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Literature summary for 1.1.3.50 extracted from

  • Kumano, T.; Hori, S.; Watanabe, S.; Terashita, Y.; Yu, H.Y.; Hashimoto, Y.; Senda, T.; Senda, M.; Kobayashi, M.
    FAD-dependent C-glycoside-metabolizing enzymes in microorganisms Screening, characterization, and crystal structure analysis (2021), Proc. Natl. Acad. Sci. USA, 118, e2106580118 .
    View publication on PubMedView publication on EuropePMC
Search for more literature for 1.1.3.50

Crystallization (Commentary)

Crystallization (Comment) Organism
hanging-drop vapor-diffusion method Microbacterium sp. 5-2b

Protein Variants

Protein Variants Comment Organism
H429A inactive mutant enzyme Microbacterium sp. 5-2b

Inhibitors

Inhibitors Comment Organism Structure
(NH4)2S2O8 1 mM, 50% inhibition Microbacterium sp. 5-2b
Al2+ 1 mM, 88% inhibition Microbacterium sp. 5-2b
aminoguanidine 1 mM, 9% inhibition Microbacterium sp. 5-2b
Ca2+ 1 mM, 64% inhibition Microbacterium sp. 5-2b
carminate substrate inhibition Microbacterium sp. 5-2b
Co2+ 1 mM, 72% inhibition Microbacterium sp. 5-2b
Cu2+ 1 mM, 86% inhibition Microbacterium sp. 5-2b
diethyldithiocarbamate 1 mM, 11% inhibition Microbacterium sp. 5-2b
Fe2+ 1 mM, complete inhibition Microbacterium sp. 5-2b
Fe3+ 1 mM, 54% inhibition Microbacterium sp. 5-2b
Hg2+ 1 mM, complete inhibition Microbacterium sp. 5-2b
KCN 1 mM, 23% inhibition Microbacterium sp. 5-2b
additional information 5,5'-dithio-bis-2-nitrobenzoate, N-ethylmaleimide, and iodoacetate have no significant effect on activity Microbacterium sp. 5-2b
NaN3 1 mM, 12% inhibition Microbacterium sp. 5-2b
p-chloromercuribenzoate 1 mM, 58% inhibition Microbacterium sp. 5-2b
Pb2+ 1 mM, 42% inhibition Microbacterium sp. 5-2b
phenazine methosufate 1 mM, 9% inhibition Microbacterium sp. 5-2b
phenylhydrazine 1 mM, 11% inhibition Microbacterium sp. 5-2b
Sr2+ 1 mM, 89% inhibition Microbacterium sp. 5-2b
Zn2+ 1 mM, 74% inhibition Microbacterium sp. 5-2b

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.019
-
 carminate pH 8.0, 28°C Microbacterium sp. 5-2b

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
54900
-
 gel filtration Microbacterium sp. 5-2b

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
carminate + O2 Microbacterium sp. 5-2b initial enzyme for carminic acid metabolism 3'-dehydrocarminate + H2O2
-
 ?

Organism

Organism UniProt Comment Textmining
Microbacterium sp. 5-2b BBE36490
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Microbacterium sp. 5-2b

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
19.9
-
 pH 8.0, 28°C Microbacterium sp. 5-2b

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
carminate + O2
-
 Microbacterium sp. 5-2b 3'-dehydrocarminate + H2O2
-
 ?
carminate + O2 initial enzyme for carminic acid metabolism Microbacterium sp. 5-2b 3'-dehydrocarminate + H2O2
-
 ?
homoorientin + O2
-
 Microbacterium sp. 5-2b 3'-keto-homoorientin + H2O2
-
 ?
isovitexin + O2
-
 Microbacterium sp. 5-2b 3'-keto-isovitexin + H2O2
-
 ?
mangiferin + O2
-
 Microbacterium sp. 5-2b 3'-keto-magniferin + H2O2
-
 ?
additional information the enzyme alsoshows broad substrate specificities toward O-glycosides, the specific activities are 100 to 1000 times lower than those of C-glycosides. No activity with D-glucose or C8-glycosylated flavonoids Microbacterium sp. 5-2b ?
-
-

Subunits

Subunits Comment Organism
monomer 1 * 50000, SDS-PAGE Microbacterium sp. 5-2b
monomer 1 * 52300, calculated from sequence Microbacterium sp. 5-2b

Synonyms

Synonyms Comment Organism
C-glycoside 3-oxidase
-
 Microbacterium sp. 5-2b
CarA
-
 Microbacterium sp. 5-2b

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30 40
-
 Microbacterium sp. 5-2b

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
20 50 20°C: about 20% of maximal activity, 50°C: about 15% of maximal activity Microbacterium sp. 5-2b

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
30
-
 pH 8.0, 10 min, stable Microbacterium sp. 5-2b

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
4.3
-
 carminate pH 8.0, 28°C Microbacterium sp. 5-2b

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.6 8.4
-
 Microbacterium sp. 5-2b

pH Range

pH Minimum pH Maximum Comment Organism
6.5 10 pH 6.5: about 75% of maximal activity, pH 10.0: about 50% of maximal activity Microbacterium sp. 5-2b

pH Stability

pH Stability pH Stability Maximum Comment Organism
4.2 11.4 28°C, 10 min, stable Microbacterium sp. 5-2b

Cofactor

Cofactor Comment Organism Structure
FAD flavoprotein. One FAD would bind one CarA monomer Microbacterium sp. 5-2b

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.7
-
 carminate pH 8.0, 28°C Microbacterium sp. 5-2b

General Information

General Information Comment Organism
metabolism the enzyme plays a crucial role in the metabolism of C-glycosides Microbacterium sp. 5-2b