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Literature summary for 1.1.3.6 extracted from

  • Devi, S.; Sharma, B.; Kumar, R.; Singh Kanwar, S.
    Purification, characterization, and biological cytotoxic activity of the extracellular cholesterol oxidase produced by Castellaniella sp. COX (2020), J. Basic Microbiol., 60, 253-267 .
    View publication on PubMed
Search for more literature for 1.1.3.6

Activating Compound

Activating Compound Comment Organism Structure
ethanol enhances activity Castellaniella sp.
Isopropanol enhances activity Castellaniella sp.

Application

Application Comment Organism
drug development the cytotoxicity activity against a few cancer cell lines, while broadly sparing the normal cells, suggests the possible use of this enzyme as a promising future contender for anticancer therapy Castellaniella sp.

Inhibitors

Inhibitors Comment Organism Structure
Ba2+ 5 mM, about 45% loss of activity Castellaniella sp.
Cu2+ 5 mM, about 40% loss of activity Castellaniella sp.
Fe2+ 5 mM, about 35% loss of activity Castellaniella sp.
Hg2+ 5 mM, about 80% loss of activity Castellaniella sp.
SDS
-
 Castellaniella sp.
Zn2+ 5 mM, about 30% loss of activity Castellaniella sp.

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.16
-
 cholesterol pH 8.0, 40°C Castellaniella sp.

Localization

Localization Comment Organism GeneOntology No. Textmining
extracellular
-
 Castellaniella sp.
-
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
59000
-
 reducing and denaturing SDS-PAGE Castellaniella sp.

Organic Solvent Stability

Organic Solvent Comment Organism
Acetone well tolerated Castellaniella sp.
benzene well tolerated Castellaniella sp.
chloroform well tolerated Castellaniella sp.
dimethyl sulfoxide well tolerated Castellaniella sp.
Methanol well tolerated Castellaniella sp.
n-Butanol well tolerated Castellaniella sp.
toluene well tolerated Castellaniella sp.

Organism

Organism UniProt Comment Textmining
Castellaniella sp.
-
-
-

Purification (Commentary)

Purification (Comment) Organism
salting out and ion-exchange chromatography Castellaniella sp.

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
15
-
 pH 8.0, 40°C Castellaniella sp.

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
cholesterol + O2
-
 Castellaniella sp. cholest-5-en-3-one + H2O2
-
 ?

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
40
-
-
 Castellaniella sp.

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
25 50 25°C: about 70% of maximal activity, 50°C: about 55% of maximal activity Castellaniella sp.

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
37
-
 half-life: 8 h Castellaniella sp.
40
-
 half-life: 7 h Castellaniella sp.
50
-
 half-life: 3 h Castellaniella sp.

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
10.6
-
 cholesterol pH 8.0, 40°C Castellaniella sp.

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
-
 Castellaniella sp.

pH Range

pH Minimum pH Maximum Comment Organism
5 9.5 pH 5.0: about 50% of maximal activity, pH 9.5: about 40% of maximal activity Castellaniella sp.

pH Stability

pH Stability pH Stability Maximum Comment Organism
5
-
 37°C, 3 h, enzyme retains about 60% of its initial activity Castellaniella sp.
6
-
 37°C, 3 h, enzyme retains 76.7% of its initial activity Castellaniella sp.
7
-
 37°C, 3 h, enzyme retains about 85% of its initial activity Castellaniella sp.
8
-
 37°C, 3 h, enzyme retains 86% of its initial activity Castellaniella sp.
9
-
 37°C, 3 h, enzyme retains about 75% of its initial activity Castellaniella sp.
10
-
 37°C, 3 h, enzyme retains about 60% of its initial activity Castellaniella sp.