Cloned (Comment) | Organism |
---|---|
DNA and amino acid sequence determination and analysis, sequence comparison, expression of His6-tagged enzyme in Escherichia coli strain MC1061 | Streptomyces coelicolor |
gene aldO, DNA and amino acid sequence determination and analysis, sequence comparison, expression of His6-tagged enzyme in Escherichia coli strain BL21(DE3) | Acidothermus cellulolyticus |
Protein Variants | Comment | Organism |
---|---|---|
E154P | site-directed mutagenesis, the mutant shows increased thermolability compared to the wild-type enzyme | Acidothermus cellulolyticus |
E50P | site-directed mutagenesis, the mutant shows increased thermolability compared to the wild-type enzyme | Acidothermus cellulolyticus |
K18R/E19R | site-directed mutagenesis, the mutant shows increased thermolability compared to the wild-type enzyme | Acidothermus cellulolyticus |
L234R/D235P | site-directed mutagenesis, the mutant shows increased thermolability compared to the wild-type enzyme | Acidothermus cellulolyticus |
R232A/P233G | site-directed mutagenesis, the mutant shows increased thermolability compared to the wild-type enzyme | Acidothermus cellulolyticus |
R232A/P233G/L234R/D235P | site-directed mutagenesis, the mutant shows increased thermolability compared to the wild-type enzyme | Acidothermus cellulolyticus |
T16R/A17P | site-directed mutagenesis, the mutant shows increased thermolability compared to the wild-type enzyme | Acidothermus cellulolyticus |
T16R/A17P/K18R/G19R | site-directed mutagenesis, the mutant shows increased thermolability compared to the wild-type enzyme | Acidothermus cellulolyticus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | steady-state kinetic analysis, overview | Streptomyces coelicolor | |
additional information | - |
additional information | steady-state kinetic analysis, overview | Acidothermus cellulolyticus | |
0.007 | - |
xylitol | pH 7.5, 25°C, recombinant enzyme | Acidothermus cellulolyticus | |
0.32 | - |
xylitol | pH 7.5, 25°C, recombinant enzyme | Streptomyces coelicolor | |
0.44 | - |
D-sorbitol | pH 7.5, 25°C, recombinant enzyme | Acidothermus cellulolyticus | |
1.4 | - |
D-sorbitol | pH 7.5, 25°C, recombinant enzyme | Streptomyces coelicolor | |
5.5 | - |
D-mannitol | pH 7.5, 25°C, recombinant enzyme | Acidothermus cellulolyticus | |
18 | - |
(R)-1-phenyl-1,2-ethanediol | pH 7.5, 25°C, recombinant enzyme | Acidothermus cellulolyticus | |
27 | - |
(S)-1-phenyl-1,2-ethanediol | pH 7.5, 25°C, recombinant enzyme | Acidothermus cellulolyticus | |
36 | - |
D-mannitol | pH 7.5, 25°C, recombinant enzyme | Streptomyces coelicolor | |
68 | - |
1-phenyl-1,2-ethanediol | pH 7.5, 25°C, recombinant enzyme | Acidothermus cellulolyticus | |
83 | - |
1-phenyl-1,2-ethanediol | pH 7.5, 25°C, recombinant enzyme | Streptomyces coelicolor | |
86 | - |
(S)-1-phenyl-1,2-ethanediol | pH 7.5, 25°C, recombinant enzyme | Streptomyces coelicolor | |
101 | - |
(R)-1-phenyl-1,2-ethanediol | pH 7.5, 25°C, recombinant enzyme | Streptomyces coelicolor | |
270 | - |
glycerol | pH 7.5, 25°C, recombinant enzyme | Acidothermus cellulolyticus | |
350 | - |
glycerol | pH 7.5, 25°C, recombinant enzyme | Streptomyces coelicolor |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
46800 | - |
about, sequence calculation | Acidothermus cellulolyticus |
47000 | - |
1 * 47000, SDS-PAGE | Streptomyces coelicolor |
47000 | - |
1 * 47000, SDS-PAGE, 1 * 46800, about, sequence calculation | Acidothermus cellulolyticus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Acidothermus cellulolyticus | A0LST6 | - |
- |
Acidothermus cellulolyticus 11B | A0LST6 | - |
- |
Streptomyces coelicolor | Q9ZBU1 | - |
- |
Streptomyces coelicolor A3(2) | Q9ZBU1 | - |
- |
Purification (Comment) | Organism |
---|---|
recombinant His6-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography, factor Xa protease cleavage of the His-tag | Acidothermus cellulolyticus |
recombinant His6-tagged enzyme from Escherichia coli strain MC1061 | Streptomyces coelicolor |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
(R)-1-phenyl-1,2-ethanediol + O2 | - |
Streptomyces coelicolor | ? + H2O2 | - |
? | |
(R)-1-phenyl-1,2-ethanediol + O2 | - |
Acidothermus cellulolyticus | ? + H2O2 | - |
? | |
(R)-1-phenyl-1,2-ethanediol + O2 | - |
Acidothermus cellulolyticus 11B | ? + H2O2 | - |
? | |
(R)-1-phenyl-1,2-ethanediol + O2 | - |
Streptomyces coelicolor A3(2) | ? + H2O2 | - |
? | |
(S)-1-phenyl-1,2-ethanediol + O2 | - |
Streptomyces coelicolor | ? + H2O2 | - |
? | |
(S)-1-phenyl-1,2-ethanediol + O2 | - |
Acidothermus cellulolyticus | ? + H2O2 | - |
? | |
(S)-1-phenyl-1,2-ethanediol + O2 | - |
Acidothermus cellulolyticus 11B | ? + H2O2 | - |
? | |
(S)-1-phenyl-1,2-ethanediol + O2 | - |
Streptomyces coelicolor A3(2) | ? + H2O2 | - |
? | |
1-phenyl-1,2-ethanediol + O2 | - |
Streptomyces coelicolor | ? + H2O2 | - |
? | |
1-phenyl-1,2-ethanediol + O2 | - |
Acidothermus cellulolyticus | ? + H2O2 | - |
? | |
1-phenyl-1,2-ethanediol + O2 | - |
Acidothermus cellulolyticus 11B | ? + H2O2 | - |
? | |
1-phenyl-1,2-ethanediol + O2 | - |
Streptomyces coelicolor A3(2) | ? + H2O2 | - |
? | |
D-mannitol + O2 | - |
Streptomyces coelicolor | ? + H2O2 | - |
? | |
D-mannitol + O2 | - |
Acidothermus cellulolyticus | ? + H2O2 | - |
? | |
D-sorbitol + O2 | - |
Streptomyces coelicolor | ? + H2O2 | - |
? | |
D-sorbitol + O2 | - |
Acidothermus cellulolyticus | ? + H2O2 | - |
? | |
glycerol + O2 | - |
Streptomyces coelicolor | ? + H2O2 | - |
? | |
glycerol + O2 | - |
Acidothermus cellulolyticus | ? + H2O2 | - |
? | |
glycerol + O2 | - |
Acidothermus cellulolyticus 11B | ? + H2O2 | - |
? | |
glycerol + O2 | - |
Streptomyces coelicolor A3(2) | ? + H2O2 | - |
? | |
xylitol + O2 | - |
Streptomyces coelicolor | xylose + H2O2 | - |
? | |
xylitol + O2 | - |
Acidothermus cellulolyticus | xylose + H2O2 | - |
? | |
xylitol + O2 | - |
Acidothermus cellulolyticus 11B | xylose + H2O2 | - |
? | |
xylitol + O2 | - |
Streptomyces coelicolor A3(2) | xylose + H2O2 | - |
? |
Subunits | Comment | Organism |
---|---|---|
monomer | 1 * 47000, SDS-PAGE | Streptomyces coelicolor |
monomer | 1 * 47000, SDS-PAGE, 1 * 46800, about, sequence calculation | Acidothermus cellulolyticus |
Synonyms | Comment | Organism |
---|---|---|
AldO | - |
Streptomyces coelicolor |
AldO | - |
Acidothermus cellulolyticus |
HotAldO | - |
Streptomyces coelicolor |
HotAldO | - |
Acidothermus cellulolyticus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
25 | - |
assay at | Streptomyces coelicolor |
25 | - |
assay at | Acidothermus cellulolyticus |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
25 | 60 | inactivation at 60°C and 50% remaining activity at 55°C | Streptomyces coelicolor |
25 | 84 | AldO is a highly thermostable enzyme with an unfolding temperature of 84°C, inactivation above, and an activity half-life at 75°C of 112 min | Acidothermus cellulolyticus |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0004 | - |
(S)-1-phenyl-1,2-ethanediol | pH 7.5, 25°C, recombinant enzyme | Acidothermus cellulolyticus | |
0.008 | - |
(S)-1-phenyl-1,2-ethanediol | pH 7.5, 25°C, recombinant enzyme | Streptomyces coelicolor | |
0.1 | - |
(R)-1-phenyl-1,2-ethanediol | pH 7.5, 25°C, recombinant enzyme | Acidothermus cellulolyticus | |
0.1 | - |
1-phenyl-1,2-ethanediol | pH 7.5, 25°C, recombinant enzyme | Acidothermus cellulolyticus | |
0.36 | - |
1-phenyl-1,2-ethanediol | pH 7.5, 25°C, recombinant enzyme | Streptomyces coelicolor | |
0.74 | - |
(R)-1-phenyl-1,2-ethanediol | pH 7.5, 25°C, recombinant enzyme | Streptomyces coelicolor | |
1.2 | - |
D-sorbitol | pH 7.5, 25°C, recombinant enzyme | Acidothermus cellulolyticus | |
1.3 | - |
glycerol | pH 7.5, 25°C, recombinant enzyme | Acidothermus cellulolyticus | |
1.6 | - |
glycerol | pH 7.5, 25°C, recombinant enzyme | Streptomyces coelicolor | |
1.9 | - |
xylitol | pH 7.5, 25°C, recombinant enzyme | Acidothermus cellulolyticus | |
2.5 | - |
D-mannitol | pH 7.5, 25°C, recombinant enzyme | Acidothermus cellulolyticus | |
9.2 | - |
D-mannitol | pH 7.5, 25°C, recombinant enzyme | Streptomyces coelicolor | |
13 | - |
xylitol | pH 7.5, 25°C, recombinant enzyme | Streptomyces coelicolor | |
17 | - |
D-sorbitol | pH 7.5, 25°C, recombinant enzyme | Streptomyces coelicolor |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
assay at | Streptomyces coelicolor |
7.5 | - |
assay at | Acidothermus cellulolyticus |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
FAD | covalently bound, flavoenzyme | Streptomyces coelicolor | |
FAD | covalently bound, flavoenzyme | Acidothermus cellulolyticus |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.00001 | - |
(S)-1-phenyl-1,2-ethanediol | pH 7.5, 25°C, recombinant enzyme | Acidothermus cellulolyticus | |
0.0001 | - |
(S)-1-phenyl-1,2-ethanediol | pH 7.5, 25°C, recombinant enzyme | Streptomyces coelicolor | |
0.0015 | - |
1-phenyl-1,2-ethanediol | pH 7.5, 25°C, recombinant enzyme | Acidothermus cellulolyticus | |
0.0043 | - |
1-phenyl-1,2-ethanediol | pH 7.5, 25°C, recombinant enzyme | Streptomyces coelicolor | |
0.0046 | - |
glycerol | pH 7.5, 25°C, recombinant enzyme | Streptomyces coelicolor | |
0.0048 | - |
glycerol | pH 7.5, 25°C, recombinant enzyme | Acidothermus cellulolyticus | |
0.0056 | - |
(R)-1-phenyl-1,2-ethanediol | pH 7.5, 25°C, recombinant enzyme | Acidothermus cellulolyticus | |
0.0073 | - |
(R)-1-phenyl-1,2-ethanediol | pH 7.5, 25°C, recombinant enzyme | Streptomyces coelicolor | |
0.26 | - |
D-mannitol | pH 7.5, 25°C, recombinant enzyme | Streptomyces coelicolor | |
0.45 | - |
D-mannitol | pH 7.5, 25°C, recombinant enzyme | Acidothermus cellulolyticus | |
2.7 | - |
D-sorbitol | pH 7.5, 25°C, recombinant enzyme | Acidothermus cellulolyticus | |
12 | - |
D-sorbitol | pH 7.5, 25°C, recombinant enzyme | Streptomyces coelicolor | |
27 | - |
xylitol | pH 7.5, 25°C, recombinant enzyme | Acidothermus cellulolyticus | |
41 | - |
xylitol | pH 7.5, 25°C, recombinant enzyme | Streptomyces coelicolor |