Application | Comment | Organism |
---|---|---|
diagnostics | the enzyme is an important biotechnological tool for clinical diagnostics. It is also used for tracking intracellular cholesterol. Its utility is limited by the lack of an efficient temporal control of its activity. A rapamycin-inducible fluorescent cholesterol oxidase from Chromobacterium sp. DS-1 (split GFP-COase) is engineered. Induction by rapamycin allows temporal control over enzyme activity and rapidly leads to an efficient reduction of intracellular cholesterol. It is proposed that this drug-inducible split GFP-COase is a new tool to manipulate the cellular cholesterol content and gain insights into cholesterol-dependent proteins and cholesterol-related cell pathologies | Chromobacterium sp. DS-1 |
synthesis | the enzyme is used for the production of steroid drugs and insecticides | Chromobacterium sp. DS-1 |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Chromobacterium sp. DS-1 | B5MGF8 | - |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
cholesterol + O2 | - |
Chromobacterium sp. DS-1 | cholest-5-en-3-one + H2O2 | - |
? |
Synonyms | Comment | Organism |
---|---|---|
COase | - |
Chromobacterium sp. DS-1 |
General Information | Comment | Organism |
---|---|---|
metabolism | the enzyme catalyzes the first step in the biodegradation of cholesterol | Chromobacterium sp. DS-1 |