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Literature summary for 1.1.3.38 extracted from

  • van den Heuvel, R.H.; Fraaije, M.W.; Ferrer, M.; Mattevi, A.; van Berkel, W.J.
    Inversion of stereospecificity of vanillyl-alcohol oxidase (2000), Proc. Natl. Acad. Sci. USA, 97, 9455-9460.
    View publication on PubMedView publication on EuropePMC
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Crystallization (Commentary)

Crystallization (Comment) Organism
crystals of mutant enzyme D170S/T457E are grown by using the hanging-drop vapor diffusion method Penicillium simplicissimum

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
55
-
 4-(Methoxymethyl)phenol pH 7.5, 25°Cm wild-type enzyme Penicillium simplicissimum
65
-
 4-(Methoxymethyl)phenol pH 7.5, 25°C, mutant enzyme D170A/T457E Penicillium simplicissimum
168
-
 4-(Methoxymethyl)phenol pH 7.5, 25°C, mutant enzyme T457E Penicillium simplicissimum
238
-
 4-(Methoxymethyl)phenol pH 7.5, 25°C, mutant enzyme D170S/T457E Penicillium simplicissimum

Organism

Organism UniProt Comment Textmining
Penicillium simplicissimum P56216
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4-(methoxymethyl)phenol + O2 + H2O
-
 Penicillium simplicissimum 4-hydroxybenzaldehyde + methanol + H2O2 like native enzyme the mutant enzymes T457E, D170A and d170S preferentially form the (R)-enantiomer. The mutant enzymes D170A/T457E and D170S/T457E exhibit an inverted stereospecificity ?
4-ethylphenol + O2 + H2O
-
 Penicillium simplicissimum (R)-1-(4'-hydroxyphenyl)ethanol + H2O2 predominant product of wild-type enzyme and of mutant T457E ir
4-ethylphenol + O2 + H2O
-
 Penicillium simplicissimum (S)-1-(4'-hydroxyphenyl)ethanol + H2O2 predominant product of mutant D170S/T457E ir

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.02
-
 4-(Methoxymethyl)phenol pH 7.5, 25°C, mutant enzyme D170A/T457E Penicillium simplicissimum
0.05
-
 4-(Methoxymethyl)phenol pH 7.5, 25°C, mutant enzyme D170S/T457E Penicillium simplicissimum
1.3
-
 4-(Methoxymethyl)phenol pH 7.5, 25°C, mutant enzyme T457E Penicillium simplicissimum
3.1
-
 4-(Methoxymethyl)phenol pH 7.5, 25°Cm wild-type enzyme Penicillium simplicissimum