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Literature summary for 1.1.3.6 extracted from

  • Niwas, R.; Singh, V.; Singh, R.; Pant, G.; Mitra, K.; Tripathi, C.K.
    Cholesterol oxidase production from entrapped cells of Streptomyces sp. (2014), J. Basic Microbiol., 54, 1233-1239 .
    View publication on PubMed

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0962
-
cholesterol enzyme from cultured immobilized cells, pH and temperature not specified in the publication Streptomyces sp.
0.1013
-
cholesterol enzyme from cultured free cells, pH and temperature not specified in the publication Streptomyces sp.

Localization

Localization Comment Organism GeneOntology No. Textmining
extracellular
-
Streptomyces sp.
-
-

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
cholesterol + O2 Streptomyces sp.
-
cholest-4-en-3-one + H2O2
-
?

Organism

Organism UniProt Comment Textmining
Streptomyces sp.
-
-
-

Source Tissue

Source Tissue Comment Organism Textmining
cell culture production of cholesterol oxidase under batch conditions through Ca-alginate immobilized cells of Streptomyces sp., optimization of immobilization method and production conditions, overview. The enzyme production with immobilized cells is higher in comparison to free cells under optimized conditions. Production medium inoculated with 2% v/v or 8.8 mg dry cell weight seed culture and incubated at 200 rpm and 30°C for 96 h gves maximum COD enzyme production after 96 h whereas the immobilized culture gives maximum production at 37°C and 300 rpm after 72 h of incubation. The immobilized cells can be used for three consecutive fermentation cycles for COD production in higher quantities as compared with free cells Streptomyces sp.
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
cholesterol + O2
-
Streptomyces sp. cholest-4-en-3-one + H2O2
-
?
additional information the enzyme catalyzes the oxidation of steroid substrates which have a hydroxyl group at the 3beta-position of the steroid ring system Streptomyces sp. ?
-
?

Synonyms

Synonyms Comment Organism
3beta-hydroxysteroid: oxygen oxidoreductase
-
Streptomyces sp.
COD
-
Streptomyces sp.

Cofactor

Cofactor Comment Organism Structure
FAD
-
Streptomyces sp.