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Literature summary for 1.1.3.7 extracted from

  • Vina-Gonzalez, J.; Martinez, A.T.; Guallar, V.; Alcalde, M.
    Sequential oxidation of 5-hydroxymethylfurfural to furan-2,5-dicarboxylic acid by an evolved aryl-alcohol oxidase (2020), Biochim. Biophys. Acta, 1868, 140293 .
    View publication on PubMed
Search for more literature for 1.1.3.7

Protein Variants

Protein Variants Comment Organism
H91N/L170M mutant with increased activity on 5-hydroxymethylfurfural and its oxidation products Pleurotus eryngii
H91N/L170M/F501W mutant with increased activity on 5-hydroxymethylfurfural and its oxidation products Pleurotus eryngii
H91N/L170M/I500M/F501W mutant with increased activity on 5-hydroxymethylfurfural and its oxidation products Pleurotus eryngii

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.6
-
 5-hydroxymethylfurfural wild-type, pH 6, 25°C Pleurotus eryngii
12.4
-
 5-hydroxymethylfurfural mutant H91N/L170M/F501W, pH 6, 25°C Pleurotus eryngii
13
-
 5-hydroxymethylfurfural mutant H91N/L170M, pH 6, 25°C Pleurotus eryngii

Organism

Organism UniProt Comment Textmining
Pleurotus eryngii O94219 cf. EC 1.1.3.47
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2,5-diformylfuran + O2
-
 Pleurotus eryngii formylfurancarboxylic acid + ?
-
 ?
4-anisaldehyde + O2
-
 Pleurotus eryngii 4-anisic acid + H2O2
-
 ?
4-methoxybenzyl alcohol + O2
-
 Pleurotus eryngii 4-methoxybenzyl aldehyde + H2O2
-
 ?
5-hydroxymethylfurfural + O2
-
 Pleurotus eryngii 2,5-diformylfuran + H2O2
-
 ?
formylfurancarboxylic acid + O2
-
 Pleurotus eryngii 2,5-furandicarboxylic acid + H2O2
-
 ?
additional information for complete oxidation of 5-hydroxymethylfurfural, the rate-limiting step lies in the final oxidation of the intermediate 5-formyl-furancarboxylic acid to 2,5-furandicarboxylic acid. Wild-type AAO is not able to catalyze 5-formyl-furancarboxylic acid oxidation Pleurotus eryngii ?
-
-

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.67
-
 5-hydroxymethylfurfural wild-type, pH 6, 25°C Pleurotus eryngii
6.8
-
 5-hydroxymethylfurfural mutant H91N/L170M, pH 6, 25°C Pleurotus eryngii
18.8
-
 5-hydroxymethylfurfural mutant H91N/L170M/F501W, pH 6, 25°C Pleurotus eryngii

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
0.42
-
 5-hydroxymethylfurfural wild-type, pH 6, 25°C Pleurotus eryngii
0.52
-
 5-hydroxymethylfurfural mutant H91N/L170M, pH 6, 25°C Pleurotus eryngii
1.51
-
 5-hydroxymethylfurfural mutant H91N/L170M/F501W, pH 6, 25°C Pleurotus eryngii