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Literature summary for 1.1.3.4 extracted from

  • Yuivar, Y.; Barahona, S.; Alcaino, J.; Cifuentes, V.; Baeza, M.
    Biochemical and thermodynamical characterization of glucose oxidase, invertase, and alkaline phosphatase secreted by antarctic yeasts (2017), Front. Mol. Biosci., 4, 86 .
    View publication on PubMedView publication on EuropePMC
Search for more literature for 1.1.3.4

Application

Application Comment Organism
diagnostics glucose biosensor Sporidiobolus salmonicolor
diagnostics glucose biosensor Goffeauzyma gastrica
diagnostics glucose biosensor Leucosporidium creatinivorum
diagnostics glucose biosensor Goffeauzyma gilvescens
diagnostics glucose biosensor Cystobasidium laryngis

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.6
-
 beta-D-glucose 64°C, pH not specified in the publication Goffeauzyma gastrica

Localization

Localization Comment Organism GeneOntology No. Textmining
extracellular
-
 Sporidiobolus salmonicolor
-
-
extracellular
-
 Goffeauzyma gastrica
-
-
extracellular
-
 Leucosporidium creatinivorum
-
-
extracellular
-
 Goffeauzyma gilvescens
-
-
extracellular
-
 Cystobasidium laryngis
-
-

Metals/Ions

Metals/Ions Comment Organism Structure
CaCl2 the enzyme shows highest activity at 64°C and with 2 M CaCl2 Goffeauzyma gastrica

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
91000
-
 SDS-PAGE Goffeauzyma gastrica

Organism

Organism UniProt Comment Textmining
Cystobasidium laryngis
-
-
-
Goffeauzyma gastrica
-
-
-
Goffeauzyma gilvescens
-
-
-
Leucosporidium creatinivorum
-
-
-
Sporidiobolus salmonicolor
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Goffeauzyma gastrica
-
 Leucosporidium creatinivorum
-
 Cystobasidium laryngis

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
beta-D-glucose + O2
-
 Sporidiobolus salmonicolor D-glucono-1,5-lactone + H2O2
-
 ?
beta-D-glucose + O2
-
 Goffeauzyma gastrica D-glucono-1,5-lactone + H2O2
-
 ?
beta-D-glucose + O2
-
 Leucosporidium creatinivorum D-glucono-1,5-lactone + H2O2
-
 ?
beta-D-glucose + O2
-
 Goffeauzyma gilvescens D-glucono-1,5-lactone + H2O2
-
 ?
beta-D-glucose + O2
-
 Cystobasidium laryngis D-glucono-1,5-lactone + H2O2
-
 ?

Subunits

Subunits Comment Organism
? x * 91000, SDS-PAGE Goffeauzyma gastrica

Synonyms

Synonyms Comment Organism
GOD
-
 Sporidiobolus salmonicolor
GOD
-
 Goffeauzyma gastrica
GOD
-
 Leucosporidium creatinivorum
GOD
-
 Goffeauzyma gilvescens
GOD
-
 Cystobasidium laryngis

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
64
-
 with 2 M CaCl2 Goffeauzyma gastrica

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
10 74 10°C: 13% of maximal activity, 30°C: 28% of maximal activity, 74°C: about 60% of maximal activity Goffeauzyma gastrica

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
4 54 1 h, the enzyme retains at least 80% activity Goffeauzyma gastrica
30
-
 after 6 h of incubation the percentage of activity is 79% Goffeauzyma gastrica
47
-
 after 6 h of incubation the percentage of activity is 68% Goffeauzyma gastrica
54
-
 after 6 h of incubation the percentage of activity is 11% Goffeauzyma gastrica

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2300
-
 beta-D-glucose 64°C, pH not specified in the publication Goffeauzyma gastrica