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Literature summary for 1.1.3.42 extracted from

  • Katayama, K.; Kobayashi, T.; Oikawa, H.; Honma, M.; Ichihara, A.
    Enzymatic activity and partial purification of solanapyrone synthase: First enzyme catalyzing Diels-Alder reaction (1998), Biochim. Biophys. Acta, 1384, 387-395.
    View publication on PubMed
Search for more literature for 1.1.3.42

General Stability

General Stability Organism
solanapyrone synthase activity is rather unstable in both crude and partially purified states. It loses 55% of its activity after 36 h at 4°C but the addition of 30% glycerol markedly improves stability, the preparation retains 55% activity for 9 days at 4°C. Other additives such as EDTA, thiol reagents and reducing agents are not effective Alternaria solani

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.016
-
 prosolanapyrone II in 50 mM potassium phosphate buffer, pH 7.0, at 30°C Alternaria solani

Metals/Ions

Metals/Ions Comment Organism Structure
additional information the activity of solanapyrone synthase is not affected by divalent cations Alternaria solani

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
46000 62000 native enzyme, gel filtration Alternaria solani

Organism

Organism UniProt Comment Textmining
Alternaria solani
-
-
-

Purification (Commentary)

Purification (Comment) Organism
partial purification by ammonium sulfate precipitation and DEAE-Sepharose column chromatography Alternaria solani

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.00084
-
 crude extract, in 50 mM potassium phosphate buffer, pH 7.0, at 30°C Alternaria solani
0.0206
-
 after 25fold purification, in 50 mM potassium phosphate buffer, pH 7.0, at 30°C Alternaria solani

Storage Stability

Storage Stability Organism
4°C, crude and partially purified states in the presence of 30% glycerol, 9 days, 45% loss of activity Alternaria solani
4°C, crude and partially purified states, 36 h, 55% loss of activity Alternaria solani

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
prosolanapyrone II + O2
-
 Alternaria solani prosolanapyrone III + H2O2
-
 r

Synonyms

Synonyms Comment Organism
solanapyrone synthase
-
 Alternaria solani
SPS
-
 Alternaria solani

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
5.5
-
 in sodium acetate buffer Alternaria solani
7
-
 in potassium phosphate buffer Alternaria solani
7.5
-
 in Tris buffer Alternaria solani

pH Stability

pH Stability pH Stability Maximum Comment Organism
3.7
-
 irreversible inactivation is observed below pH 3.7 Alternaria solani

Cofactor

Cofactor Comment Organism Structure
additional information the activity of solanapyrone synthase is not affected by NAD+, NADP+, FMN, and FAD+ Alternaria solani