Cloned (Comment) | Organism |
---|---|
functional expression of recombinant oxidase-peroxidase fusion mutant enzyme in Escherichia coli strain BL21(DE3) periplasm | Streptomyces coelicolor |
Protein Variants | Comment | Organism |
---|---|---|
additional information | construction of an AldO mutant synthetic bifunctional enzyme, the enzyme from Streptomyces coelicolor A3(2) is endowed with an extra catalytic functionality by fusing it to a microperoxidase. The mutant is functional and a both fully covalently flavinylated and heminylated: an oxiperoxidase. Replacement of portions of the wild-type AldO sequence with the bacterial cytochrome c CXXCH heme-binding motif, domain structure, overview | Streptomyces coelicolor |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.29 | 0.35 | xylitol | recombinant oxidase-peroxidase fusion mutant enzyme, pH 7.5, temperature not specified in the publication | Streptomyces coelicolor |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
50000 | - |
x * 50000, recombinant oxidase-peroxidase fusion mutant enzyme, SDS-PAGE | Streptomyces coelicolor |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
xylitol + O2 | Streptomyces coelicolor | - |
xylose + H2O2 | - |
? | |
xylitol + O2 | Streptomyces coelicolor A3(2) | - |
xylose + H2O2 | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Streptomyces coelicolor | Q9ZBU1 | - |
- |
Streptomyces coelicolor A3(2) | Q9ZBU1 | - |
- |
Purification (Comment) | Organism |
---|---|
recombinant oxidase-peroxidase fusion mutant enzyme from Escherichia coli strain BL21(DE3) | Streptomyces coelicolor |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
xylitol + O2 | - |
Streptomyces coelicolor | xylose + H2O2 | - |
? | |
xylitol + O2 | - |
Streptomyces coelicolor A3(2) | xylose + H2O2 | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 50000, recombinant oxidase-peroxidase fusion mutant enzyme, SDS-PAGE | Streptomyces coelicolor |
Synonyms | Comment | Organism |
---|---|---|
AldO | - |
Streptomyces coelicolor |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
9.1 | 12.2 | xylitol | recombinant oxidase-peroxidase fusion mutant enzyme, pH 7.5, temperature not specified in the publication | Streptomyces coelicolor |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
FAD | - |
Streptomyces coelicolor |