Literature summary for 1.1.3.9 extracted from

Faria, C.B.; de Castro, F.F.; Martim, D.B.; Abe, C.A.L.; Prates, K.V.; de Oliveira, M.A.S.; Barbosa-Tessmann, I.P.
Production of galactose oxidase inside the Fusarium fujikuroi species complex and recombinant expression and characterization of the galactose oxidase GaoA protein from Fusarium subglutinans (2019), Mol. Biotechnol., 61, 633-649 .

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Activating Compound

Activating Compound	Comment	Organism	Structure
glycerol	25%, 774% of initial activity	Fusarium subglutinans
Tween 80	2.5%, 316% of initial activity	Fusarium subglutinans

Cloned(Commentary)

Cloned (Comment)	Organism
expression in Escherichia coli	Fusarium subglutinans

Inhibitors

Inhibitors	Comment	Organism	Structure
CTAB	5 mM, 77% of initial activity	Fusarium subglutinans
EDTA	5 mM, 81% of initial activity	Fusarium subglutinans
KCl	5 mM, 90% of initial activity	Fusarium subglutinans
NaCl	5 mM, 90% of initial activity	Fusarium subglutinans
SDS	5 mM, 4% of initial activity	Fusarium subglutinans

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
132.6	-	D-galactose	pH 7, 30°C	Fusarium subglutinans

Localization

Localization	Comment	Organism	GeneOntology No.	Textmining
extracellular	protein is secreted, and sequence presents N-terminal secretory and maturation sequences	Fusarium subglutinans	-	-

Metals/Ions

Metals/Ions	Comment	Organism	Structure
Mg2+	5 mM, 107% of initial activity	Fusarium subglutinans

Organic Solvent Stability

Organic Solvent	Comment	Organism
Glycerol	25%, 774% of initial activity	Fusarium subglutinans

Organism

Organism	UniProt	Comment	Textmining
Fusarium subglutinans	A0A0U1YLU5	-	-

Posttranslational Modification

Posttranslational Modification	Comment	Organism
no glycoprotein	sequence contains no putative N-glycosylation sites	Fusarium subglutinans

Purification (Commentary)

Purification (Comment)	Organism
recombinant protein	Fusarium subglutinans

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
alpha-D-melibiose + O2	-	Fusarium subglutinans	? + H2O2	-	?
D-galactose + O2	-	Fusarium subglutinans	D-galacto-hexodialdose + H2O2	-	?
D-raffinose + O2	-	Fusarium subglutinans	? + H2O2	-	?
methyl alpha-D-galactopyranoside + O2	-	Fusarium subglutinans	methyl alpha-D-galacto-hexodialdo-1,5-pyranoside + H2O2	-	?
additional information	no substrate: D-glucose	Fusarium subglutinans	?	-	-

Subunits

Subunits	Comment	Organism
?	x * 77000 and x * 74000, SDS-PAGE, x * 72500, calculated from sequence	Fusarium subglutinans

Synonyms

Synonyms	Comment	Organism
GAOA	-	Fusarium subglutinans

Temperature Optimum [°C]

Temperature Optimum [°C]	Temperature Optimum Maximum [°C]	Comment	Organism
30	-	-	Fusarium subglutinans

Turnover Number [1/s]

Turnover Number Minimum [1/s]	Turnover Number Maximum [1/s]	Substrate	Comment	Organism	Structure
12243	-	D-galactose	pH 7, 30°C	Fusarium subglutinans

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
7	-	-	Fusarium subglutinans

pI Value

Organism	Comment	pI Value Maximum	pI Value
Fusarium subglutinans	calculated from sequence, mature protein	-	7.6

kcat/KM [mM/s]

kcat/KM Value [1/mMs^-1]	kcat/KM Value Maximum [1/mMs^-1]	Substrate	Comment	Organism	Structure
92	-	D-galactose	pH 7, 30°C	Fusarium subglutinans

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Release 2023.1 (January 2023)