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Literature summary for 1.1.3.4 extracted from

  • Jithendar, T.; Sairam, K.; Verma, V.
    Research journal of pharmaceutical, biological and chemical sciences purification, characterization, thermostability and shelf life studies of glucose oxidase from Aspergillus niger PIL7 (2015), Res. J. Pharm. Biol. Chem. Sci., 6, 1666-1678 .
No PubMed abstract available
Search for more literature for 1.1.3.4

Application

Application Comment Organism
analysis GOx is the main component in glucose biosensors for determination of glucose in industrial solutions and in body fluids such as blood and urine Aspergillus niger

Inhibitors

Inhibitors Comment Organism Structure
Ag+
-
 Aspergillus niger
Co2+
-
 Aspergillus niger
Hg2+
-
 Aspergillus niger
Ni2+
-
 Aspergillus niger
Pb2+
-
 Aspergillus niger
Zn2+
-
 Aspergillus niger

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
 additional information enzyme thermodynamics and kinetics Aspergillus niger
89.13
-
 beta-D-glucose pH 6.0, 37°C Aspergillus niger

Metals/Ions

Metals/Ions Comment Organism Structure
CuCl2 activates Aspergillus niger
KCN activates Aspergillus niger
additional information no effect by NaF on enzyme activity Aspergillus niger

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
160000
-
 gel filtration Aspergillus niger

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
beta-D-glucose + O2 Aspergillus niger
-
 D-glucono-1,5-lactone + H2O2
-
 ?
beta-D-glucose + O2 Aspergillus niger PIL7
-
 D-glucono-1,5-lactone + H2O2
-
 ?

Organism

Organism UniProt Comment Textmining
Aspergillus niger
-
-
-
Aspergillus niger PIL7
-
-
-

Purification (Commentary)

Purification (Comment) Organism
native enzyme 9.5fold from strain PIL7 by ammonium sulfate fractionation, dialysis, anion exchange chromatography, and gel filtration Aspergillus niger

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
28.81
-
 purified native enzyme, pH 6.0, 37°C Aspergillus niger

Storage Stability

Storage Stability Organism
-20°C, purified enzyme, 6 months without addition of additives, complete stable, no loss of activity Aspergillus niger

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2-deoxy-D-glucose + O2 10% activity compared to beta-D-glucose Aspergillus niger 2-deoxy-D-glucono-1,5-lactone + H2O2
-
 ?
2-deoxy-D-glucose + O2 10% activity compared to beta-D-glucose Aspergillus niger PIL7 2-deoxy-D-glucono-1,5-lactone + H2O2
-
 ?
3,6-methyl-D-glucose + O2 10% activity compared to beta-D-glucose Aspergillus niger 3-O,6-O-dimethyl-D-glucono-1,5-lactone + H2O2
-
 ?
3,6-methyl-D-glucose + O2 10% activity compared to beta-D-glucose Aspergillus niger PIL7 3-O,6-O-dimethyl-D-glucono-1,5-lactone + H2O2
-
 ?
4-deoxy-D-glucose + O2 7% activity compared to beta-D-glucose Aspergillus niger 4-deoxy-D-glucono-1,5-lactone + H2O2
-
 ?
4-deoxy-D-glucose + O2 7% activity compared to beta-D-glucose Aspergillus niger PIL7 4-deoxy-D-glucono-1,5-lactone + H2O2
-
 ?
4-O-methyl-D-glucose + O2 8% activity compared to beta-D-glucose Aspergillus niger 4-O-methyl-D-glucono-1,5-lactone + H2O2
-
 ?
6-deoxy-D-glucose + O2 12% activity compared to beta-D-glucose Aspergillus niger 6-deoxy-D-glucono-1,5-lactone + H2O2
-
 ?
beta-D-glucose + O2
-
 Aspergillus niger D-glucono-1,5-lactone + H2O2
-
 ?
beta-D-glucose + O2
-
 Aspergillus niger PIL7 D-glucono-1,5-lactone + H2O2
-
 ?
D-mannose + O2 9% activity compared to beta-D-glucose Aspergillus niger ? + H2O2
-
 ?
L-sorbose + O2 15% activity compared to beta-D-glucose Aspergillus niger ? + H2O2
-
 ?
additional information no activity with 2-deoxy-6-fluoro-D-glucose, 4,6-dimethyl-D-glucose, beta-deoxy-D-glucose, 6-O-methyl-D-glucose, D-glucono-delta-lactone, L-gulono-gamma-lactone, D-gulono-gamma-lactone, D-glucuronolactone, altrose, galactose, xylose, idose, cellobiose, D-kabinose, L-arabinose, or D-fructose Aspergillus niger ?
-
 ?
additional information no activity with 2-deoxy-6-fluoro-D-glucose, 4,6-dimethyl-D-glucose, beta-deoxy-D-glucose, 6-O-methyl-D-glucose, D-glucono-delta-lactone, L-gulono-gamma-lactone, D-gulono-gamma-lactone, D-glucuronolactone, altrose, galactose, xylose, idose, cellobiose, D-kabinose, L-arabinose, or D-fructose Aspergillus niger PIL7 ?
-
 ?

Subunits

Subunits Comment Organism
monomer 1 * 160000, SDS-PAGE Aspergillus niger

Synonyms

Synonyms Comment Organism
GOX
-
 Aspergillus niger

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
-
-
 Aspergillus niger

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
20 70 activity range, profile overview. About 90% of maximal activity at 20°C, 90% at 55°C, rapid deactivation above Aspergillus niger

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
additional information
-
 thermal inactivation thermodynamic parameters of GOx, overview Aspergillus niger
30
-
 purified enzyme, completely stable for at least 180 min Aspergillus niger
55
-
 purified enzyme, retains 57 % activity after 180 min Aspergillus niger
65
-
 purified enzyme, loss of 50% activity after 27 min Aspergillus niger
70
-
 purified enzyme, inactivation Aspergillus niger

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6
-
-
 Aspergillus niger

pH Range

pH Minimum pH Maximum Comment Organism
2.5 8.5 activity range, profile overview. 85% of maximal activity at pH 5.0, 51% activity at pH 7.0 Aspergillus niger

Cofactor

Cofactor Comment Organism Structure
FAD
-
 Aspergillus niger