KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0962 | - |
cholesterol | enzyme from cultured immobilized cells, pH and temperature not specified in the publication | Streptomyces sp. | |
0.1013 | - |
cholesterol | enzyme from cultured free cells, pH and temperature not specified in the publication | Streptomyces sp. |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
extracellular | - |
Streptomyces sp. | - |
- |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
cholesterol + O2 | Streptomyces sp. | - |
cholest-4-en-3-one + H2O2 | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Streptomyces sp. | - |
- |
- |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
cell culture | production of cholesterol oxidase under batch conditions through Ca-alginate immobilized cells of Streptomyces sp., optimization of immobilization method and production conditions, overview. The enzyme production with immobilized cells is higher in comparison to free cells under optimized conditions. Production medium inoculated with 2% v/v or 8.8 mg dry cell weight seed culture and incubated at 200 rpm and 30°C for 96 h gves maximum COD enzyme production after 96 h whereas the immobilized culture gives maximum production at 37°C and 300 rpm after 72 h of incubation. The immobilized cells can be used for three consecutive fermentation cycles for COD production in higher quantities as compared with free cells | Streptomyces sp. | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
cholesterol + O2 | - |
Streptomyces sp. | cholest-4-en-3-one + H2O2 | - |
? | |
additional information | the enzyme catalyzes the oxidation of steroid substrates which have a hydroxyl group at the 3beta-position of the steroid ring system | Streptomyces sp. | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
3beta-hydroxysteroid: oxygen oxidoreductase | - |
Streptomyces sp. |
COD | - |
Streptomyces sp. |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
FAD | - |
Streptomyces sp. |