KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0012 | - |
26-Hydroxycholesterol | - |
Nocardia erythropolis | |
0.0015 | - |
25-hydroxycholesterol | - |
Nocardia erythropolis | |
0.0027 | - |
24-oxocholesterol | - |
Nocardia erythropolis | |
0.0029 | - |
cholesterol | - |
Nocardia erythropolis | |
0.0042 | - |
20-hydroxycholesterol | - |
Nocardia erythropolis | |
0.0043 | - |
24-hydroxycholesterol | - |
Nocardia erythropolis | |
0.0051 | - |
22-hydroxycholesterol | - |
Nocardia erythropolis | |
0.0071 | - |
20,22-dihydroxycholesterol | - |
Nocardia erythropolis |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
cholesterol + O2 | Nocardia erythropolis | - |
cholest-5-en-3-one + H2O2 | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Nocardia erythropolis | - |
- |
- |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
cholesterol + O2 = cholest-5-en-3-one + H2O2 | bifunctional enzyme, catalyzes both the oxidation of DELTA5-ene-3beta hydroxysteroids with a trans A-B ring junction to the corresponding DELTA5-3-ketosteroid with the reduction of oxygen to hydrogen peroxide, and the isomerization to the DELTA4-3-ketosteroid | Nocardia erythropolis |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
20,22-dihydroxycholesterol + O2 | - |
Nocardia erythropolis | 20,22-dihydroxycholest-5-en-3-one + H2O2 | - |
? | |
20-hydroxycholesterol + O2 | - |
Nocardia erythropolis | 20-hydroxycholest-5-en-3-one + H2O2 | - |
? | |
22-hydroxycholesterol + O2 | - |
Nocardia erythropolis | 22-hydroxycholest-5-en-3-one + H2O2 | - |
? | |
24-hydroxycholesterol + O2 | - |
Nocardia erythropolis | 24-hydroxycholest-5-en-3-one + H2O2 | - |
? | |
24-oxocholesterol + O2 | - |
Nocardia erythropolis | 24-oxocholest-5-en-3-one + H2O2 | - |
? | |
25-hydroxycholesterol + O2 | - |
Nocardia erythropolis | 25-hydroxycholest-5-en-3-one + H2O2 | - |
? | |
26-hydroxycholesterol + O2 | - |
Nocardia erythropolis | 26-hydroxycholest-5-en-3-one + H2O2 | - |
? | |
cholesterol + O2 | overview: substrate specificity | Nocardia erythropolis | cholest-4-en-3-one + H2O2 | - |
? | |
cholesterol + O2 | relatively unreactive 17keto-DELTA5-3beta-hydroxysteroids are converted to satisfactory substrates by formation of isopentyloximes or benzyloximes | Nocardia erythropolis | cholest-4-en-3-one + H2O2 | - |
? | |
cholesterol + O2 | size and shape of steroid 17beta-side-chain and oxygenation of nucleus | Nocardia erythropolis | cholest-4-en-3-one + H2O2 | - |
? | |
cholesterol + O2 | - |
Nocardia erythropolis | cholest-5-en-3-one + H2O2 | - |
? |