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Literature summary for 1.1.3.47 extracted from

  • Dijkman, W.; Fraaije, M.
    Discovery and characterization of a 5-hydroxymethylfurfural oxidase from Methylovorus sp. strain MP688 (2014), Appl. Environ. Microbiol., 80, 1082-1090.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 1.1.3.47

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Methylovorus sp. MP688

Protein Variants

Protein Variants Comment Organism
H467A catalysis of the mutant enzyme is severely reduced compared to that of the wild-type enzyme. With vanillyl alcohol as the substrate, the kcat value of the mutant enzyme is 4400 times lower than that of the wild-type enzyme Methylovorus sp. MP688

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.4
-
 5-(hydroxymethyl)furfural pH 8.0, 25°C Methylovorus sp. MP688

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
70000
-
 x * 70000, SDS-PAGE Methylovorus sp. MP688

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
5-(hydroxymethyl)furfural + 3 O2 + 2 H2O Methylovorus sp. MP688 the enzyme is involved in the degradation and detoxification of 5-(hydroxymethyl)furfural furan-2,5-dicarboxylate + 3 H2O2
-
 ?

Organism

Organism UniProt Comment Textmining
Methylovorus sp. MP688 E4QP00
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Methylovorus sp. MP688

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
5-(dihydroxymethyl)furan-2-carbaldehyde + O2
-
 Methylovorus sp. MP688 5-formylfuran-2-carboxylate + H2O2 spontaneous formation of 5-(dihydroxymethyl)furan-2-carboxylate from 5-formylfuran-2-carboxylate ?
5-(dihydroxymethyl)furan-2-carboxylate + O2
-
 Methylovorus sp. MP688 furan-2,5-dicarboxylate + H2O2
-
 ?
5-(hydroxymethyl)furfural + 3 O2 + 2 H2O overall reaction Methylovorus sp. MP688 furan-2,5-dicarboxylate + 3 H2O2
-
 ?
5-(hydroxymethyl)furfural + 3 O2 + 2 H2O the enzyme is involved in the degradation and detoxification of 5-(hydroxymethyl)furfural Methylovorus sp. MP688 furan-2,5-dicarboxylate + 3 H2O2
-
 ?
5-(hydroxymethyl)furfural + O2
-
 Methylovorus sp. MP688 furan-2,5-dicarbaldehyde + H2O2 spontaneous formation of 5-(dihydroxymethyl)furan-2-carbaldehyde from furan-2,5-dicarbaldehyde ?
additional information the enzyme has a broad substrate range that overlaps with EC 1.1.3.7, aryl-alcohol oxidase Methylovorus sp. MP688 ?
-
 ?

Subunits

Subunits Comment Organism
? x * 70000, SDS-PAGE Methylovorus sp. MP688

Synonyms

Synonyms Comment Organism
5-hydroxymethylfurfural oxidase
-
 Methylovorus sp. MP688
HMF oxidase
-
 Methylovorus sp. MP688
HMFO
-
 Methylovorus sp. MP688

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
25
-
 assay at Methylovorus sp. MP688

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
9.9
-
 5-(hydroxymethyl)furfural pH 8.0, 25°C Methylovorus sp. MP688

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
 assay at Methylovorus sp. MP688

Cofactor

Cofactor Comment Organism Structure
flavin the flavin cofactor is dissociable Methylovorus sp. MP688

General Information

General Information Comment Organism
metabolism the enzyme is involved in the degradation and detoxification of 5-(hydroxymethyl)furfural Methylovorus sp. MP688

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
7.1
-
 5-(hydroxymethyl)furfural pH 8.0, 25°C Methylovorus sp. MP688