Literature summary for 1.1.3.4 extracted from

Jan, U.; Husain, Q.
Preparation of a highly stable, very active and high-yield multilayered assembly of glucose oxidase using carbohydrate-specific polyclonal antibodies (2004), Biotechnol. Appl. Biochem., 39, 233-239.

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General Stability

General Stability	Organism
4.0 M urea, immunoaffinity-layered preparation retains 87% of original activity after 2 h and 40% of activity after 24 h of incubation, whereas the soluble enzyme loses all of its activity within 1 h of preincubation	Aspergillus niger
most stable, highly active and high-yield glucose oxidase preparations are optained by assembling the enzyme on small amounts of immunoaffinity support using glycosyl-specific polyclonal antibodies	Aspergillus niger

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
additional information	-	additional information	KM-value of immunoaffinity-layered glucose oxidase preparations remains unaltered	Aspergillus niger

Organic Solvent Stability

Organic Solvent	Comment	Organism
Acetone	50%, 37°C, 6 h, immunoeffinity immobilized enzyme preparation retains 72% of the original activity, soluble enzyme retains 18% of the initial activity	Aspergillus niger
dimethylformamide	50%, 37°C, 6 h, immunoeffinity immobilized enzyme preparation retains 99% of the original activity, soluble enzyme retains 63% of the initial activity	Aspergillus niger
dioxane	50%, 37°C, 6 h, immunoeffinity immobilized enzyme preparation retains 99% of the original activity, soluble enzyme retains 63% of the initial activity	Aspergillus niger
tetrahydrofuran	50%, 37°C, 6 h, immunoeffinity immobilized enzyme preparation retains 87% of the original activity, soluble enzyme retains 1% of the initial activity	Aspergillus niger

Organism

Organism	UniProt	Comment	Textmining
Aspergillus niger	-	-	-

Temperature Stability [°C]

Temperature Stability Minimum [°C]	Temperature Stability Maximum [°C]	Comment	Organism
60	-	affinity-layered preparation retains 55% of the original activity after 2 h of preincubation, soluble enzyme loses almost 80% activity in 15 min	Aspergillus niger

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Release 2023.1 (January 2023)