Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 1.1.3.7 extracted from

  • Tamboli, D.; Telke, A.; Dawkar, V.; Jadhav, S.; Govindwar, S.
    Purification and characterization of bacterial aryl alcohol oxidase from Sphingobacterium sp. ATM and its uses in textile dye decolorization (2011), Biotechnol. Bioprocess Eng., 16, 661-668.
No PubMed abstract available

Inhibitors

Inhibitors Comment Organism Structure
CaCl2 15% inhibition at 0.5 mM Sphingobacterium sp.
dithiothreitol 7% inhibition at 0.5 mM Sphingobacterium sp.
EDTA 24% inhibition at 0.5 mM Sphingobacterium sp.
L-cysteine 20% inhibition at 0.5 mM Sphingobacterium sp.
MgCl2 16% inhibition at 0.5 mM Sphingobacterium sp.
MnCl2 14% inhibition at 0.5 mM Sphingobacterium sp.
additional information no inhibition by CuSO4 at 0.5 mM Sphingobacterium sp.
Sodium azide mixed competitive inhibition, 90% inhibition at 0.5 mM Sphingobacterium sp.
ZnSO4 8% inhibition at 0.5 mM Sphingobacterium sp.

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.162
-
veratryl alcohol pH 3.0, 40°C Sphingobacterium sp.

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
71000
-
x * 71000, SDS-PAGE Sphingobacterium sp.

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
4-methoxybenzyl alcohol + O2 Sphingobacterium sp. i.e. 4-anisyl alcohol 4-methoxybenzaldehyde + H2O2
-
?
4-methoxybenzyl alcohol + O2 Sphingobacterium sp. ATM i.e. 4-anisyl alcohol 4-methoxybenzaldehyde + H2O2
-
?
benzyl alcohol + O2 Sphingobacterium sp.
-
benzaldehyde + H2O2
-
?
benzyl alcohol + O2 Sphingobacterium sp. ATM
-
benzaldehyde + H2O2
-
?
n-propanol + O2 Sphingobacterium sp.
-
propionaldehyde + H2O2
-
?
n-propanol + O2 Sphingobacterium sp. ATM
-
propionaldehyde + H2O2
-
?
veratryl alcohol + O2 Sphingobacterium sp.
-
veratraldehyde + H2O2
-
?

Organism

Organism UniProt Comment Textmining
Sphingobacterium sp.
-
-
-
Sphingobacterium sp. ATM
-
-
-

Purification (Commentary)

Purification (Comment) Organism
native enzyme 24.4fold to homogeneity by anion exchange chromatography and gel filtration Sphingobacterium sp.

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
21.75
-
purified native enzyme, pH 3.0, 40°C Sphingobacterium sp.

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4-methoxybenzyl alcohol + O2 i.e. 4-anisyl alcohol Sphingobacterium sp. 4-methoxybenzaldehyde + H2O2
-
?
4-methoxybenzyl alcohol + O2 i.e. 4-anisyl alcohol Sphingobacterium sp. ATM 4-methoxybenzaldehyde + H2O2
-
?
benzyl alcohol + O2
-
Sphingobacterium sp. benzaldehyde + H2O2
-
?
benzyl alcohol + O2
-
Sphingobacterium sp. ATM benzaldehyde + H2O2
-
?
Direct Red 5B + O2 degradation, dye decolorizing Sphingobacterium sp. 3-diazenyl-7-[(phenylcarbonyl)amino]naphthalene-2-sulfonic acid + H2O2 product identification by GC-MS analysis ?
ethanol + O2 low activity Sphingobacterium sp. acetaldehyde + H2O2
-
?
ethanol + O2 low activity Sphingobacterium sp. ATM acetaldehyde + H2O2
-
?
additional information substrate specificity, overview. No activity with caffeic acid Sphingobacterium sp. ?
-
?
additional information substrate specificity, overview. No activity with caffeic acid Sphingobacterium sp. ATM ?
-
?
n-propanol + O2
-
Sphingobacterium sp. propionaldehyde + H2O2
-
?
n-propanol + O2
-
Sphingobacterium sp. ATM propionaldehyde + H2O2
-
?
veratryl alcohol + O2
-
Sphingobacterium sp. veratraldehyde + H2O2
-
?

Subunits

Subunits Comment Organism
? x * 71000, SDS-PAGE Sphingobacterium sp.

Synonyms

Synonyms Comment Organism
AAO
-
Sphingobacterium sp.

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
40
-
-
Sphingobacterium sp.

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
3
-
-
Sphingobacterium sp.

Cofactor

Cofactor Comment Organism Structure
FAD
-
Sphingobacterium sp.