Application | Comment | Organism |
---|---|---|
synthesis | AldO is an enantioselective biocatalyst for the kinetic resolution of racemic 1,2-diols | Streptomyces coelicolor |
Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli Top10 cells | Streptomyces coelicolor |
General Stability | Organism |
---|---|
under conditions which are applied for the oxidation experiments (10 mM of aldoitol oxidase in 50 mM sodium phosphate buffer, pH 7.5, at 30°C and rotary shaking (100 rpm)) the enzyme has a half-life of 30 h | Streptomyces coelicolor |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | substrate specificity and steady state kinetics, overview | Streptomyces coelicolor | |
0.32 | - |
xylitol | pH 7.5, 30°C | Streptomyces coelicolor | |
0.32 | - |
xylitol | in 50 mM sodium phosphate buffer, at pH 7.5 and 30°C | Streptomyces coelicolor | |
1.4 | - |
D-sorbitol | pH 7.5, 30°C | Streptomyces coelicolor | |
1.4 | - |
D-sorbitol | in 50 mM sodium phosphate buffer, at pH 7.5 and 30°C | Streptomyces coelicolor | |
10 | - |
(R)-1-phenyl-1,2-ethanediol | pH 7.5, 30°C | Streptomyces coelicolor | |
25 | - |
L-Threitol | pH 7.5, 30°C | Streptomyces coelicolor | |
25 | - |
L-Threitol | in 50 mM sodium phosphate buffer, at pH 7.5 and 30°C | Streptomyces coelicolor | |
35 | - |
2-amino-1-pentanol | pH 7.5, 30°C | Streptomyces coelicolor | |
35 | - |
2-amino-1-pentanol | in 50 mM sodium phosphate buffer, at pH 7.5 and 30°C | Streptomyces coelicolor | |
36 | - |
D-mannitol | pH 7.5, 30°C | Streptomyces coelicolor | |
36 | - |
D-mannitol | in 50 mM sodium phosphate buffer, at pH 7.5 and 30°C | Streptomyces coelicolor | |
42 | - |
4-pentene-1,2-diol | pH 7.5, 30°C | Streptomyces coelicolor | |
42 | - |
4-pentene-1,2-diol | in 50 mM sodium phosphate buffer, at pH 7.5 and 30°C | Streptomyces coelicolor | |
52 | - |
1,2-pentanediol | pH 7.5, 30°C | Streptomyces coelicolor | |
52 | - |
1,2-pentanediol | in 50 mM sodium phosphate buffer, at pH 7.5 and 30°C | Streptomyces coelicolor | |
83 | - |
1-phenyl-1,2-ethanediol | pH 7.5, 30°C | Streptomyces coelicolor | |
83 | - |
1-phenyl-1,2-ethanediol | in 50 mM sodium phosphate buffer, at pH 7.5 and 30°C | Streptomyces coelicolor | |
86 | - |
(S)-1-phenyl-1,2-ethanediol | pH 7.5, 30°C | Streptomyces coelicolor | |
86 | - |
(S)-1-phenyl-1,2-ethanediol | in 50 mM sodium phosphate buffer, at pH 7.5 and 30°C | Streptomyces coelicolor | |
97 | - |
1,2-hexanediol | pH 7.5, 30°C | Streptomyces coelicolor | |
97 | - |
1,2-hexanediol | in 50 mM sodium phosphate buffer, at pH 7.5 and 30°C | Streptomyces coelicolor | |
101 | - |
(R)-1-phenyl-1,2-ethanediol | in 50 mM sodium phosphate buffer, at pH 7.5 and 30°C | Streptomyces coelicolor | |
150 | - |
1,2-Butanediol | pH 7.5, 30°C | Streptomyces coelicolor | |
150 | - |
1,2-Butanediol | in 50 mM sodium phosphate buffer, at pH 7.5 and 30°C | Streptomyces coelicolor | |
170 | - |
1,2,4-butanetriol | pH 7.5, 30°C | Streptomyces coelicolor | |
170 | - |
1,2,4-butanetriol | in 50 mM sodium phosphate buffer, at pH 7.5 and 30°C | Streptomyces coelicolor | |
250 | - |
3-butene-1,2-diol | pH 7.5, 30°C | Streptomyces coelicolor | |
250 | - |
3-butene-1,2-diol | in 50 mM sodium phosphate buffer, at pH 7.5 and 30°C | Streptomyces coelicolor | |
350 | - |
glycerol | pH 7.5, 30°C | Streptomyces coelicolor | |
350 | - |
glycerol | in 50 mM sodium phosphate buffer, at pH 7.5 and 30°C | Streptomyces coelicolor | |
430 | - |
L-arabinose | pH 7.5, 30°C | Streptomyces coelicolor | |
430 | - |
L-arabinose | in 50 mM sodium phosphate buffer, at pH 7.5 and 30°C | Streptomyces coelicolor | |
480 | - |
3-butenol | pH 7.5, 30°C | Streptomyces coelicolor | |
480 | - |
3-butenol | in 50 mM sodium phosphate buffer, at pH 7.5 and 30°C | Streptomyces coelicolor |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Streptomyces coelicolor | - |
- |
- |
Streptomyces coelicolor A3(2) | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Streptomyces coelicolor |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
an alditol + O2 = an aldose + H2O2 | proposed mechanism for the oxidation of 1,2-diols to the alpha-hydroxy acids, overview | Streptomyces coelicolor |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
17.3 | - |
substrate xylitol | Streptomyces coelicolor |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
(R)-1-phenyl-1,2-ethanediol + O2 | - |
Streptomyces coelicolor | ? | - |
? | |
(R)-1-phenyl-1,2-ethanediol + O2 | - |
Streptomyces coelicolor | hydroxy(phenyl)acetic acid + H2O2 | product identification by NMR | ? | |
(R)-1-phenyl-1,2-ethanediol + O2 | the enzyme is highly enantioselective for the oxidation of (R)-1-phenyl-1,2-ethanediol | Streptomyces coelicolor | mandelic acid + H2O2 | - |
? | |
(S)-1-phenyl-1,2-ethanediol + O2 | - |
Streptomyces coelicolor | ? | - |
? | |
1,2,4-butanetriol + O2 | - |
Streptomyces coelicolor | ? | - |
? | |
1,2-butanediol + O2 | - |
Streptomyces coelicolor | ? | - |
? | |
1,2-hexanediol + O2 | - |
Streptomyces coelicolor | ? | - |
? | |
1,2-pentanediol + O2 | - |
Streptomyces coelicolor | 2-hydroxypentanoic acid + H2O2 | product identification by NMR | ? | |
1,2-pentanediol + O2 | 50% conversion after 22 h | Streptomyces coelicolor | 2-hydroxypentanoic acid + H2O2 | - |
? | |
1,2-propanediol + O2 | - |
Streptomyces coelicolor | ? | - |
? | |
1,3,5-pentanetriol + O2 | - |
Streptomyces coelicolor | ? | - |
? | |
1,3-butanediol + O2 | - |
Streptomyces coelicolor | 3-hydroxybutanal + H2O2 | - |
? | |
1,3-butanediol + O2 | - |
Streptomyces coelicolor | 3-hydroxybutanal + H2O2 | product identification by GC-MS | ? | |
1,4-butanediol + O2 | very poor substrate | Streptomyces coelicolor | ? | - |
? | |
1-phenyl-1,2-ethanediol + O2 | the enzyme is highly enantioselective for the oxidation of 1-phenyl-1,2-ethanediol, 35% conversion to mandelic acid and two minor by-products (less than 5%) is observed after 65 h | Streptomyces coelicolor | ? | - |
? | |
2-amino-1-pentanol + O2 | - |
Streptomyces coelicolor | ? | - |
? | |
3-butene-1,2-diol + O2 | - |
Streptomyces coelicolor | ? | - |
? | |
3-butenol + O2 | - |
Streptomyces coelicolor | ? | - |
? | |
4-pentene-1,2-diol + O2 | - |
Streptomyces coelicolor | ? | - |
? | |
cis-2-butene-1,4-diol + O2 | - |
Streptomyces coelicolor | ? | - |
? | |
D-galactose + O2 | very poor substrate | Streptomyces coelicolor | ? | - |
? | |
D-mannitol + O2 | - |
Streptomyces coelicolor | ? | - |
? | |
D-sorbitol + O2 | - |
Streptomyces coelicolor | ? | - |
? | |
glycerol + O2 | - |
Streptomyces coelicolor | ? | - |
? | |
L-arabinose + O2 | - |
Streptomyces coelicolor | ? | - |
? | |
L-arabinose + O2 | very poor substrate | Streptomyces coelicolor | ? | - |
? | |
L-threitol + O2 | - |
Streptomyces coelicolor | ? | - |
? | |
additional information | substrate specificity, besides alditols, 1,2-diols are reasonable substrates indicating that two adjacent hydroxy groups at C-1 and C-2 seem to be a minimal requirement for a compound in order to be effectively oxidized by AldO, overview | Streptomyces coelicolor | ? | - |
? | |
additional information | the enzyme is able to perform oxidations of alcohols into aldehydes/ketones (single oxidation) and oxidations of alcohols into acids (double oxidation) | Streptomyces coelicolor | ? | - |
? | |
xylitol + O2 | best substrate | Streptomyces coelicolor | ? | - |
? | |
xylitol + O2 | best substrate | Streptomyces coelicolor | D-xylose + H2O2 | - |
? |
Synonyms | Comment | Organism |
---|---|---|
AldO | - |
Streptomyces coelicolor |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
55 | - |
- |
Streptomyces coelicolor |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
half-life of 40 h | Streptomyces coelicolor |
37 | 50 | the enzyme has a half-life of 40 h at 37°C and 4 h at 50°C | Streptomyces coelicolor |
50 | - |
half-life of 4 h | Streptomyces coelicolor |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.008 | - |
(S)-1-phenyl-1,2-ethanediol | pH 7.5, 30°C | Streptomyces coelicolor | |
0.008 | - |
(S)-1-phenyl-1,2-ethanediol | in 50 mM sodium phosphate buffer, at pH 7.5 and 30°C | Streptomyces coelicolor | |
0.017 | - |
2-amino-1-pentanol | pH 7.5, 30°C | Streptomyces coelicolor | |
0.017 | - |
2-amino-1-pentanol | in 50 mM sodium phosphate buffer, at pH 7.5 and 30°C | Streptomyces coelicolor | |
0.1 | - |
3-butenol | pH 7.5, 30°C | Streptomyces coelicolor | |
0.1 | - |
3-butenol | in 50 mM sodium phosphate buffer, at pH 7.5 and 30°C | Streptomyces coelicolor | |
0.29 | - |
1,2-Butanediol | pH 7.5, 30°C | Streptomyces coelicolor | |
0.29 | - |
1,2-Butanediol | in 50 mM sodium phosphate buffer, at pH 7.5 and 30°C | Streptomyces coelicolor | |
0.34 | - |
3-butene-1,2-diol | pH 7.5, 30°C | Streptomyces coelicolor | |
0.34 | - |
3-butene-1,2-diol | in 50 mM sodium phosphate buffer, at pH 7.5 and 30°C | Streptomyces coelicolor | |
0.35 | - |
4-pentene-1,2-diol | pH 7.5, 30°C | Streptomyces coelicolor | |
0.35 | - |
4-pentene-1,2-diol | in 50 mM sodium phosphate buffer, at pH 7.5 and 30°C | Streptomyces coelicolor | |
0.36 | - |
1-phenyl-1,2-ethanediol | pH 7.5, 30°C | Streptomyces coelicolor | |
0.36 | - |
1-phenyl-1,2-ethanediol | in 50 mM sodium phosphate buffer, at pH 7.5 and 30°C | Streptomyces coelicolor | |
0.74 | - |
(R)-1-phenyl-1,2-ethanediol | pH 7.5, 30°C | Streptomyces coelicolor | |
0.74 | - |
(R)-1-phenyl-1,2-ethanediol | in 50 mM sodium phosphate buffer, at pH 7.5 and 30°C | Streptomyces coelicolor | |
0.85 | - |
1,2-pentanediol | pH 7.5, 30°C | Streptomyces coelicolor | |
0.85 | - |
1,2-pentanediol | in 50 mM sodium phosphate buffer, at pH 7.5 and 30°C | Streptomyces coelicolor | |
1.6 | - |
glycerol | pH 7.5, 30°C | Streptomyces coelicolor | |
1.6 | - |
glycerol | in 50 mM sodium phosphate buffer, at pH 7.5 and 30°C | Streptomyces coelicolor | |
1.7 | - |
L-arabinose | pH 7.5, 30°C | Streptomyces coelicolor | |
1.7 | - |
L-arabinose | in 50 mM sodium phosphate buffer, at pH 7.5 and 30°C | Streptomyces coelicolor | |
2 | - |
1,2-hexanediol | pH 7.5, 30°C | Streptomyces coelicolor | |
2 | - |
1,2-hexanediol | in 50 mM sodium phosphate buffer, at pH 7.5 and 30°C | Streptomyces coelicolor | |
4.4 | - |
1,2,4-butanetriol | pH 7.5, 30°C | Streptomyces coelicolor | |
4.4 | - |
1,2,4-butanetriol | in 50 mM sodium phosphate buffer, at pH 7.5 and 30°C | Streptomyces coelicolor | |
6.3 | - |
L-Threitol | pH 7.5, 30°C | Streptomyces coelicolor | |
6.3 | - |
L-Threitol | in 50 mM sodium phosphate buffer, at pH 7.5 and 30°C | Streptomyces coelicolor | |
9.2 | - |
D-mannitol | pH 7.5, 30°C | Streptomyces coelicolor | |
9.2 | - |
D-mannitol | in 50 mM sodium phosphate buffer, at pH 7.5 and 30°C | Streptomyces coelicolor | |
13 | - |
xylitol | pH 7.5, 30°C | Streptomyces coelicolor | |
13 | - |
xylitol | in 50 mM sodium phosphate buffer, at pH 7.5 and 30°C | Streptomyces coelicolor | |
17 | - |
D-sorbitol | pH 7.5, 30°C | Streptomyces coelicolor | |
17 | - |
D-sorbitol | in 50 mM sodium phosphate buffer, at pH 7.5 and 30°C | Streptomyces coelicolor |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
assay at | Streptomyces coelicolor |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
6 | 9 | - |
Streptomyces coelicolor |
6 | 9 | over 80% of maximal activity within this range | Streptomyces coelicolor |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0001 | - |
(S)-1-phenyl-1,2-ethanediol | in 50 mM sodium phosphate buffer, at pH 7.5 and 30°C | Streptomyces coelicolor | |
0.0002 | - |
3-butenol | in 50 mM sodium phosphate buffer, at pH 7.5 and 30°C | Streptomyces coelicolor | |
0.0003 | - |
D-galactose | in 50 mM sodium phosphate buffer, at pH 7.5 and 30°C | Streptomyces coelicolor | |
0.0003 | - |
1,2-propanediol | in 50 mM sodium phosphate buffer, at pH 7.5 and 30°C | Streptomyces coelicolor | |
0.0006 | - |
2-amino-1-pentanol | in 50 mM sodium phosphate buffer, at pH 7.5 and 30°C | Streptomyces coelicolor | |
0.0014 | - |
3-butene-1,2-diol | in 50 mM sodium phosphate buffer, at pH 7.5 and 30°C | Streptomyces coelicolor | |
0.0019 | - |
1,2-Butanediol | in 50 mM sodium phosphate buffer, at pH 7.5 and 30°C | Streptomyces coelicolor | |
0.004 | - |
L-arabinose | in 50 mM sodium phosphate buffer, at pH 7.5 and 30°C | Streptomyces coelicolor | |
0.0043 | - |
1-phenyl-1,2-ethanediol | in 50 mM sodium phosphate buffer, at pH 7.5 and 30°C | Streptomyces coelicolor | |
0.0046 | - |
glycerol | in 50 mM sodium phosphate buffer, at pH 7.5 and 30°C | Streptomyces coelicolor | |
0.0073 | - |
(R)-1-phenyl-1,2-ethanediol | in 50 mM sodium phosphate buffer, at pH 7.5 and 30°C | Streptomyces coelicolor | |
0.0078 | - |
1,3,5-pentanetriol | in 50 mM sodium phosphate buffer, at pH 7.5 and 30°C | Streptomyces coelicolor | |
0.0083 | - |
4-pentene-1,2-diol | in 50 mM sodium phosphate buffer, at pH 7.5 and 30°C | Streptomyces coelicolor | |
0.016 | - |
1,2-pentanediol | in 50 mM sodium phosphate buffer, at pH 7.5 and 30°C | Streptomyces coelicolor | |
0.021 | - |
1,2-hexanediol | in 50 mM sodium phosphate buffer, at pH 7.5 and 30°C | Streptomyces coelicolor | |
0.026 | - |
1,2,4-butanetriol | in 50 mM sodium phosphate buffer, at pH 7.5 and 30°C | Streptomyces coelicolor | |
0.25 | - |
L-Threitol | in 50 mM sodium phosphate buffer, at pH 7.5 and 30°C | Streptomyces coelicolor | |
0.26 | - |
D-mannitol | in 50 mM sodium phosphate buffer, at pH 7.5 and 30°C | Streptomyces coelicolor | |
12 | - |
D-sorbitol | in 50 mM sodium phosphate buffer, at pH 7.5 and 30°C | Streptomyces coelicolor | |
41 | - |
xylitol | in 50 mM sodium phosphate buffer, at pH 7.5 and 30°C | Streptomyces coelicolor |