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Literature summary for 1.1.3.4 extracted from

  • Pazur, J.H.
    Glucose oxidase from Aspergillus niger (1966), Methods Enzymol., 9, 82-87.
No PubMed abstract available

General Stability

General Stability Organism
3°C, little loss of activity, 3 years Aspergillus niger

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
150000 153000 sedimentation and diffusion data Aspergillus niger

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
beta-D-glucose + O2 + H2O Aspergillus niger
-
D-glucono-1,5-lactone + H2O2
-
?

Organism

Organism UniProt Comment Textmining
Aspergillus niger
-
-
-

Posttranslational Modification

Posttranslational Modification Comment Organism
glycoprotein
-
Aspergillus niger

Purification (Commentary)

Purification (Comment) Organism
using dialysis, ammonium sulfate fractionation and column chromatography on DEAE-cellulose Aspergillus niger

Source Tissue

Source Tissue Comment Organism Textmining
mycelium
-
Aspergillus niger
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
-
Aspergillus niger

Storage Stability

Storage Stability Organism
3°C, purified enzyme, several years Aspergillus niger

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2-deoxy-D-glucose + O2 + H2O 20% relative activity to D-glucose Aspergillus niger 2-deoxy-D-glucono-1,5-lactone + H2O2
-
?
3-deoxy-D-glucose + O2 + H2O 1% relative activity to D-glucose Aspergillus niger 3-deoxy-D-glucono-1,5-lactone + H2O2
-
?
4-deoxy-D-glucose + O2 + H2O 2% relative activity to D-glucose Aspergillus niger 4-deoxy-D-glucono-1,5-lactone + H2O2
-
?
4-O-methy-D-glucose + O2 + H2O 15% relative activity to D-glucose Aspergillus niger 4-O-methyl-D-glucono-1,5-lactone + H2O2
-
?
6-deoxy-D-glucose + O2 + H2O 10% relative activity to D-glucose Aspergillus niger 6-deoxy-D-glucono-1,5-lactone + H2O2
-
?
6-O-methyl-D-glucose + O2 + H2O 1% relative activity to D-glucose Aspergillus niger 6-O-methyl-D-glucono-1,5-lactone + H2O2
-
?
alpha-D-glucose + O2 + H2O very slow reaction Aspergillus niger D-glucono-1,5-lactone + H2O2
-
?
beta-D-glucose + O2 + H2O
-
Aspergillus niger D-glucono-1,5-lactone + H2O2
-
?
beta-D-glucose + O2 + H2O highly specific Aspergillus niger D-glucono-1,5-lactone + H2O2
-
?
beta-D-glucose + O2 + H2O glucose is the primary substrate for the enzyme Aspergillus niger D-glucono-1,5-lactone + H2O2
-
?
mannose + O2 + H2O 1% relative activity to D-glucose Aspergillus niger ? + H2O2
-
?

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
additional information
-
the enzyme is very stable at cold temperatures Aspergillus niger

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
5.5
-
-
Aspergillus niger

Cofactor

Cofactor Comment Organism Structure
FAD 2 mol per mol of enzyme Aspergillus niger