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Literature summary for 1.1.3.6 extracted from

  • El-Naggar, N.E.; Deraz, S.F.; Soliman, H.M.; El-Deeb, N.M.; El-Shweihy, N.M.
    Purification, characterization and amino acid content of cholesterol oxidase produced by Streptomyces aegyptia NEAE 102 (2017), BMC Microbiol., 17, 76 .
    View publication on PubMedView publication on EuropePMC
Search for more literature for 1.1.3.6

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.152
-
 cholesterol pH 7.0, 37°C Streptomyces aegyptia

Localization

Localization Comment Organism GeneOntology No. Textmining
extracellular
-
 Streptomyces aegyptia
-
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
46000
-
 SDS-PAGE Streptomyces aegyptia

Organism

Organism UniProt Comment Textmining
Streptomyces aegyptia
-
-
-
Streptomyces aegyptia NEAE 102
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Streptomyces aegyptia

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
16.08
-
 pH 7.0, 37°C Streptomyces aegyptia

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
cholesterol + O2
-
 Streptomyces aegyptia cholest-5-en-3-one + H2O2
-
 ?
cholesterol + O2
-
 Streptomyces aegyptia NEAE 102 cholest-5-en-3-one + H2O2
-
 ?

Subunits

Subunits Comment Organism
? x * 46000, SDS-PAGE Streptomyces aegyptia

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
-
 Streptomyces aegyptia

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
25 50 25°C: about % of maximal activity, 50°C: about 60% of maximal activity Streptomyces aegyptia

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
50
-
 half-life: 156.25 min. 63.86% of the initial activity is retained by the enzyme after 20 min of incubation at 50 °C Streptomyces aegyptia
60
-
 half-life: 107.27 min Streptomyces aegyptia
70
-
 half-life: 81.82 min Streptomyces aegyptia
80
-
 half-life: 80.33 min Streptomyces aegyptia

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7
-
-
 Streptomyces aegyptia

pH Range

pH Minimum pH Maximum Comment Organism
6 9 pH 6.0: about 20% of maximal activity, pH 9.0: about 55% of maximal activity Streptomyces aegyptia

pH Stability

pH Stability pH Stability Maximum Comment Organism
5
-
 the enzyme retains 82.36% of its activity after 3 h and losing most of its activity (15.95%) after 24 h Streptomyces aegyptia
7
-
 the remaining activity is 86.96% and 54.48% after 3 h and 24 h Streptomyces aegyptia