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Literature summary for 1.1.3.6 extracted from

  • Lee, S.; Rhee, H.; Tae, W.; Shin, J.; Park, B.
    Purification and characterization of cholesterol oxidase from Pseudomonas sp. and taxonomic study of the strain (1989), Appl. Microbiol. Biotechnol., 31, 542-546.
No PubMed abstract available
Search for more literature for 1.1.3.6

Inhibitors

Inhibitors Comment Organism Structure
Fe2+
-
 Pseudomonas sp.
Hg2+
-
 Pseudomonas sp.
Zn2+
-
 Pseudomonas sp.

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.2
-
 cholesterol
-
 Pseudomonas sp.

Metals/Ions

Metals/Ions Comment Organism Structure
Mn2+
-
 Pseudomonas sp.

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
56000
-
 SDS-PAGE, gel filtration Pseudomonas sp.

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
cholesterol + O2 Pseudomonas sp.
-
 cholest-5-en-3-one + H2O2
-
 ?

Organism

Organism UniProt Comment Textmining
Pseudomonas sp.
-
-
-

Reaction

Reaction Comment Organism Reaction ID
cholesterol + O2 = cholest-5-en-3-one + H2O2 bifunctional enzyme, catalyzes both the oxidation of DELTA5-ene-3beta hydroxysteroids with a trans A-B ring junction to the corresponding DELTA5-3-ketosteroid with the reduction of oxygen to hydrogen peroxide, and the isomerization to the DELTA4-3-ketosteroid Pseudomonas sp.
a

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
27.9
-
-
 Pseudomonas sp.

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
5alpha-cholestan-3beta-ol + O2
-
 Pseudomonas sp. 5alpha-cholestan-3-one + H2O2
-
 ?
cholesterol + O2 enzyme specifically oxidizes 3beta-hydroxy groups in steroids Pseudomonas sp. cholest-4-en-3-one + H2O2
-
 ?
cholesterol + O2
-
 Pseudomonas sp. cholest-5-en-3-one + H2O2
-
 ?
dihydrocholesterol + O2
-
 Pseudomonas sp. 5alpha-cholestan-3-one + H2O2
-
 ?
ergosterol + O2
-
 Pseudomonas sp. dehydroergosterol + H2O2
-
 ?
stigmastanol + O2
-
 Pseudomonas sp. stigmastanone + H2O2
-
 ?
stigmasterol + O2
-
 Pseudomonas sp. stigmasta-5,22-dien-3-one + H2O2
-
 ?

Subunits

Subunits Comment Organism
monomer 1 * 56000, SDS-PAGE Pseudomonas sp.

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
70
-
 5 min, no loss of activity Pseudomonas sp.
70
-
 30 min, 15% loss of activity Pseudomonas sp.

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7
-
-
 Pseudomonas sp.

pH Range

pH Minimum pH Maximum Comment Organism
5 9 33% of maximal activity at pH 5.0 and pH 9.0 Pseudomonas sp.

pH Stability

pH Stability pH Stability Maximum Comment Organism
5 8 60°C, 1 min, stable Pseudomonas sp.
10
-
 60°C, 1 min, 25% loss of activity Pseudomonas sp.

Cofactor

Cofactor Comment Organism Structure
FAD exogenous addition increases activity Pseudomonas sp.