Literature summary for 1.1.3.6 extracted from

Fazaeli, A.; Golestani, A.; Lakzaei, M.; Rasi Varaei, S.S.; Aminian, M.
Expression optimization, purification, and functional characterization of cholesterol oxidase from Chromobacterium sp. DS1 (2019), PLoS ONE, 14, e0212217 .

Search for more literature for 1.1.3.6

Cloned(Commentary)

Cloned (Comment)	Organism
expression in Escherichia coli	Chromobacterium sp. DS1

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
0.031	-	cholesterol	pH 7.0, 25°C	Chromobacterium sp. DS1

Organism

Organism	UniProt	Comment	Textmining
Chromobacterium sp. DS1	B5MGF8	-	-

Purification (Commentary)

Purification (Comment)	Organism
-	Chromobacterium sp. DS1

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
cholesterol + O2	-	Chromobacterium sp. DS1	cholest-5-en-3-one + H2O2	-	?

Temperature Optimum [°C]

Temperature Optimum [°C]	Temperature Optimum Maximum [°C]	Comment	Organism
70	-	-	Chromobacterium sp. DS1

Temperature Range [°C]

Temperature Minimum [°C]	Temperature Maximum [°C]	Comment	Organism
40	80	40°C: about 50% of maximal activity, 80°C: about 70% of maximal activity	Chromobacterium sp. DS1

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
6	-	-	Chromobacterium sp. DS1

pH Range

pH Minimum	pH Maximum	Comment	Organism
5	8	pH 5.0: about 45% of maximal activity, pH 8.0: about 40% of maximal activity	Chromobacterium sp. DS1

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Release 2023.1 (January 2023)