sequences of 1.1.5.10

Assay, purification and properties of mammalian D-2-hydroxy acid dehydrogenase

Cammack, R.; Biochem. J. 115, 55-64 (1969)

Data extracted from this reference:

Inhibitors
Inhibitors
Commentary
Organism
Structure
oxalate
-
Oryctolagus cuniculus
oxaloacetate
-
Oryctolagus cuniculus
Localization
Localization
Commentary
Organism
GeneOntology No.
Textmining
mitochondrion
-
Oryctolagus cuniculus
5739
-
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
95000
-
combination of gel filtration data and the sedimentation coefficient
Oryctolagus cuniculus
102000
-
gel filtration
Oryctolagus cuniculus
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Oryctolagus cuniculus
-
-
-
Purification (Commentary)
Commentary
Organism
-
Oryctolagus cuniculus
Source Tissue
Source Tissue
Commentary
Organism
Textmining
kidney
cortex
Oryctolagus cuniculus
-
Storage Stability
Storage Stability
Organism
4°C, 50 mM Tris-chloride buffer, pH 8-0, in the dark, stable for several weeks
Oryctolagus cuniculus
4°C, as a precipitate in 32% (w/v) ammonium sulfate solution in 50 mM Tris-chloride buffer, pH 8.0, stable for several months
Oryctolagus cuniculus
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
D-lactate + oxidized 2,6-dichloroindophenol
-
724205
Oryctolagus cuniculus
pyruvate + reduced 2,6-dichloroindophenol
-
-
-
?
D-lactate + ubiquinone
the rate with 0.06 mM ubiquinone is 72% of the rate with 2,6-dichloroindophenol
724205
Oryctolagus cuniculus
pyruvate + ubiquinol
-
-
-
?
Temperature Optimum [°C]
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
30
-
assay at
Oryctolagus cuniculus
Temperature Stability [°C]
Temperature Stability Minimum [°C]
Temperature Stability Maximum [°C]
Commentary
Organism
20
-
pH 5.5-10, 30 min, stable
Oryctolagus cuniculus
62
-
pH 7.3, activity decreases by 79% in 10 min, in the presence of 1 mM oxalate the decrease is 5%
Oryctolagus cuniculus
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
8.6
-
assay at
Oryctolagus cuniculus
pH Stability
pH Stability
pH Stability Maximum
Commentary
Organism
4.8
-
20°C, 30 min, stable above pH 10.5 or below pH 4.8
Oryctolagus cuniculus
5.5
10
20°C, 30 min, stable
Oryctolagus cuniculus
7.3
-
unstable above 55°C
Oryctolagus cuniculus
10.5
-
20°C, 30 min, stable above pH 10.5 or below pH 4.8
Oryctolagus cuniculus
Cofactor
Cofactor
Commentary
Organism
Structure
FAD
the purified enzyme has a spectrum typical of a flavoprotein. The change induced in the spectrum on addition of D-malate or D-lactate suggests the formation of a flavin semiquinone. Flavin can be removed by treatment with acid ammonium sulfate, and activity can be restored to the inactive apoenzyme by addition of FAD, but not of FMN or riboflavin
Oryctolagus cuniculus
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
FAD
the purified enzyme has a spectrum typical of a flavoprotein. The change induced in the spectrum on addition of D-malate or D-lactate suggests the formation of a flavin semiquinone. Flavin can be removed by treatment with acid ammonium sulfate, and activity can be restored to the inactive apoenzyme by addition of FAD, but not of FMN or riboflavin
Oryctolagus cuniculus
Inhibitors (protein specific)
Inhibitors
Commentary
Organism
Structure
oxalate
-
Oryctolagus cuniculus
oxaloacetate
-
Oryctolagus cuniculus
Localization (protein specific)
Localization
Commentary
Organism
GeneOntology No.
Textmining
mitochondrion
-
Oryctolagus cuniculus
5739
-
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
95000
-
combination of gel filtration data and the sedimentation coefficient
Oryctolagus cuniculus
102000
-
gel filtration
Oryctolagus cuniculus
Purification (Commentary) (protein specific)
Commentary
Organism
-
Oryctolagus cuniculus
Source Tissue (protein specific)
Source Tissue
Commentary
Organism
Textmining
kidney
cortex
Oryctolagus cuniculus
-
Storage Stability (protein specific)
Storage Stability
Organism
4°C, 50 mM Tris-chloride buffer, pH 8-0, in the dark, stable for several weeks
Oryctolagus cuniculus
4°C, as a precipitate in 32% (w/v) ammonium sulfate solution in 50 mM Tris-chloride buffer, pH 8.0, stable for several months
Oryctolagus cuniculus
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
D-lactate + oxidized 2,6-dichloroindophenol
-
724205
Oryctolagus cuniculus
pyruvate + reduced 2,6-dichloroindophenol
-
-
-
?
D-lactate + ubiquinone
the rate with 0.06 mM ubiquinone is 72% of the rate with 2,6-dichloroindophenol
724205
Oryctolagus cuniculus
pyruvate + ubiquinol
-
-
-
?
Temperature Optimum [°C] (protein specific)
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
30
-
assay at
Oryctolagus cuniculus
Temperature Stability [°C] (protein specific)
Temperature Stability Minimum [°C]
Temperature Stability Maximum [°C]
Commentary
Organism
20
-
pH 5.5-10, 30 min, stable
Oryctolagus cuniculus
62
-
pH 7.3, activity decreases by 79% in 10 min, in the presence of 1 mM oxalate the decrease is 5%
Oryctolagus cuniculus
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
8.6
-
assay at
Oryctolagus cuniculus
pH Stability (protein specific)
pH Stability
pH Stability Maximum
Commentary
Organism
4.8
-
20°C, 30 min, stable above pH 10.5 or below pH 4.8
Oryctolagus cuniculus
5.5
10
20°C, 30 min, stable
Oryctolagus cuniculus
7.3
-
unstable above 55°C
Oryctolagus cuniculus
10.5
-
20°C, 30 min, stable above pH 10.5 or below pH 4.8
Oryctolagus cuniculus
Other publictions for EC 1.1.5.10
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
727253
Kato
Quinone-dependent D-lactate de ...
Corynebacterium glutamicum, Corynebacterium glutamicum DSM 20300
BMC Microbiol.
10
321
2010
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1
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2
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2
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1
2
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1
1
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726894
Cammack
Mammalian D-2-hydroxy acid deh ...
Oryctolagus cuniculus
Biochem. J.
118
405-408
1970
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1
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1
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1
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1
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1
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1
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1
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1
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1
-
-
-
-
-
-
-
-
-
-
-
-
-
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-
724205
Cammack
Assay, purification and proper ...
Oryctolagus cuniculus
Biochem. J.
115
55-64
1969
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2
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1
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2
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1
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1
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1
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2
2
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1
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2
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1
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4
1
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1
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2
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1
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2
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1
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1
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2
2
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1
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2
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1
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4
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