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Literature summary for 1.1.3.41 extracted from

  • Chen, Z.; Li, Z.; Li, F.; Wang, N.; Gao, X.D.
    Characterization of alditol oxidase from Streptomyces coelicolor and its application in the production of rare sugars (2020), Bioorg. Med. Chem., 28, 115464 .
    View publication on PubMed
Search for more literature for 1.1.3.41

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Streptomyces coelicolor

Protein Variants

Protein Variants Comment Organism
synthesis synthesis of rare sugars using Escherichia coli whole cells. The donor substrate dihydroxyacetone phosphate (DHAP) is generated from glycerol by glycerol kinase (GK) and glycerol phosphate oxidase (GPO). The acceptor D-glyceraldehyde is directly produced from glycerol by alditol oxidase. The aldol reaction between DHAP and D-glyceraldehyde is performed by L-rhamnulose-1-phosphate aldolase (RhaD) to generate the corresponding sugar-1-phosphate. Finally, the phosphate group is removed by fructose-1-phosphatase (YqaB) to obtain the rare sugars D-sorbose and D-psicose. Under the optimized conditions, the cascade produces 7.9 g/l of D-sorbose and D-psicose with a total conversion rate of 17.7% from glycerol Streptomyces coelicolor

Organism

Organism UniProt Comment Textmining
Streptomyces coelicolor
-
-
-
Streptomyces coelicolor A3(2)
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
glycerol + O2
-
 Streptomyces coelicolor D-glyceraldehyde + H2O2
-
 ?
glycerol + O2
-
 Streptomyces coelicolor A3(2) D-glyceraldehyde + H2O2
-
 ?

Synonyms

Synonyms Comment Organism
AldO
-
 Streptomyces coelicolor

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
35
-
-
 Streptomyces coelicolor

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5
-
-
 Streptomyces coelicolor