Literature summary for 1.1.3.4 extracted from

Tribst, A.A.; Cota, J.; Murakami, M.T.; Cristianini, M.
Effects of high pressure homogenization on the activity, stability, kinetics and three-dimensional conformation of a glucose oxidase produced by Aspergillus niger (2014), PLoS ONE, 9, e103410 .

General Stability

General Stability	Organism
the enzyme shows a pH-dependent response to the high pressure homogenization treatment, with reduction or maintenance of activity at pH 4.5-6.0 and a remarkable activity increase (30-300%) at pH 6.5 at all tested temperatures. i.e. 15°C, 50°C and 75°C. The enzyme's thermal tolerance is reduced due to high pressure homogenization treatment and the storage for 24 h at high temperatures of 50°C and 75°C also causes a reduction of activity	Aspergillus niger

Organism

the enzyme shows a pH-dependent response to the high pressure homogenization treatment, with reduction or maintenance of activity at pH 4.5-6.0 and a remarkable activity increase (30-300%) at pH 6.5 at all tested temperatures. i.e. 15°C, 50°C and 75°C. The enzyme's thermal tolerance is reduced due to high pressure homogenization treatment and the storage for 24 h at high temperatures of 50°C and 75°C also causes a reduction of activity

Aspergillus niger

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
beta-D-glucose + O2	Aspergillus niger	-	D-glucono-1,5-lactone + H2O2	-	?

Organism

Organism	UniProt	Comment	Textmining
Aspergillus niger	-	-	-

Storage Stability

Storage Stability	Organism
storage for 24 h at high temperatures of 50°C and 75°C causes a reduction of activity	Aspergillus niger

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
beta-D-glucose + O2	-	Aspergillus niger	D-glucono-1,5-lactone + H2O2	-	?

Temperature Optimum [°C]

Temperature Optimum [°C]	Temperature Optimum Maximum [°C]	Comment	Organism
50	-	-	Aspergillus niger

Temperature Range [°C]

Temperature Minimum [°C]	Temperature Maximum [°C]	Comment	Organism
15	60	activity within this range at pH 5.0	Aspergillus niger

Temperature Stability [°C]

Temperature Stability Minimum [°C]	Temperature Stability Maximum [°C]	Comment	Organism
15	70	the enzyme shows a pH-dependent response to the high pressure homogenization treatment, with reduction or maintenance of activity at pH 4.5-6.0 and a remarkable activity increase (30-300%) at pH 6.5 at all tested temperatures. i.e. 15°C, 50°C and 75°C. The enzyme's thermal tolerance is reduced due to high pressure homogenization treatment and the storage for 24 h at high temperatures of 50°C and 75°C also causes a reduction of activity	Aspergillus niger

Turnover Number [1/s]

Turnover Number Minimum [1/s]	Turnover Number Maximum [1/s]	Substrate	Comment	Organism
0.00521	-	beta-D-glucose	pH 5.0, 50°C, 150 MPa	Aspergillus niger
0.00528	-	beta-D-glucose	pH 5.0, 50°C, 75 MPa	Aspergillus niger
0.02103	-	beta-D-glucose	pH 5.0, 50°C	Aspergillus niger

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
5	-	-	Aspergillus niger

pH Range

pH Minimum	pH Maximum	Comment	Organism
4.5	6.5	over 60% activity within this range at 50°C	Aspergillus niger

pH Stability

pH Stability	pH Stability Maximum	Comment	Organism
4.5	6	the enzyme shows a pH-dependent response to the high pressure homogenization treatment, with reduction or maintenance of activity at pH 4.5-6.0 and a remarkable activity increase (30-300%) at pH 6.5 at all tested temperatures. i.e. 15°C, 50°C and 75°C	Aspergillus niger

Cofactor

Cofactor	Comment	Organism	Structure
FAD	-	Aspergillus niger