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Literature summary for 1.1.3.7 extracted from

  • Chakraborty, M.; Goel, M.; Chinnadayyala, S.R.; Dahiya, U.R.; Ghosh, S.S.; Goswami, P.
    Molecular characterization and expression of a novel alcohol oxidase from Aspergillus terreus MTCC6324 (2014), PLoS ONE, 9, e95368.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21(DE3) cells Aspergillus terreus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.08
-
4-methoxybenzyl alcohol recombinant enzyme, at pH 6.0 and 30°C Aspergillus terreus
2.88
-
3,4-dimethoxybenzyl alcohol recombinant enzyme, at pH 6.0 and 30°C Aspergillus terreus
4.91
-
3-Methoxybenzyl alcohol recombinant enzyme, at pH 6.0 and 30°C Aspergillus terreus
10.8
-
benzyl alcohol recombinant enzyme, at pH 6.0 and 30°C Aspergillus terreus

Localization

Localization Comment Organism GeneOntology No. Textmining
microsome
-
Aspergillus terreus
-
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
74000
-
-
Aspergillus terreus

Organism

Organism UniProt Comment Textmining
Aspergillus terreus I7FDJ2
-
-
Aspergillus terreus MTCC6324 I7FDJ2
-
-

Purification (Commentary)

Purification (Comment) Organism
nickel Sepharose 6 column chromatography Aspergillus terreus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3,4-dimethoxybenzyl alcohol + O2 i.e. veratryl alcohol Aspergillus terreus 3,4-dimethoxybenzaldehyde + H2O2
-
?
3,4-dimethoxybenzyl alcohol + O2 i.e. veratryl alcohol Aspergillus terreus MTCC6324 3,4-dimethoxybenzaldehyde + H2O2
-
?
3-methoxybenzyl alcohol + O2 i.e. 3-anisyl alcohol Aspergillus terreus 3-methoxybenzaldehyde + H2O2
-
?
3-methoxybenzyl alcohol + O2 i.e. 3-anisyl alcohol Aspergillus terreus MTCC6324 3-methoxybenzaldehyde + H2O2
-
?
4-methoxybenzyl alcohol + O2 i.e. 4-anisyl alcohol, best substrate Aspergillus terreus 4-methoxybenzaldehyde + H2O2
-
?
4-methoxybenzyl alcohol + O2 i.e. 4-anisyl alcohol, best substrate Aspergillus terreus MTCC6324 4-methoxybenzaldehyde + H2O2
-
?
benzyl alcohol + O2 poorest substrate Aspergillus terreus benzaldehyde + H2O2
-
?
benzyl alcohol + O2 poorest substrate Aspergillus terreus MTCC6324 benzaldehyde + H2O2
-
?

Subunits

Subunits Comment Organism
? x * 76000, SDS-PAGE Aspergillus terreus
? x * 74614, MALDI-TOF mass spectrometry Aspergillus terreus

Synonyms

Synonyms Comment Organism
alcohol: O2 oxidoreductase
-
Aspergillus terreus
AOX
-
Aspergillus terreus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
-
-
Aspergillus terreus

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
30 70 the enzyme is stable up to 30°C and the stability is greatly reduced at 40°C (50% inactivation) and leads to 100% inactivation after 5 min at 70°C Aspergillus terreus

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.2
-
benzyl alcohol recombinant enzyme, at pH 6.0 and 30°C Aspergillus terreus
105.7
-
3,4-dimethoxybenzyl alcohol recombinant enzyme, at pH 6.0 and 30°C Aspergillus terreus
115.4
-
3-Methoxybenzyl alcohol recombinant enzyme, at pH 6.0 and 30°C Aspergillus terreus
140.9
-
4-methoxybenzyl alcohol recombinant enzyme, at pH 6.0 and 30°C Aspergillus terreus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6
-
-
Aspergillus terreus

pH Stability

pH Stability pH Stability Maximum Comment Organism
6 9 the recombinant enzyme demonstrates partial pH stability in range from pH 6.0 to pH 9.0 after 1 h Aspergillus terreus

Cofactor

Cofactor Comment Organism Structure
FAD required for activity Aspergillus terreus

pI Value

Organism Comment pI Value Maximum pI Value
Aspergillus terreus isoelectric focusing
-
6.5

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
0.2
-
benzyl alcohol recombinant enzyme, at pH 6.0 and 30°C Aspergillus terreus
23.5
-
3-Methoxybenzyl alcohol recombinant enzyme, at pH 6.0 and 30°C Aspergillus terreus
39.7
-
3,4-dimethoxybenzyl alcohol recombinant enzyme, at pH 6.0 and 30°C Aspergillus terreus
130.5
-
4-methoxybenzyl alcohol at pH 6.0 and 30°C Aspergillus terreus