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Literature summary for 1.1.3.7 extracted from

  • Jankowski, N.; Koschorreck, K.; Urlacher, V.B.
    High-level expression of aryl-alcohol oxidase 2 from Pleurotus eryngii in Pichia pastoris for production of fragrances and bioactive precursors (2020), Appl. Microbiol. Biotechnol., 104, 9205-9218 .
    View publication on PubMed

Application

Application Comment Organism
synthesis expression and secretion of AAO with yield of 315 mg/l using Pichia pastoris Pleurotus eryngii

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
100000
-
PAGE Pleurotus eryngii

Organism

Organism UniProt Comment Textmining
Pleurotus eryngii D3YBH4
-
-

Posttranslational Modification

Posttranslational Modification Comment Organism
glycoprotein about 60% glycosylation Pleurotus eryngii

Purification (Commentary)

Purification (Comment) Organism
recombinant protein Pleurotus eryngii

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(2E)-hept-2-en-1-ol + O2 31.9% of the activity with benzyl alcohol Pleurotus eryngii (2E)-hept-2-enal + H2O2
-
?
(2E)-hex-2-en-1-ol + O2 63.9% of the activity with benzyl alcohol Pleurotus eryngii (2E)-hex-2-enal + H2O2
-
?
(2E,4E)-hepta-2,4-dien-1-ol + O2 737% of the activity with benzyl alcohol Pleurotus eryngii (2E,4E)-hepta-2,4-dienal + H2O2
-
?
(2E,4E)-hexa-2,4-dien-1-ol + O2 807% of the activity with benzyl alcohol Pleurotus eryngii (2E,4E)-hexa-2,4-dienal + H2O2
-
?
(2H-1,3-benzodioxol-5-yl)methanol + O2 301% of the activity with benzyl alcohol Pleurotus eryngii 2H-1,3-benzodioxole-5-carbaldehyde + H2O2
-
?
(naphthalen-2-yl)methanol + O2 874% of the activity with benzyl alcohol Pleurotus eryngii naphthalene-2-carbaldehyde + H2O2
-
?
(pyrene-1-yl)methanol + O2 35% of the activity with benzyl alcohol Pleurotus eryngii pyrene-1-carbaldehyde + H2O2
-
?
(thiophen-2-yl)methanol + O2 15.8% of the activity with benzyl alcohol Pleurotus eryngii thiophene-2-carbaldehyde + H2O2
-
?
4-aminobenzyl alcohol + O2 18.6% of the activity with benzyl alcohol Pleurotus eryngii 4-aminobenzaldehyde + H2O2
-
?
anisyl alcohol + O2 647% of the activity with benzyl alcohol Pleurotus eryngii anisyl aldehyde + H2O2
-
?
benzyl alcohol + O2
-
Pleurotus eryngii benzaldehyde + H2O2
-
?
cinnamyl alcohol + O2 442% of the activity with benzyl alcohol Pleurotus eryngii cinnamyl aldehyde + H2O2
-
?
cumic alcohol + O2 149% of the activity with benzyl alcohol Pleurotus eryngii cumic aldehyde + H2O2
-
?
isovanillyl alcohol + O2 246% of the activity with benzyl alcohol Pleurotus eryngii isovanillyl aldehyde + H2O2
-
?
veratryl alcohol + O2 322% of the activity with benzyl alcohol Pleurotus eryngii veratryl aldehyde + H2O2
-
?

Subunits

Subunits Comment Organism
monomer 1 * 100000, recombinant protein, 1 * 70000, PNGase F-treated protein, SDS-PAGE, 1 * 60800, calculated from sequence Pleurotus eryngii

Synonyms

Synonyms Comment Organism
AAO2
-
Pleurotus eryngii

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
4 50 1 h, 90% residual activity Pleurotus eryngii
55
-
1 h, 70% residual activity Pleurotus eryngii
60
-
1 h, 10% residual activity Pleurotus eryngii

pH Stability

pH Stability pH Stability Maximum Comment Organism
3 9 1 h, 90% residual activity Pleurotus eryngii