Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli. Applying the optimized protocol, the production of recombinant cholesterol oxidase is significantly enhanced from 3.2 to 158 U/l. Under the optimized condition, the enzyme is produced on a large-scale | Streptomyces sp. SA-COO |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.013 | - |
cholesterol | pH 7, 25°C | Streptomyces sp. SA-COO |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
cholesterol + O2 | Streptomyces sp. SA-COO | - |
cholest-5-en-3-one + H2O2 | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Streptomyces sp. SA-COO | P12676 | - |
- |
Purification (Comment) | Organism |
---|---|
column nickel affinity chromatography | Streptomyces sp. SA-COO |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
cholesterol + O2 | - |
Streptomyces sp. SA-COO | cholest-5-en-3-one + H2O2 | - |
? |
Synonyms | Comment | Organism |
---|---|---|
ChoA | - |
Streptomyces sp. SA-COO |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
60 | - |
- |
Streptomyces sp. SA-COO |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | 80 | 30°C: about 42% of maximal activity, 80°C: about 24% of maximal activity | Streptomyces sp. SA-COO |
40 | 70 | the enzyme activity retains more than 60% of its activity at the temperatures from 40°C to 70°C | Streptomyces sp. SA-COO |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6 | 7 | the enzyme activity retains more than 95% of its maximal activity within the pH range of 6-7 at 25°C | Streptomyces sp. SA-COO |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
6 | 7 | the enzyme activity retains more than 95% of its maximal activity within the pH range of 6-7 at 25°C | Streptomyces sp. SA-COO |