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Literature summary for 1.1.3.30 extracted from

  • Hirota-Mamoto, R.; Nagai, R.; Tachibana, S.; Yasuda, M.; Tani, A.; Kimbara, K.; Kawai, F.
    Cloning and expression of the gene for periplasmic poly(vinyl alcohol) dehydrogenase from Sphingomonas sp. strain 113P3, a novel-type quinohaemoprotein alcohol dehydrogenase (2006), Microbiology, 152, 1941-1949.
    View publication on PubMed

Activating Compound

Activating Compound Comment Organism Structure
pyrroloquinoline quinone absolutely required for activity, one mole of PQQ is bound to one mole of apoenzyme Sphingopyxis sp.

Cloned(Commentary)

Cloned (Comment) Organism
gene pvadh, DNA and amino acid sequence determination and analysis, sequence comparison, expression of His6-tagged enzyme in Escherichia coli Sphingopyxis sp.

Localization

Localization Comment Organism GeneOntology No. Textmining
periplasm
-
Sphingopyxis sp.
-
-

Metals/Ions

Metals/Ions Comment Organism Structure
Ca2+ high activation Sphingopyxis sp.
Mg2+ activates, the activation by Mg2+ is less than 80% of the activation by Ca2+ Sphingopyxis sp.

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
68000
-
recombinant enzyme, gel filtration Sphingopyxis sp.

Organism

Organism UniProt Comment Textmining
Sphingopyxis sp. Q588Z1 gene pvadh; gene pvadh or pvaA
-

Purification (Commentary)

Purification (Comment) Organism
recombinant His6-tagged enzyme from Escherichia coli 44fold to homogeneity Sphingopyxis sp.

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
18
-
purified recombinant enzyme, substrate PVA117 Sphingopyxis sp.

Storage Stability

Storage Stability Organism
4°C, purified recombinant enzyme, 20 mM Tris/HCl buffer, pH 8.0, stable for more than a month Sphingopyxis sp.

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1,3-butanediol + O2 low activity Sphingopyxis sp. ?
-
?
2,4-pentanediol + O2 low activity Sphingopyxis sp. ?
-
?
cyclohexanediol + O2 low activity Sphingopyxis sp. ?
-
?
additional information no activity with primary nor secondary alcohols Sphingopyxis sp. ?
-
?
polypropylene glycol + O2
-
Sphingopyxis sp. ?
-
?
polyvinyl alcohol + O2 substrate PVA117 Sphingopyxis sp. oxidized polyvinyl alcohol + H2O2
-
?
pyrroloquinoline quinone + O2 low activity Sphingopyxis sp. ?
-
?

Subunits

Subunits Comment Organism
monomer 1 * 68000, recombinant enzyme, SDS-PAGE Sphingopyxis sp.
More the enzyme possesses a conserved superbarrel domain SD, probable PQQ-binding amino acids in the SD and a hem-binding domain HBD Sphingopyxis sp.

Synonyms

Synonyms Comment Organism
periplasmic poly(vinyl alcohol) dehydrogenase
-
Sphingopyxis sp.
PVA dehydrogenase
-
Sphingopyxis sp.
PVADH
-
Sphingopyxis sp.

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
recombinant enzyme Sphingopyxis sp.

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.2 7.4 dependent on buffer system, recombinant enzyme Sphingopyxis sp.